FastQCFastQC Report
Thu 2 Feb 2017
SRR4062730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320768
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT96490.1035215123903953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88700.022.080651
GGTATCA31100.020.2202341
GTAGGAC53000.016.0909653
TAGGACC28650.015.7493354
TAGGACG47000.015.3538194
ATACCGT6850.015.1834296
TGTAGGA61050.015.0957122
GTCCTAC44850.014.9844531
TATCAAC130950.014.88190652
AGGACGT48700.014.5224665
ATCAACG135400.014.2396453
TCAACGC135800.014.1979294
GGACGTG50200.013.9929056
TTAGGAC33600.013.8574873
CAACGCA139000.013.8368345
ACCGTCG5450.013.7989518
CTGTAGG63850.013.7583241
AACGCAG143100.013.49636
TACCGTC6500.013.0469687
GAAAATC74000.012.99428931
Produced by FastQC (version 0.11.5)