FastQCFastQC Report
Thu 2 Feb 2017
SRR4062731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772917
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.022.132321
GGTATCA8100.019.7532311
TTAACGC450.00885112717.778553
GTATCGA703.6960072E-416.001569
GGACCGT1304.803951E-916.001566
TTAGACG1153.347377E-715.3052884
CTAAGAC3400.015.0594783
CGCGCAT650.004164345614.76880629
TATCAAC35300.014.3225342
TCAACGC35350.014.1225084
ATAGAAC6600.014.0612173
GAACCGC4600.013.91446
AACCGCG3600.013.7791217
CTAGCGG5350.013.75661429
GTATTAA4100.013.6586361
ATCAACG36600.013.6399363
TCTAGCG5400.013.62923828
CAACGCA36700.013.5599065
CGCGTAA3850.013.29903910
TGTAGGA11700.013.2645762
Produced by FastQC (version 0.11.5)