##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9320768
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22810931459725	32.0	32.0	32.0	32.0	32.0
2	31.278652681839095	32.0	32.0	32.0	32.0	32.0
3	31.37207803047989	32.0	32.0	32.0	32.0	32.0
4	31.46836591147854	32.0	32.0	32.0	32.0	32.0
5	31.39523867561128	32.0	32.0	32.0	32.0	32.0
6	34.92501712305252	36.0	36.0	36.0	36.0	36.0
7	34.955614279853336	36.0	36.0	36.0	36.0	36.0
8	34.88480444959042	36.0	36.0	36.0	32.0	36.0
9	35.008630619279444	36.0	36.0	36.0	36.0	36.0
10	34.85810149979058	36.0	36.0	36.0	32.0	36.0
11	35.03270717606103	36.0	36.0	36.0	36.0	36.0
12	34.95157673702425	36.0	36.0	36.0	36.0	36.0
13	34.99643934920384	36.0	36.0	36.0	36.0	36.0
14	34.945092078249345	36.0	36.0	36.0	32.0	36.0
15	34.91691628844318	36.0	36.0	36.0	32.0	36.0
16	34.9409550800964	36.0	36.0	36.0	36.0	36.0
17	34.918623014755866	36.0	36.0	36.0	32.0	36.0
18	34.917815892424315	36.0	36.0	36.0	32.0	36.0
19	34.9069363168357	36.0	36.0	36.0	32.0	36.0
20	34.90083960892493	36.0	36.0	36.0	32.0	36.0
21	34.89432684087835	36.0	36.0	36.0	32.0	36.0
22	34.87349186247314	36.0	36.0	36.0	32.0	36.0
23	34.826830042331274	36.0	36.0	36.0	32.0	36.0
24	34.81410888029828	36.0	36.0	36.0	32.0	36.0
25	34.803724006433804	36.0	36.0	36.0	32.0	36.0
26	34.73833754900884	36.0	36.0	36.0	32.0	36.0
27	34.716974395242964	36.0	36.0	36.0	32.0	36.0
28	34.69442196179542	36.0	36.0	36.0	32.0	36.0
29	34.674115373325456	36.0	36.0	36.0	32.0	36.0
30	34.64955892046664	36.0	36.0	36.0	32.0	36.0
31	34.63846981278796	36.0	36.0	36.0	32.0	36.0
32	34.62020736917816	36.0	36.0	36.0	32.0	36.0
33	34.606603983706066	36.0	36.0	36.0	32.0	36.0
34	34.60224543728585	36.0	36.0	36.0	32.0	36.0
35	34.5842665540007	36.0	36.0	36.0	32.0	36.0
36	34.55695807469942	36.0	36.0	36.0	32.0	36.0
37	34.54465157806739	36.0	36.0	36.0	32.0	36.0
38	34.16094156618854	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	43.0
21	177.0
22	710.0
23	2061.0
24	5705.0
25	13860.0
26	29426.0
27	57583.0
28	101186.0
29	159611.0
30	242795.0
31	352776.0
32	509840.0
33	822598.0
34	2058062.0
35	4964329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.852167066562544	18.63116724317339	12.703319747388553	26.81334594287551
2	14.914716782427885	20.36461162477294	38.79902801990437	25.921643572894805
3	18.16291318483627	24.348723195341844	28.627919931061474	28.860443688760412
4	12.179947828210318	16.767327422075443	36.308295494802664	34.744429254911566
5	13.840611383227516	37.303264560015776	34.123622457639215	14.732501599117498
6	33.86953805034699	36.185702103992426	17.002880453044796	12.941879392615792
7	30.015043892503652	30.536537604474155	20.965094349946558	18.48332415307563
8	26.911221803279055	35.451110520063054	19.075487340168916	18.56218033648898
9	26.950480011690487	15.122937883124735	18.518876554413236	39.407705550771546
10	14.827677882867823	28.34231908302009	33.02038777411615	23.80961525999594
11	36.25303209514556	21.787202283877924	23.102494257688175	18.857271363288344
12	24.392733237139243	23.836285942563137	29.90401297579516	21.866967844502458
13	29.303498407961413	20.80416455578594	25.301976642662172	24.590360393590476
14	22.53156606837548	20.186137022185296	26.12073382794208	31.161563081497146
15	24.093937323619684	28.935062003474393	22.43461053853073	24.536390134375193
16	24.31527362501391	27.05383580557265	24.887984003891525	23.742906565521913
17	23.280088078578935	27.136551408639285	25.930019929688196	23.65334058309358
18	23.4733876006784	26.67837028021725	26.99383784683837	22.854404272265977
19	24.83318971140576	25.88185866229049	25.98168949168137	23.303262134622383
20	25.107287296497454	25.842334022260825	25.96016766000398	23.090211021237735
21	25.038948008418625	25.468945179249886	25.485853673504486	24.006253138827
22	24.860132158708467	25.9316538785505	25.734266604568745	23.473947358172286
23	23.97491875384693	26.042102410439334	25.90473147597902	24.078247359734718
24	24.241811404382126	25.850262553471985	26.127063778435428	23.78086226371046
25	24.10330014898998	25.884077491097564	26.0992637832898	23.91335857662266
26	23.60472811027079	26.40445568625061	26.289690415935862	23.701125787542736
27	24.00682733952732	26.35713972281877	25.753380008349104	23.882652929304808
28	23.80396121864636	26.1701074417902	25.90783291677252	24.11809842279091
29	24.007860725639777	26.076434903218278	25.7380507700653	24.17765360107665
30	23.834076762773197	26.136419230690006	25.978867835783486	24.05063617075331
31	24.372154740896885	25.651276804658156	25.819245796054574	24.15732265839038
32	23.923608011700324	26.303025673420905	25.796554532845363	23.976811782033412
33	23.72845241937145	26.218054134594915	26.114886670282967	23.938606775750667
34	24.070183916175147	26.078988340874915	26.210490380191846	23.64033736275809
35	23.93855313210242	26.237505321449905	26.18817462252038	23.6357669239273
36	23.72640323200835	26.14961556815919	26.303036402150553	23.82094479768191
37	24.056547700790322	25.898928071163237	25.753328481086534	24.291195746959907
38	23.81716726962179	26.326125766568392	25.87074444573189	23.985962518077926
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	56.5
2	96.0
3	96.0
4	492.5
5	889.0
6	889.0
7	1133.0
8	1377.0
9	1422.0
10	1467.0
11	1467.0
12	2440.5
13	3414.0
14	6039.5
15	8665.0
16	8665.0
17	15016.5
18	21368.0
19	21368.0
20	31515.0
21	41662.0
22	54996.5
23	68331.0
24	68331.0
25	93586.5
26	118842.0
27	118842.0
28	155147.0
29	191452.0
30	243203.5
31	294955.0
32	294955.0
33	359842.0
34	424729.0
35	424729.0
36	477175.5
37	529622.0
38	582115.5
39	634609.0
40	634609.0
41	672463.0
42	710317.0
43	744375.0
44	778433.0
45	778433.0
46	807549.5
47	836666.0
48	836666.0
49	854172.0
50	871678.0
51	859386.0
52	847094.0
53	847094.0
54	812417.0
55	777740.0
56	777740.0
57	722164.0
58	666588.0
59	594970.0
60	523352.0
61	523352.0
62	453141.5
63	382931.0
64	314941.5
65	246952.0
66	246952.0
67	199263.5
68	151575.0
69	151575.0
70	118247.5
71	84920.0
72	65721.0
73	46522.0
74	46522.0
75	35729.0
76	24936.0
77	24936.0
78	20093.5
79	15251.0
80	11399.5
81	7548.0
82	7548.0
83	5878.0
84	4208.0
85	4208.0
86	2871.0
87	1534.0
88	1096.5
89	659.0
90	659.0
91	415.5
92	172.0
93	139.0
94	106.0
95	106.0
96	88.5
97	71.0
98	71.0
99	45.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009033590365085796
2	1.0728729649745601E-5
3	0.0
4	2.360320522944032E-4
5	2.1457459299491203E-5
6	1.287447557969472E-4
7	4.506066452893152E-4
8	0.0024139641711927603
9	0.0039696299704058725
10	9.012132905786304E-4
11	0.005149790231877888
12	3.1113315984262243E-4
13	1.502022150964384E-4
14	0.0
15	0.0
16	1.0728729649745601E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	9.65585668477104E-5
22	5.3643648248728E-5
23	2.038458633451664E-4
24	0.0
25	7.51011075482192E-5
26	4.2914918598982405E-5
27	7.51011075482192E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.582983719796481E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	9320768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.84543191102755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.12145755545565	44.40847996258883
2	12.541687699264143	14.258753083157833
3	3.7116152842526215	6.3296512176273465
4	1.7338189675498075	3.942387522636027
5	0.9117657936725193	2.591486017150759
6	0.6055856429967148	2.0654866461159354
7	0.4187273137195648	1.6661914500933093
8	0.3114169311770272	1.4162103965731883
9	0.23099351593546327	1.1817833563798412
>10	1.2885639545629535	13.276073867148849
>50	0.08381934240380028	3.2603269983538694
>100	0.03775089991331719	3.8416762713550567
>500	0.0019031901913469965	0.7659161288321945
>1k	8.368509648417979E-4	0.7709766775418964
>5k	5.7057940371070495E-5	0.22460040444515955
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9649	0.1035215123903953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.394734854466928E-4
2	0.0	0.0	0.0	0.0	1.394734854466928E-4
3	0.0	1.07287296497456E-5	0.0	0.0	1.502022150964384E-4
4	0.0	1.07287296497456E-5	0.0	3.2186188949236804E-5	1.502022150964384E-4
5	2.14574592994912E-5	1.07287296497456E-5	0.0	5.3643648248728E-5	1.502022150964384E-4
6	3.2186188949236804E-5	1.07287296497456E-5	0.0	5.3643648248728E-5	1.8238840404567522E-4
7	3.2186188949236804E-5	1.07287296497456E-5	0.0	5.3643648248728E-5	1.8238840404567522E-4
8	3.2186188949236804E-5	1.07287296497456E-5	0.0	5.3643648248728E-5	1.8238840404567522E-4
9	6.437237789847361E-5	1.07287296497456E-5	0.0	7.51011075482192E-5	2.038458633451664E-4
10	7.51011075482192E-5	1.07287296497456E-5	0.0	8.58298371979648E-5	2.14574592994912E-4
11	7.51011075482192E-5	1.07287296497456E-5	0.0	9.65585668477104E-5	2.253033226446576E-4
12	7.51011075482192E-5	1.07287296497456E-5	0.0	9.65585668477104E-5	6.544525086344817E-4
13	7.51011075482192E-5	1.07287296497456E-5	0.0	9.65585668477104E-5	7.188248865329552E-4
14	7.51011075482192E-5	1.07287296497456E-5	0.0	9.65585668477104E-5	7.617398051319377E-4
15	7.51011075482192E-5	1.07287296497456E-5	0.0	1.716596743959296E-4	8.904845609288848E-4
16	1.07287296497456E-4	1.07287296497456E-5	0.0	2.253033226446576E-4	9.763143981268497E-4
17	1.07287296497456E-4	1.07287296497456E-5	0.0	2.5748951159389443E-4	0.0010085005870760864
18	1.07287296497456E-4	1.07287296497456E-5	0.0	3.1113315984262243E-4	0.0010621442353248144
19	1.07287296497456E-4	1.07287296497456E-5	0.0	3.6477680809135043E-4	0.0010836016946243056
20	1.07287296497456E-4	1.07287296497456E-5	0.0	4.076917266903328E-4	0.0012016177207715072
21	1.07287296497456E-4	1.07287296497456E-5	0.0	5.042502935380432E-4	0.0012552613690202353
22	1.07287296497456E-4	1.07287296497456E-5	0.0	7.080961568832096E-4	0.0013410912062182
23	1.07287296497456E-4	1.07287296497456E-5	0.0	9.333994795278672E-4	0.0013518199358679456
24	1.07287296497456E-4	1.07287296497456E-5	0.0	0.001180160261472016	0.0013840061248171825
25	1.1801602614720161E-4	1.07287296497456E-5	0.0	0.001534208339913621	0.0013840061248171825
26	1.1801602614720161E-4	1.07287296497456E-5	0.0	0.0019097138776547169	0.0015234796102638754
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8870	0.0	22.08065	1
GGTATCA	3110	0.0	20.220234	1
GTAGGAC	5300	0.0	16.090965	3
TAGGACC	2865	0.0	15.749335	4
TAGGACG	4700	0.0	15.353819	4
ATACCGT	685	0.0	15.183429	6
TGTAGGA	6105	0.0	15.095712	2
GTCCTAC	4485	0.0	14.984453	1
TATCAAC	13095	0.0	14.8819065	2
AGGACGT	4870	0.0	14.522466	5
ATCAACG	13540	0.0	14.239645	3
TCAACGC	13580	0.0	14.197929	4
GGACGTG	5020	0.0	13.992905	6
TTAGGAC	3360	0.0	13.857487	3
CAACGCA	13900	0.0	13.836834	5
ACCGTCG	545	0.0	13.798951	8
CTGTAGG	6385	0.0	13.758324	1
AACGCAG	14310	0.0	13.4963	6
TACCGTC	650	0.0	13.046968	7
GAAAATC	7400	0.0	12.994289	31
>>END_MODULE