##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2772917
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21797947792884	32.0	32.0	32.0	32.0	32.0
2	31.23840994880121	32.0	32.0	32.0	32.0	32.0
3	31.337392716767216	32.0	32.0	32.0	32.0	32.0
4	31.4399367164614	32.0	32.0	32.0	32.0	32.0
5	31.358337447532687	32.0	32.0	32.0	32.0	32.0
6	34.88981314622832	36.0	36.0	36.0	36.0	36.0
7	34.91282753865334	36.0	36.0	36.0	36.0	36.0
8	34.8453696955228	36.0	36.0	36.0	32.0	36.0
9	34.97020105542286	36.0	36.0	36.0	36.0	36.0
10	34.80213399824084	36.0	36.0	36.0	32.0	36.0
11	34.98508718436217	36.0	36.0	36.0	36.0	36.0
12	34.889851012489736	36.0	36.0	36.0	32.0	36.0
13	34.94322873710248	36.0	36.0	36.0	36.0	36.0
14	34.895497773644145	36.0	36.0	36.0	32.0	36.0
15	34.85515109179251	36.0	36.0	36.0	32.0	36.0
16	34.8789801497845	36.0	36.0	36.0	32.0	36.0
17	34.8493402435053	36.0	36.0	36.0	32.0	36.0
18	34.84937233966974	36.0	36.0	36.0	32.0	36.0
19	34.83924509821246	36.0	36.0	36.0	32.0	36.0
20	34.8299191789729	36.0	36.0	36.0	32.0	36.0
21	34.82566120803472	36.0	36.0	36.0	32.0	36.0
22	34.80179031683963	36.0	36.0	36.0	32.0	36.0
23	34.748730308191696	36.0	36.0	36.0	32.0	36.0
24	34.725548222323276	36.0	36.0	36.0	32.0	36.0
25	34.71695907234151	36.0	36.0	36.0	32.0	36.0
26	34.64877383636077	36.0	36.0	36.0	32.0	36.0
27	34.626169120821146	36.0	36.0	36.0	32.0	36.0
28	34.595382768398764	36.0	36.0	36.0	32.0	36.0
29	34.56717528869418	36.0	36.0	36.0	32.0	36.0
30	34.54119939399556	36.0	36.0	36.0	32.0	36.0
31	34.547287206937675	36.0	36.0	36.0	32.0	36.0
32	34.51843636142012	36.0	36.0	36.0	32.0	36.0
33	34.492107048281646	36.0	36.0	36.0	32.0	36.0
34	34.49145358479897	36.0	36.0	36.0	32.0	36.0
35	34.46843089785955	36.0	36.0	36.0	32.0	36.0
36	34.439377377685666	36.0	36.0	36.0	32.0	36.0
37	34.43241611631362	36.0	36.0	36.0	32.0	36.0
38	34.00732117117101	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	16.0
21	38.0
22	205.0
23	694.0
24	1821.0
25	4579.0
26	9460.0
27	18331.0
28	31910.0
29	50757.0
30	77260.0
31	112726.0
32	162377.0
33	261774.0
34	641353.0
35	1399614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31326870739222	18.514726217362735	12.106904742497676	27.065100332747377
2	15.79996804808799	20.77902079290509	36.99227203699209	26.428739122014832
3	18.017849073737153	24.604522962641866	28.800537484533432	28.57709047908755
4	12.579235323456109	16.814351416255334	36.00624037338378	34.60017288690477
5	14.400554795424597	36.70387902401589	33.25757668647495	15.637989494084561
6	33.77898933829947	35.553186609331114	17.123959038066786	13.543865014302634
7	30.032583157374663	30.390114338572726	21.173534614420618	18.403767889631993
8	27.536780682725926	33.445672420663904	19.53957202955077	19.4779748670594
9	27.31415530581436	14.762916966300807	18.471407870526317	39.45151985735852
10	15.732618797321932	26.6945917533841	31.81808182423063	25.75470762506334
11	36.752518054108954	21.04638095540354	23.197798884583538	19.003302105903973
12	25.240668093328804	23.22440789554352	28.81972919384934	22.715194817278338
13	29.25297638474513	19.792737668104024	25.2128899917812	25.741395955369644
14	23.563209271395166	19.960467608827674	24.99862960147368	31.477693518303475
15	25.02512696918083	27.553727717057523	21.963225008177307	25.457920305584338
16	25.68862525947414	25.730061783335668	23.712546647644572	24.86876630954562
17	24.25236673149611	26.023173430722952	24.99847633376693	24.725983504014003
18	24.92000301487567	24.966235916906278	26.05043713894069	24.063323929277363
19	25.805460459148254	24.850365156980896	25.065986468401324	24.27818791546952
20	25.806650541649823	24.70293917921092	24.618479384705708	24.871930894433554
21	26.399304125551676	24.431013727796824	24.3215620823437	24.8481200643078
22	25.837818383247097	24.71099016342363	24.60752507468672	24.843666378642556
23	24.403731528443746	24.578529718938068	25.191459375558978	25.82627937705921
24	25.17013671884157	24.952135242418002	24.882605573841555	24.995122464898877
25	25.032186357023694	24.592360239084226	25.18098289380774	25.194470510084336
26	24.78029454181283	25.277352333300996	25.35896314242366	24.58338998246251
27	25.308430048678034	25.02302631816205	24.475587775170812	25.1929558579891
28	24.658545495591827	24.926097679807942	25.13944701554356	25.275909809056675
29	24.968760334333844	24.897716015300855	24.833740065065054	25.299783585300244
30	24.697782155037455	25.03161832828029	25.26563182381586	25.00496769286639
31	25.33382715746631	24.653244218993933	24.593487652172787	25.419440971366974
32	24.98167092632055	24.98131029525947	24.5158077216159	25.521211056804084
33	24.649205151109825	24.716174339152598	25.12686099151183	25.507759518225754
34	25.2551374599384	24.682852029108695	25.27172648874813	24.790284022204776
35	25.64909083106346	24.682491398047617	25.081709982664464	24.58670778822446
36	24.841277254241653	25.002154770589964	24.911672437364697	25.244895537803693
37	25.54836657570349	24.8152757547377	24.51277842070282	25.123579248855986
38	24.899401856674963	24.989812161502304	24.889015670879083	25.221770310943647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	59.5
2	82.0
3	82.0
4	234.0
5	386.0
6	386.0
7	481.5
8	577.0
9	582.5
10	588.0
11	588.0
12	870.5
13	1153.0
14	1813.0
15	2473.0
16	2473.0
17	4122.5
18	5772.0
19	5772.0
20	7949.5
21	10127.0
22	13519.0
23	16911.0
24	16911.0
25	22285.5
26	27660.0
27	27660.0
28	36483.0
29	45306.0
30	57521.0
31	69736.0
32	69736.0
33	88568.0
34	107400.0
35	107400.0
36	118942.0
37	130484.0
38	145951.5
39	161419.0
40	161419.0
41	170983.0
42	180547.0
43	197640.5
44	214734.0
45	214734.0
46	220276.5
47	225819.0
48	225819.0
49	239987.0
50	254155.0
51	257414.5
52	260674.0
53	260674.0
54	252748.5
55	244823.0
56	244823.0
57	235285.5
58	225748.0
59	203206.5
60	180665.0
61	180665.0
62	166357.0
63	152049.0
64	125787.5
65	99526.0
66	99526.0
67	82400.5
68	65275.0
69	65275.0
70	51607.0
71	37939.0
72	29188.0
73	20437.0
74	20437.0
75	15298.5
76	10160.0
77	10160.0
78	9534.0
79	8908.0
80	6858.0
81	4808.0
82	4808.0
83	4546.0
84	4284.0
85	4284.0
86	2760.0
87	1236.0
88	989.0
89	742.0
90	742.0
91	457.0
92	172.0
93	107.0
94	42.0
95	42.0
96	41.0
97	40.0
98	40.0
99	31.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008763334784272303
2	0.0
3	0.0
4	2.1637863664869883E-4
5	1.0818931832434941E-4
6	3.606310610811647E-5
7	4.3275727329739766E-4
8	0.0022359125787032212
9	0.0032817426558385985
10	7.933883343785624E-4
11	0.005084897961244422
12	3.966941671892812E-4
13	2.1637863664869883E-4
14	3.606310610811647E-5
15	0.0
16	3.606310610811647E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0818931832434941E-4
22	3.606310610811647E-5
23	1.8031553054058234E-4
24	0.0
25	1.0818931832434941E-4
26	0.0
27	1.0818931832434941E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0818931832434941E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2772917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.00472454702512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7199137358984	44.94981345049746
2	10.401831770514097	11.442997826432448
3	2.9612239479209594	4.8864392273233985
4	1.4004479751210124	3.0812502045588146
5	0.787462515800175	2.165707938634802
6	0.5305269802593953	1.750889424835984
7	0.36272255737007525	1.3966018048593452
8	0.27786865554144924	1.2227271086647697
9	0.20647810105541387	1.022154396619127
>10	1.198720197114081	11.843166732776204
>50	0.07920853716060636	3.013458410072283
>100	0.06141267909698384	7.1730417310666486
>500	0.008692269315984838	3.269515755388719
>1k	0.003490077831390946	2.7822359882700494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.212621221623294E-5
2	0.0	0.0	0.0	0.0	7.212621221623294E-5
3	0.0	0.0	0.0	0.0	7.212621221623294E-5
4	0.0	0.0	0.0	0.0	1.0818931832434941E-4
5	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
6	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
7	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
8	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
9	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
10	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
11	0.0	3.606310610811647E-5	0.0	0.0	1.4425242443246589E-4
12	0.0	3.606310610811647E-5	0.0	7.212621221623294E-5	2.524417427568153E-4
13	0.0	3.606310610811647E-5	0.0	7.212621221623294E-5	3.2456795497304823E-4
14	0.0	3.606310610811647E-5	0.0	1.0818931832434941E-4	3.2456795497304823E-4
15	0.0	3.606310610811647E-5	0.0	1.4425242443246589E-4	6.1307280383798E-4
16	0.0	3.606310610811647E-5	0.0	1.8031553054058234E-4	6.491359099460965E-4
17	0.0	3.606310610811647E-5	0.0	2.1637863664869883E-4	6.491359099460965E-4
18	0.0	3.606310610811647E-5	0.0	2.8850484886493177E-4	7.212621221623294E-4
19	0.0	3.606310610811647E-5	0.0	2.8850484886493177E-4	7.212621221623294E-4
20	0.0	3.606310610811647E-5	0.0	3.966941671892812E-4	7.933883343785624E-4
21	0.0	3.606310610811647E-5	0.0	4.3275727329739766E-4	7.933883343785624E-4
22	0.0	3.606310610811647E-5	0.0	5.409465916217471E-4	7.933883343785624E-4
23	0.0	3.606310610811647E-5	0.0	7.933883343785624E-4	8.655145465947953E-4
24	0.0	3.606310610811647E-5	0.0	8.655145465947953E-4	9.015776527029118E-4
25	0.0	3.606310610811647E-5	0.0	0.0011900825015678435	9.015776527029118E-4
26	0.0	3.606310610811647E-5	0.0	0.0016228397748652413	9.376407588110282E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2270	0.0	22.13232	1
GGTATCA	810	0.0	19.753231	1
TTAACGC	45	0.008851127	17.77855	3
GTATCGA	70	3.6960072E-4	16.00156	9
GGACCGT	130	4.803951E-9	16.00156	6
TTAGACG	115	3.347377E-7	15.305288	4
CTAAGAC	340	0.0	15.059478	3
CGCGCAT	65	0.0041643456	14.768806	29
TATCAAC	3530	0.0	14.322534	2
TCAACGC	3535	0.0	14.122508	4
ATAGAAC	660	0.0	14.061217	3
GAACCGC	460	0.0	13.9144	6
AACCGCG	360	0.0	13.779121	7
CTAGCGG	535	0.0	13.756614	29
GTATTAA	410	0.0	13.658636	1
ATCAACG	3660	0.0	13.639936	3
TCTAGCG	540	0.0	13.629238	28
CAACGCA	3670	0.0	13.559906	5
CGCGTAA	385	0.0	13.299039	10
TGTAGGA	1170	0.0	13.264576	2
>>END_MODULE