##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062730_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9320768
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310260055823726	32.0	32.0	32.0	32.0	32.0
2	30.946106801499617	32.0	32.0	32.0	32.0	32.0
3	30.982636516647556	32.0	32.0	32.0	32.0	32.0
4	31.053929247032006	32.0	32.0	32.0	32.0	32.0
5	30.958478636095222	32.0	32.0	32.0	32.0	32.0
6	34.63149828426155	36.0	36.0	36.0	32.0	36.0
7	34.588677671196194	36.0	36.0	36.0	32.0	36.0
8	34.570922160062345	36.0	36.0	36.0	32.0	36.0
9	34.720652632916085	36.0	36.0	36.0	32.0	36.0
10	34.450106471913045	36.0	36.0	36.0	32.0	36.0
11	34.6932953378949	36.0	36.0	36.0	32.0	36.0
12	34.538218202620214	36.0	36.0	36.0	32.0	36.0
13	34.59871461235812	36.0	36.0	36.0	32.0	36.0
14	34.51871144094564	36.0	36.0	36.0	32.0	36.0
15	34.46485096507069	36.0	36.0	36.0	32.0	36.0
16	34.485679184376224	36.0	36.0	36.0	32.0	36.0
17	34.44008744772963	36.0	36.0	36.0	32.0	36.0
18	34.454324364687544	36.0	36.0	36.0	32.0	36.0
19	34.43920672631268	36.0	36.0	36.0	32.0	36.0
20	34.423959592170945	36.0	36.0	36.0	32.0	36.0
21	34.42107109628734	36.0	36.0	36.0	32.0	36.0
22	34.39152267280979	36.0	36.0	36.0	32.0	36.0
23	34.33974603809472	36.0	36.0	36.0	32.0	36.0
24	34.32868117734504	36.0	36.0	36.0	32.0	36.0
25	34.30445999728778	36.0	36.0	36.0	32.0	36.0
26	34.26932415869594	36.0	36.0	36.0	32.0	36.0
27	34.265671026250196	36.0	36.0	36.0	32.0	36.0
28	34.24553985251001	36.0	36.0	36.0	32.0	36.0
29	34.218974981460754	36.0	36.0	36.0	32.0	36.0
30	34.19173452230546	36.0	36.0	36.0	32.0	36.0
31	34.200042743258926	36.0	36.0	36.0	32.0	36.0
32	34.16947326658061	36.0	36.0	36.0	32.0	36.0
33	34.15127744838193	36.0	36.0	36.0	32.0	36.0
34	34.15075817786689	36.0	36.0	36.0	32.0	36.0
35	34.13010462228005	36.0	36.0	36.0	32.0	36.0
36	34.09066967443026	36.0	36.0	36.0	32.0	36.0
37	34.074181333555345	36.0	36.0	36.0	32.0	36.0
38	33.533998056812486	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	6.0
7	24.0
8	8.0
9	38.0
10	71.0
11	89.0
12	40.0
13	53.0
14	501.0
15	1478.0
16	2154.0
17	2642.0
18	3228.0
19	4107.0
20	5680.0
21	8351.0
22	13191.0
23	21374.0
24	33751.0
25	52484.0
26	79034.0
27	115421.0
28	164098.0
29	223670.0
30	301621.0
31	411776.0
32	567880.0
33	860485.0
34	1968392.0
35	4479120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50961659511993	17.40154070043478	12.02988506833641	26.058957636108882
2	15.730289315713275	19.36898587337402	39.711675090286036	25.18904972062667
3	19.17074066817416	23.57201427604809	28.33324713568061	28.92399792009714
4	12.60842607403599	15.860089795409504	36.446996900709735	35.08448722984477
5	14.245020113735544	36.74824276385632	34.56369635688993	14.443040765518205
6	33.75417603510793	35.81097558424259	17.360440042172602	13.074408338476879
7	30.121836592133068	30.37897219358544	21.00463234608583	18.49455886819566
8	26.964693426876803	35.20697790913135	19.305668043776407	18.522660620215436
9	27.113920139388075	15.273883833257873	18.72223664644653	38.88995938090753
10	15.045010480952218	28.029278431125153	33.06135857774047	23.864352510182155
11	36.111356982712344	21.805481481147694	23.260433387053148	18.822728149086814
12	24.520611819547522	23.7180390892102	30.00314574520794	21.758203346034332
13	29.329301295068028	20.811062481141402	25.32864931868683	24.530986905103745
14	22.63908592873346	20.1582723222151	26.24990262756433	30.95273912148711
15	24.15056958146416	28.766640671807046	22.568503180068827	24.51428656665997
16	24.326548323225904	26.945932121714932	25.055755484263408	23.67176407079576
17	23.288953217539614	27.023699262985534	26.06922147702717	23.61812604244768
18	23.450010906368	26.58827672196959	27.151738425704135	22.809973945958276
19	24.82540536138507	25.77185213288774	26.073342999267062	23.329399506460128
20	25.084193436109338	25.77921041213261	26.099342889919576	23.037253261838476
21	25.03094094927208	25.41913116848797	25.557047063396787	23.992880818843172
22	24.899756794011385	25.846862093395522	25.8295768396054	23.4238042729877
23	24.02376299750168	25.97765333157079	26.04152762678613	23.957056044141396
24	24.174612360075624	25.724710654935308	26.325113534559968	23.7755634504291
25	24.130652730341094	25.800440748493397	26.19151731376273	23.87738920740278
26	23.599284901046143	26.34240405880895	26.467310298648243	23.591000741496664
27	23.954715403659822	26.286134768369884	25.89987534203686	23.859274485933433
28	23.82188198246287	26.1221054212747	25.95796775292442	24.09804484333801
29	23.987766174442317	26.067965029903213	25.857143562251885	24.087125233402585
30	23.87106977181333	26.09906589604852	26.060419830174038	23.969444501964116
31	24.435127328238902	25.601899607327795	25.85731143032552	24.105661634107783
32	23.876085835292624	26.283370112151882	25.917162734550132	23.92338131800536
33	23.722354835155038	26.170976011752867	26.220493503230006	23.886175649862086
34	24.066461456846653	26.033591474797056	26.313186009607527	23.58676105874876
35	23.91957464160014	26.209492231092796	26.318267662460297	23.55266546484677
36	23.654796636633467	26.114827928505335	26.460388398207474	23.769987036653724
37	24.060415797997685	25.845022228984398	25.852050266239218	24.242511706778696
38	23.764421515486433	26.352255302977223	25.953261248242924	23.930061933293413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	152.5
2	251.0
3	251.0
4	742.0
5	1233.0
6	1233.0
7	1584.5
8	1936.0
9	1898.0
10	1860.0
11	1860.0
12	2846.5
13	3833.0
14	6494.0
15	9155.0
16	9155.0
17	15422.0
18	21689.0
19	21689.0
20	32301.5
21	42914.0
22	55728.0
23	68542.0
24	68542.0
25	93155.5
26	117769.0
27	117769.0
28	154418.0
29	191067.0
30	242571.0
31	294075.0
32	294075.0
33	359313.5
34	424552.0
35	424552.0
36	475349.5
37	526147.0
38	578246.5
39	630346.0
40	630346.0
41	667928.0
42	705510.0
43	740757.0
44	776004.0
45	776004.0
46	807184.0
47	838364.0
48	838364.0
49	853633.5
50	868903.0
51	858132.5
52	847362.0
53	847362.0
54	812950.0
55	778538.0
56	778538.0
57	721921.5
58	665305.0
59	594026.5
60	522748.0
61	522748.0
62	452663.0
63	382578.0
64	315782.5
65	248987.0
66	248987.0
67	200858.0
68	152729.0
69	152729.0
70	119414.0
71	86099.0
72	66972.5
73	47846.0
74	47846.0
75	36961.5
76	26077.0
77	26077.0
78	21052.5
79	16028.0
80	12210.0
81	8392.0
82	8392.0
83	6708.0
84	5024.0
85	5024.0
86	3569.0
87	2114.0
88	1683.5
89	1253.0
90	1253.0
91	984.5
92	716.0
93	651.5
94	587.0
95	587.0
96	562.5
97	538.0
98	538.0
99	2090.5
100	3643.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12054800634454158
2	0.054684335024753325
3	0.011823060074019651
4	0.0026499962234871636
5	2.467607819441488E-4
6	2.6821824124364E-4
7	5.3643648248728E-5
8	4.1842045634007843E-4
9	3.004044301928768E-4
10	5.3643648248728E-4
11	0.0019848149852029363
12	0.002596352575238435
13	0.015148966265440787
14	0.00946273955107562
15	0.02423620027877531
16	0.011340267239781099
17	0.02097466646525265
18	0.00563258306611644
19	0.008057275966958946
20	0.006051003522456519
21	0.0063835941415986315
22	0.0073491798100757365
23	0.009655856684771041
24	0.01458034359400427
25	0.01739127076223762
26	0.019075681317247677
27	0.00929107987667969
28	0.005825700199811861
29	0.01109350645783695
30	0.0030576879501774965
31	0.005643311795766186
32	0.006855658246187438
33	0.008894116879639102
34	0.012885204309344466
35	0.016822648090801102
36	0.015181152454390025
37	0.010235208085857303
38	0.006211934467202702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	9320768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.59149601714429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33627356009521	46.48430956311388
2	11.927133611519533	13.97657202990589
3	3.5705070820300633	6.276040544278498
4	1.5871037038637976	3.7196312137492256
5	0.8712263015794959	2.552322618951319
6	0.5561921053764671	1.9552876516159425
7	0.3812751442800529	1.5637636768264584
8	0.2768806848345792	1.2978282834167543
9	0.20269013962173252	1.0688326657525045
>10	1.17591282787656	12.578031842637975
>50	0.07636822395483885	3.0791382297216825
>100	0.03587822263126187	3.7472015027806873
>500	0.0018041281241280853	0.7505252468669457
>1k	6.99074188825052E-4	0.7209461996214567
>5k	3.679334892728836E-5	0.11442866211649311
>10k+	1.839667446364418E-5	0.11514006864434621
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	10682	0.1146042901185825	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.502022150964384E-4
2	0.0	0.0	0.0	0.0	1.502022150964384E-4
3	0.0	0.0	0.0	1.07287296497456E-5	1.502022150964384E-4
4	0.0	0.0	0.0	2.14574592994912E-5	1.502022150964384E-4
5	0.0	0.0	0.0	4.29149185989824E-5	1.716596743959296E-4
6	1.07287296497456E-5	0.0	0.0	4.29149185989824E-5	2.253033226446576E-4
7	1.07287296497456E-5	0.0	0.0	5.3643648248728E-5	2.253033226446576E-4
8	1.07287296497456E-5	0.0	0.0	5.3643648248728E-5	2.253033226446576E-4
9	1.07287296497456E-5	0.0	0.0	8.58298371979648E-5	2.253033226446576E-4
10	3.2186188949236804E-5	0.0	0.0	9.65585668477104E-5	2.3603205229440322E-4
11	3.2186188949236804E-5	0.0	0.0	1.1801602614720161E-4	2.3603205229440322E-4
12	3.2186188949236804E-5	1.07287296497456E-5	0.0	1.1801602614720161E-4	5.471652121370256E-4
13	3.2186188949236804E-5	1.07287296497456E-5	0.0	1.1801602614720161E-4	6.544525086344817E-4
14	3.2186188949236804E-5	1.07287296497456E-5	0.0	1.2874475579694722E-4	6.759099679339728E-4
15	4.29149185989824E-5	1.07287296497456E-5	0.0	1.931171336954208E-4	8.475696423299024E-4
16	6.437237789847361E-5	1.07287296497456E-5	0.0	2.467607819441488E-4	8.904845609288848E-4
17	6.437237789847361E-5	1.07287296497456E-5	0.0	2.789469708933856E-4	9.012132905786304E-4
18	6.437237789847361E-5	1.07287296497456E-5	0.0	3.1113315984262243E-4	0.0010085005870760864
19	6.437237789847361E-5	1.07287296497456E-5	0.0	3.6477680809135043E-4	0.001019229316725832
20	7.51011075482192E-5	1.07287296497456E-5	0.0	4.613353749390608E-4	0.0010943304242740511
21	7.51011075482192E-5	1.07287296497456E-5	0.0	5.686226714365168E-4	0.0011372453428730336
22	7.51011075482192E-5	1.07287296497456E-5	0.0	8.261121830304112E-4	0.0012230751800709984
23	7.51011075482192E-5	1.07287296497456E-5	0.0	0.0010943304242740511	0.0012767188283197264
24	8.58298371979648E-5	1.07287296497456E-5	0.0	0.0013625486655176912	0.001287447557969472
25	9.65585668477104E-5	1.07287296497456E-5	0.0	0.001748782932908533	0.001287447557969472
26	9.65585668477104E-5	1.07287296497456E-5	0.0	0.002253033226446576	0.0013089050172689634
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9065	0.0	22.27654	1
GGTATCA	2890	0.0	21.167328	1
TCGCGTA	300	0.0	17.067053	9
CGCGTAA	315	0.0	16.762285	10
GTCCTAC	5025	0.0	16.093697	1
TAGGACG	4555	0.0	15.733279	4
GTAGGAC	4950	0.0	15.7071705	3
TATCAAC	12740	0.0	15.56338	2
ATCAACG	12940	0.0	15.157343	3
TCAACGC	12930	0.0	15.155393	4
TAGGACC	2805	0.0	15.05569	4
CAACGCA	13310	0.0	14.734408	5
AGGACGT	4705	0.0	14.449392	5
TCCTACA	5975	0.0	14.409418	2
AACGCAG	13780	0.0	14.266758	6
TTAGGAC	3020	0.0	14.249913	3
GCGTAAC	360	0.0	14.222697	11
GGACGTG	4750	0.0	14.043168	6
TGTAGGA	5800	0.0	13.988851	2
GTCCTAA	2775	0.0	13.907676	1
>>END_MODULE