##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062732_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7739808
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229083977276957	32.0	32.0	32.0	32.0	32.0
2	31.271357248138454	32.0	32.0	32.0	32.0	32.0
3	31.36448035920271	32.0	32.0	32.0	32.0	32.0
4	31.465088022855348	32.0	32.0	32.0	32.0	32.0
5	31.38447297400659	32.0	32.0	32.0	32.0	32.0
6	34.90670427483472	36.0	36.0	36.0	36.0	36.0
7	34.93453597298538	36.0	36.0	36.0	36.0	36.0
8	34.86443978972088	36.0	36.0	36.0	32.0	36.0
9	34.990357771148844	36.0	36.0	36.0	36.0	36.0
10	34.828696913411804	36.0	36.0	36.0	32.0	36.0
11	35.014347771934396	36.0	36.0	36.0	36.0	36.0
12	34.91730079092401	36.0	36.0	36.0	32.0	36.0
13	34.96920401643038	36.0	36.0	36.0	36.0	36.0
14	34.91551689654317	36.0	36.0	36.0	32.0	36.0
15	34.88368406554788	36.0	36.0	36.0	32.0	36.0
16	34.90087183041233	36.0	36.0	36.0	32.0	36.0
17	34.87352942088486	36.0	36.0	36.0	32.0	36.0
18	34.87440954607659	36.0	36.0	36.0	32.0	36.0
19	34.87028812601036	36.0	36.0	36.0	32.0	36.0
20	34.85728160181751	36.0	36.0	36.0	32.0	36.0
21	34.853229175710815	36.0	36.0	36.0	32.0	36.0
22	34.83614826104213	36.0	36.0	36.0	32.0	36.0
23	34.783194105073406	36.0	36.0	36.0	32.0	36.0
24	34.76062248572575	36.0	36.0	36.0	32.0	36.0
25	34.74907775490038	36.0	36.0	36.0	32.0	36.0
26	34.68342948558931	36.0	36.0	36.0	32.0	36.0
27	34.66438986600184	36.0	36.0	36.0	32.0	36.0
28	34.645337713803755	36.0	36.0	36.0	32.0	36.0
29	34.61252126667742	36.0	36.0	36.0	32.0	36.0
30	34.593558780786296	36.0	36.0	36.0	32.0	36.0
31	34.58885233328785	36.0	36.0	36.0	32.0	36.0
32	34.56765671706585	36.0	36.0	36.0	32.0	36.0
33	34.55073743431362	36.0	36.0	36.0	32.0	36.0
34	34.54160968850907	36.0	36.0	36.0	32.0	36.0
35	34.522221223058764	36.0	36.0	36.0	32.0	36.0
36	34.495215126783506	36.0	36.0	36.0	32.0	36.0
37	34.49100985450802	36.0	36.0	36.0	32.0	36.0
38	34.07424899945838	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	10.0
20	43.0
21	180.0
22	565.0
23	1800.0
24	5016.0
25	12280.0
26	25779.0
27	49617.0
28	86369.0
29	136737.0
30	207911.0
31	303295.0
32	438651.0
33	710738.0
34	1759282.0
35	4001531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36069367985971	18.467251883027707	12.26071605963046	26.911338377482124
2	15.66131873038711	20.269184972030313	37.40256864252963	26.66692765505294
3	18.07546130343285	24.132304057154908	28.637984301419362	29.15425033799288
4	12.261317998562236	16.435872470023757	36.10009876229296	35.20271076912105
5	14.205164083591255	36.88064492580885	33.49189029956181	15.422300691038082
6	34.182317372541924	35.715251040078556	16.81040853768831	13.292023049691206
7	30.4054097703628	30.483202739511515	20.68247729197261	18.428910198153073
8	27.656490584355637	33.77101637690434	19.108537513280694	19.46395552545933
9	27.069891733302114	14.922529223144807	18.38806362013872	39.619515423414356
10	15.364469607506196	27.002935499125293	31.920232462589183	25.71236243077933
11	37.05928830262353	21.316538717223654	22.66428146422487	18.959891515927946
12	24.89192526457137	23.472570225806074	28.7502246510012	22.88527985862136
13	28.973061682657402	19.812312809916236	25.38058834390015	25.834037163526208
14	23.220088046123113	19.904113888111162	25.199659371351252	31.676138694414472
15	24.90387358446101	27.49612135081387	22.10510906730503	25.49489599742009
16	25.681809813037926	26.11450805285146	23.508654287801825	24.69502784630879
17	24.04305119713564	26.169938582455792	25.058722386911924	24.728287833496644
18	24.72136259710835	25.313883755255944	25.902412566306555	24.06234108132915
19	25.418459992806024	25.22705989605944	25.287629874022716	24.066850237111826
20	25.509198677796658	24.9371043829511	24.89718091198128	24.65651602727096
21	26.15098302965827	24.667442828280898	24.6402069718366	24.54136717022423
22	25.416987303554457	24.818199530634107	24.941174220949264	24.823638944862168
23	24.273329861232686	24.658689104137306	25.457031074870113	25.610949959759893
24	24.730613472582267	25.236194489579074	25.095868011196142	24.93732402664252
25	24.824016838671476	24.836872463032975	25.30913254510483	25.02997815319072
26	24.57202415667783	25.420921402939555	25.53729382881173	24.469760611570884
27	25.153618449485666	25.227716038400512	24.668773441356894	24.94989207075693
28	24.491111407414756	25.074924339208415	25.41878299823458	25.015181255142245
29	24.698054525383576	25.178415278518536	25.209113714448733	24.914416481649155
30	24.67324770847029	25.191593900003724	25.458939033112966	24.676219358413025
31	25.046060574112435	24.95435287283612	24.81875519392729	25.180831359124156
32	24.722874262514004	25.157109840450826	24.77341815197483	25.346597745060347
33	24.412646928709343	24.923421872997366	25.250212925178506	25.41371827311478
34	24.962595971372934	24.944895273887933	25.484921589786207	24.60758716495293
35	25.418925120623147	24.85172758807454	25.26300394014942	24.466343351152897
36	24.66076677871079	25.165675944416193	25.108684866601344	25.064872410271676
37	25.41118591055489	24.962440928767222	24.79344448854545	24.832928672132436
38	24.680240569430513	25.016295634397927	25.23074553706341	25.072718259108147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	112.5
2	182.0
3	182.0
4	552.5
5	923.0
6	923.0
7	1104.5
8	1286.0
9	1366.5
10	1447.0
11	1447.0
12	2249.5
13	3052.0
14	5241.5
15	7431.0
16	7431.0
17	12538.0
18	17645.0
19	17645.0
20	24538.5
21	31432.0
22	41836.5
23	52241.0
24	52241.0
25	67962.0
26	83683.0
27	83683.0
28	109029.5
29	134376.0
30	164261.5
31	194147.0
32	194147.0
33	242287.5
34	290428.0
35	290428.0
36	325585.5
37	360743.0
38	404411.5
39	448080.0
40	448080.0
41	480864.5
42	513649.0
43	565384.5
44	617120.0
45	617120.0
46	632976.5
47	648833.0
48	648833.0
49	686447.0
50	724061.0
51	730043.5
52	736026.0
53	736026.0
54	710275.0
55	684524.0
56	684524.0
57	651988.5
58	619453.0
59	555966.5
60	492480.0
61	492480.0
62	450609.0
63	408738.0
64	338429.0
65	268120.0
66	268120.0
67	220617.0
68	173114.0
69	173114.0
70	136341.0
71	99568.0
72	76389.5
73	53211.0
74	53211.0
75	39403.5
76	25596.0
77	25596.0
78	23529.5
79	21463.0
80	16649.0
81	11835.0
82	11835.0
83	10857.5
84	9880.0
85	9880.0
86	6316.0
87	2752.0
88	2157.5
89	1563.0
90	1563.0
91	964.0
92	365.0
93	273.5
94	182.0
95	182.0
96	126.5
97	71.0
98	71.0
99	68.0
100	65.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00873406678822007
2	0.0
3	0.0
4	1.5504260570804856E-4
5	3.876065142701214E-5
6	1.0336173713869904E-4
7	4.392873828394709E-4
8	0.002441921039901765
9	0.003992347096982251
10	8.785747656789418E-4
11	0.005465251851208712
12	3.230054285584345E-4
13	1.9380325713506074E-4
14	1.292021714233738E-5
15	0.0
16	3.876065142701214E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.421223885657112E-4
22	5.168086856934952E-5
23	2.1964369141973546E-4
24	0.0
25	6.460108571168691E-5
26	2.584043428467476E-5
27	1.9380325713506074E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.460108571168691E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	7739808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.35380805806932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58196857853771	38.48091233529916
2	11.100921488020104	10.735436537988427
3	3.495298671865646	5.0703300325504825
4	1.6069072573537049	3.108003403567986
5	0.946498623811853	2.288340639151255
6	0.5817191161855332	1.6877000692646995
7	0.42911948634017977	1.4524692893529245
8	0.2979571168596437	1.1525888990533553
9	0.24515667585269865	1.066883296350214
>10	1.5026054690309403	13.637877024688267
>50	0.1233369429402424	4.100660473897637
>100	0.07174354215202723	6.795949732453632
>500	0.00920458658326016	3.1784759940387977
>1k	0.0073202309442361516	6.566458801744853
>5k	2.4221352227937211E-4	0.6779134705983983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.168086856934953E-5
2	0.0	1.2920217142337382E-5	0.0	0.0	5.168086856934953E-5
3	0.0	1.2920217142337382E-5	0.0	0.0	5.168086856934953E-5
4	0.0	1.2920217142337382E-5	0.0	0.0	5.168086856934953E-5
5	0.0	3.876065142701214E-5	0.0	0.0	6.460108571168691E-5
6	0.0	3.876065142701214E-5	0.0	0.0	1.421223885657112E-4
7	0.0	3.876065142701214E-5	0.0	0.0	1.5504260570804856E-4
8	0.0	3.876065142701214E-5	0.0	1.2920217142337382E-5	1.5504260570804856E-4
9	0.0	3.876065142701214E-5	0.0	2.5840434284674764E-5	1.6796282285038596E-4
10	0.0	3.876065142701214E-5	0.0	6.460108571168691E-5	1.6796282285038596E-4
11	0.0	3.876065142701214E-5	0.0	6.460108571168691E-5	1.6796282285038596E-4
12	0.0	3.876065142701214E-5	0.0	6.460108571168691E-5	3.876065142701214E-4
13	0.0	3.876065142701214E-5	0.0	6.460108571168691E-5	5.297289028358326E-4
14	0.0	3.876065142701214E-5	0.0	7.752130285402428E-5	5.297289028358326E-4
15	0.0	3.876065142701214E-5	0.0	1.1628195428103643E-4	6.589310742592064E-4
16	1.2920217142337382E-5	3.876065142701214E-5	0.0	1.5504260570804856E-4	7.364523771132307E-4
17	1.2920217142337382E-5	3.876065142701214E-5	0.0	1.6796282285038596E-4	7.364523771132307E-4
18	1.2920217142337382E-5	3.876065142701214E-5	0.0	2.842447771314224E-4	7.752130285402428E-4
19	1.2920217142337382E-5	3.876065142701214E-5	0.0	3.4884586284310927E-4	7.752130285402428E-4
20	1.2920217142337382E-5	3.876065142701214E-5	0.0	4.134469485547962E-4	8.785747656789419E-4
21	1.2920217142337382E-5	3.876065142701214E-5	0.0	5.297289028358326E-4	9.302556342482914E-4
22	1.2920217142337382E-5	3.876065142701214E-5	0.0	6.201704228321942E-4	9.690162856753036E-4
23	1.2920217142337382E-5	3.876065142701214E-5	0.0	7.235321599708933E-4	9.690162856753036E-4
24	1.2920217142337382E-5	3.876065142701214E-5	0.0	9.690162856753036E-4	9.81936502817641E-4
25	1.2920217142337382E-5	3.876065142701214E-5	0.0	0.0012015801942373765	9.948567199599783E-4
26	1.2920217142337382E-5	3.876065142701214E-5	0.0	0.0014858249713687987	0.0010982184570986775
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6750	0.0	21.761065	1
GGTATCA	2290	0.0	19.354656	1
CGTAACG	80	5.629853E-5	15.999231	14
TCTAGCG	1345	0.0	14.393361	28
ACCGTCG	1215	0.0	14.224296	8
TCAACGC	10265	0.0	14.16941	4
TATCAAC	10430	0.0	14.066892	2
CTAGCGG	1370	0.0	14.013925	29
TACCGTC	1295	0.0	13.839945	7
CAACGCA	10525	0.0	13.82054	5
CCGATAA	665	0.0	13.71593	9
CGTCGTA	1275	0.0	13.553778	10
CGCGTAA	665	0.0	13.474516	10
AACGCAG	10805	0.0	13.462483	6
ATCAACG	10870	0.0	13.424671	3
CCGTCGT	1305	0.0	13.242968	9
GTATTAG	1760	0.0	13.182462	1
ATACCGT	1400	0.0	13.144858	6
ACGAACG	670	0.0	13.1336975	15
TAGCGGC	1465	0.0	12.995963	30
>>END_MODULE