##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062729_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6459326
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300279007438238	32.0	32.0	32.0	32.0	32.0
2	30.942787374410273	32.0	32.0	32.0	32.0	32.0
3	30.981359200634866	32.0	32.0	32.0	32.0	32.0
4	31.050252611495377	32.0	32.0	32.0	32.0	32.0
5	30.965697195032423	32.0	32.0	32.0	32.0	32.0
6	34.616858167554945	36.0	36.0	36.0	32.0	36.0
7	34.56707402598971	36.0	36.0	36.0	32.0	36.0
8	34.560568703298145	36.0	36.0	36.0	32.0	36.0
9	34.69650703494451	36.0	36.0	36.0	32.0	36.0
10	34.43152768570591	36.0	36.0	36.0	32.0	36.0
11	34.665019694005224	36.0	36.0	36.0	32.0	36.0
12	34.50672361172048	36.0	36.0	36.0	32.0	36.0
13	34.57899369067299	36.0	36.0	36.0	32.0	36.0
14	34.49248683221748	36.0	36.0	36.0	32.0	36.0
15	34.43735166796041	36.0	36.0	36.0	32.0	36.0
16	34.45767747285088	36.0	36.0	36.0	32.0	36.0
17	34.40233454697905	36.0	36.0	36.0	32.0	36.0
18	34.426155608185745	36.0	36.0	36.0	32.0	36.0
19	34.404697022568605	36.0	36.0	36.0	32.0	36.0
20	34.38952949580188	36.0	36.0	36.0	32.0	36.0
21	34.37293287256286	36.0	36.0	36.0	32.0	36.0
22	34.35454333780336	36.0	36.0	36.0	32.0	36.0
23	34.30118405542622	36.0	36.0	36.0	32.0	36.0
24	34.28205775649038	36.0	36.0	36.0	32.0	36.0
25	34.263425471945524	36.0	36.0	36.0	32.0	36.0
26	34.22293115411732	36.0	36.0	36.0	32.0	36.0
27	34.2190960790646	36.0	36.0	36.0	32.0	36.0
28	34.19802886555037	36.0	36.0	36.0	32.0	36.0
29	34.16469318935133	36.0	36.0	36.0	32.0	36.0
30	34.134404270662294	36.0	36.0	36.0	32.0	36.0
31	34.15218042873204	36.0	36.0	36.0	32.0	36.0
32	34.106969364915166	36.0	36.0	36.0	32.0	36.0
33	34.090339920914346	36.0	36.0	36.0	32.0	36.0
34	34.08788796230443	36.0	36.0	36.0	32.0	36.0
35	34.06049841732713	36.0	36.0	36.0	32.0	36.0
36	34.021229149914404	36.0	36.0	36.0	32.0	36.0
37	34.01033652737143	36.0	36.0	36.0	32.0	36.0
38	33.420419715617385	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	11.0
8	9.0
9	28.0
10	47.0
11	67.0
12	25.0
13	31.0
14	371.0
15	1060.0
16	1633.0
17	1842.0
18	2291.0
19	3220.0
20	4201.0
21	6223.0
22	9635.0
23	15074.0
24	23161.0
25	36333.0
26	53983.0
27	79599.0
28	114087.0
29	157077.0
30	215638.0
31	294298.0
32	408927.0
33	619420.0
34	1407660.0
35	3003369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.394059318157545	18.394683199337088	12.280756140889576	25.93050134161579
2	15.72390592209495	20.181927569753256	39.63888969781386	24.455276810337935
3	18.419992837404912	24.616318856561215	29.45413624383047	27.509552062203408
4	12.405124591876088	16.609871083237383	37.541284591157726	33.443719733728805
5	13.943946352181161	36.913265685261834	34.4906547322687	14.652133230288303
6	32.03188087370512	36.6616888280085	18.198577677815045	13.107852620471332
7	28.858298484485374	31.12526945544147	21.994174622607567	18.02225743746559
8	26.432422574451543	34.700916600586254	20.288600842629744	18.578059982332455
9	26.97390696125545	15.414775791032756	19.601864533806946	38.00945271390484
10	15.39922164868173	27.240562203856953	32.83329416496601	24.5269219824953
11	35.53485706341334	21.558544542638685	24.524611665884578	18.3819867280634
12	24.709853733212203	23.82034123343669	30.109150483437773	21.36065454991333
13	28.62526121117755	20.689566541186206	26.00156757674065	24.683604670895594
14	22.745608066000745	21.11423092674007	26.08449203905111	30.05566896820807
15	24.066134182335105	29.040753943305592	22.767731325625835	24.125380548733467
16	24.451246796881655	26.572450472629306	25.34907990183555	23.62722282865349
17	22.965055915892265	26.943731676804735	26.417129258069405	23.674083149233592
18	23.54733759325019	25.820316806028455	27.912137047280616	22.720208553440735
19	24.95985332296198	25.481945917022564	26.180262760233454	23.377937999782002
20	24.871697213474654	25.833851994290086	25.931948585990888	23.36250220624437
21	25.393692528043815	25.23916183393764	25.300379584112555	24.06676605390599
22	24.73333887611314	25.984443367885905	25.717120361773212	23.565097394227738
23	23.34595974257918	25.79054405037558	26.305215380530246	24.558280826514995
24	24.168366784467178	25.8732845532685	26.26929742089962	23.6890512413647
25	24.089197526371876	25.577505579722914	26.527119141136097	23.806177752769113
26	23.389572784565118	26.387962403802977	26.898964091605894	23.323500720026015
27	23.982028098630245	26.216810716750256	25.843317335911337	23.957843848708162
28	23.329944752612963	26.290163661326893	26.145774822773	24.234116763287147
29	23.888625589848772	25.882990663524417	25.803515813760992	24.424867932865816
30	23.554562646546326	26.09332364163313	26.41794915263421	23.93416455918633
31	24.51308679392979	25.429581941596112	25.704038146114684	24.353293118359414
32	23.65966375300497	26.18273159615989	25.818365187912523	24.339239462922613
33	23.386935952451584	25.87635962546394	26.55686134374936	24.17984307833511
34	24.02531668304816	25.894989988399796	26.68271061985214	23.396982708699905
35	24.392810485954453	25.96040499585104	26.334500782643687	23.31228373555082
36	23.559988678226272	26.092523293868158	26.210571984927423	24.136916042978147
37	24.230370483683075	26.027607447452212	25.560330172115343	24.18169189674937
38	23.73884777861297	26.39190947705613	25.951293677033995	23.917949067296906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	102.0
1	237.5
2	373.0
3	373.0
4	971.5
5	1570.0
6	1570.0
7	1690.0
8	1810.0
9	1902.5
10	1995.0
11	1995.0
12	2903.5
13	3812.0
14	6073.5
15	8335.0
16	8335.0
17	13924.5
18	19514.0
19	19514.0
20	27730.5
21	35947.0
22	47885.0
23	59823.0
24	59823.0
25	79940.5
26	100058.0
27	100058.0
28	130175.5
29	160293.0
30	199012.0
31	237731.0
32	237731.0
33	289258.5
34	340786.0
35	340786.0
36	368144.5
37	395503.0
38	425787.0
39	456071.0
40	456071.0
41	462486.0
42	468901.0
43	491144.5
44	513388.0
45	513388.0
46	512525.5
47	511663.0
48	511663.0
49	530954.5
50	550246.0
51	547021.5
52	543797.0
53	543797.0
54	517618.0
55	491439.0
56	491439.0
57	465115.5
58	438792.0
59	391584.0
60	344376.0
61	344376.0
62	316286.0
63	288196.0
64	237842.0
65	187488.0
66	187488.0
67	153870.0
68	120252.0
69	120252.0
70	95412.5
71	70573.0
72	54780.5
73	38988.0
74	38988.0
75	29206.0
76	19424.0
77	19424.0
78	18344.0
79	17264.0
80	13630.0
81	9996.0
82	9996.0
83	9822.0
84	9648.0
85	9648.0
86	6522.5
87	3397.0
88	2854.0
89	2311.0
90	2311.0
91	1692.0
92	1073.0
93	962.5
94	852.0
95	852.0
96	793.0
97	734.0
98	734.0
99	1769.5
100	2805.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12039955871556877
2	0.05526892434288036
3	0.011967192861917792
4	0.003049853808276591
5	2.1674087977600141E-4
6	4.6444474237714587E-4
7	3.096298282514306E-5
8	3.715557939017167E-4
9	2.63185354013716E-4
10	4.95407725202289E-4
11	0.0020590383578720133
12	0.00263185354013716
13	0.014707416841942952
14	0.009459191253081204
15	0.023934385723835584
16	0.012013637336155505
17	0.020482013138832134
18	0.005542373925700607
19	0.00789556062041148
20	0.00597585568525261
21	0.006424818936217184
22	0.007322745438146333
23	0.00992363599545835
24	0.014707416841942952
25	0.01733927038208011
26	0.018980308471812694
27	0.008932820545053772
28	0.005852003753952038
29	0.010511932669136068
30	0.002956964859801162
31	0.005759114805476608
32	0.006858300695769187
33	0.008700598173865199
34	0.012663859975483511
35	0.01682838116546525
36	0.01507897263584467
37	0.009815265555570349
38	0.006362892970566899
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	6459326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.01575097387119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.67838887122093	45.19951015162624
2	9.461295933827483	10.22118010103451
3	2.6202308779884382	4.246012157984142
4	1.198726091566295	2.590003601917076
5	0.6716870535911839	1.8140840309577335
6	0.42468178324395783	1.37637032681071
7	0.2991542966422465	1.1311330793133825
8	0.22227772052421235	0.9605198399100475
9	0.1745247826627505	0.8484378479172112
>10	1.0804784525823699	11.04463352483823
>50	0.08874353721486093	3.297130622841203
>100	0.06313893886885472	6.939686886482025
>500	0.010077897939322138	3.7966752005105553
>1k	0.006564968406188323	6.413728034339074
>5k	2.879372107088577E-5	0.1208945935180502
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7773	0.12033763274991849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.644447423771459E-5
2	0.0	0.0	0.0	0.0	4.644447423771459E-5
3	0.0	0.0	0.0	0.0	4.644447423771459E-5
4	0.0	0.0	0.0	0.0	4.644447423771459E-5
5	0.0	0.0	0.0	0.0	4.644447423771459E-5
6	0.0	0.0	0.0	0.0	7.740745706285764E-5
7	0.0	0.0	0.0	7.740745706285764E-5	7.740745706285764E-5
8	0.0	0.0	0.0	7.740745706285764E-5	7.740745706285764E-5
9	0.0	0.0	0.0	1.083704398880007E-4	9.288894847542917E-5
10	0.0	0.0	0.0	1.2385193130057221E-4	1.083704398880007E-4
11	1.5481491412571527E-5	0.0	0.0	1.3933342271314376E-4	1.083704398880007E-4
12	1.5481491412571527E-5	0.0	0.0	1.3933342271314376E-4	2.63185354013716E-4
13	1.5481491412571527E-5	0.0	0.0	1.3933342271314376E-4	3.405928110765736E-4
14	1.5481491412571527E-5	0.0	0.0	1.3933342271314376E-4	3.405928110765736E-4
15	1.5481491412571527E-5	0.0	0.0	1.5481491412571528E-4	4.0251877672685973E-4
16	1.5481491412571527E-5	0.0	0.0	1.702964055382868E-4	4.6444474237714587E-4
17	1.5481491412571527E-5	0.0	0.0	2.0125938836342987E-4	4.6444474237714587E-4
18	1.5481491412571527E-5	0.0	0.0	2.786668454262875E-4	4.954077252022889E-4
19	1.5481491412571527E-5	0.0	0.0	3.251113196640021E-4	4.954077252022889E-4
20	1.5481491412571527E-5	0.0	0.0	3.560743024891452E-4	5.57333690852575E-4
21	1.5481491412571527E-5	0.0	0.0	4.6444474237714587E-4	5.57333690852575E-4
22	1.5481491412571527E-5	0.0	0.0	6.192596565028611E-4	5.57333690852575E-4
23	1.5481491412571527E-5	0.0	0.0	8.824450105165771E-4	5.57333690852575E-4
24	1.5481491412571527E-5	0.0	0.0	0.0012075563301805793	5.57333690852575E-4
25	1.5481491412571527E-5	1.5481491412571527E-5	0.0	0.0015791121240822958	5.57333690852575E-4
26	1.5481491412571527E-5	3.0962982825143053E-5	0.0	0.0019816309008091554	5.728151822651465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6490	0.0	22.359348	1
GGTATCA	2075	0.0	18.988586	1
GCGTACG	65	2.0855104E-4	17.248856	1
TCAACGC	9385	0.0	15.152984	4
TATCAAC	9515	0.0	15.122621	2
CAACGCA	9625	0.0	14.841623	5
AACGCAG	9865	0.0	14.610274	6
ATCAACG	9825	0.0	14.606213	3
ACCGTCG	930	0.0	14.278512	8
TAATACT	1220	0.0	13.76766	4
ATACCGT	1050	0.0	13.559116	6
ATCGTTT	975	0.0	13.456142	29
TACCGTC	1015	0.0	13.397816	7
CGTCGTA	1045	0.0	13.014599	10
CATCGTT	1000	0.0	12.959542	28
CTAGCGG	1155	0.0	12.882861	29
TCTAGCG	1180	0.0	12.880901	28
AGGACCT	4290	0.0	12.78991	5
TAGGACC	3465	0.0	12.695802	4
GTATTAG	1580	0.0	12.671516	1
>>END_MODULE