FastQCFastQC Report
Thu 2 Feb 2017
SRR4062729_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062729_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459326
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT77730.12033763274991849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64900.022.3593481
GGTATCA20750.018.9885861
GCGTACG652.0855104E-417.2488561
TCAACGC93850.015.1529844
TATCAAC95150.015.1226212
CAACGCA96250.014.8416235
AACGCAG98650.014.6102746
ATCAACG98250.014.6062133
ACCGTCG9300.014.2785128
TAATACT12200.013.767664
ATACCGT10500.013.5591166
ATCGTTT9750.013.45614229
TACCGTC10150.013.3978167
CGTCGTA10450.013.01459910
CATCGTT10000.012.95954228
CTAGCGG11550.012.88286129
TCTAGCG11800.012.88090128
AGGACCT42900.012.789915
TAGGACC34650.012.6958024
GTATTAG15800.012.6715161
Produced by FastQC (version 0.11.5)