FastQCFastQC Report
Thu 2 Feb 2017
SRR4062730_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062730_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320768
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT106820.1146042901185825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90650.022.276541
GGTATCA28900.021.1673281
TCGCGTA3000.017.0670539
CGCGTAA3150.016.76228510
GTCCTAC50250.016.0936971
TAGGACG45550.015.7332794
GTAGGAC49500.015.70717053
TATCAAC127400.015.563382
ATCAACG129400.015.1573433
TCAACGC129300.015.1553934
TAGGACC28050.015.055694
CAACGCA133100.014.7344085
AGGACGT47050.014.4493925
TCCTACA59750.014.4094182
AACGCAG137800.014.2667586
TTAGGAC30200.014.2499133
GCGTAAC3600.014.22269711
GGACGTG47500.014.0431686
TGTAGGA58000.013.9888512
GTCCTAA27750.013.9076761
Produced by FastQC (version 0.11.5)