FastQCFastQC Report
Thu 2 Feb 2017
SRR4062731_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062731_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772917
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22800.020.6543851
GGTATCA9150.019.2562351
TATACCG701.7922945E-518.2815025
ATCGTTT4100.014.82825729
CGTCGTA4750.014.82111110
ACCGTCG4550.014.7676928
TACCGTC4700.014.6367717
AACCGCG3950.014.5807587
GTCCTAA7250.014.139751
CGAAAAC5700.013.75394323
ATACCGT5350.013.7538396
CCGTTAA2450.013.71458816
GTAATAC1751.1750672E-913.712363
CGTTTAT4450.013.66223631
CCGTCGT5050.013.62381659
TCAACGC33700.013.4333484
TCTAGCG5500.013.38066528
CATCGTT4800.013.33218428
GGGTATA1453.1142008E-713.2560161
CGCGGTC4250.013.17652210
Produced by FastQC (version 0.11.5)