FastQCFastQC Report
Thu 2 Feb 2017
SRR4062729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459326
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT71420.11056881166858586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61350.021.8817311
GGTATCA20600.018.8744071
ACCGTCG10150.014.3465218
CTAGCGG10200.014.27398629
TCAACGC92650.014.1275124
TCTAGCG10500.014.01853228
TATCAAC95300.013.9180732
TACCGTC10650.013.8232287
CAACGCA95450.013.6798745
ATACCGT11000.013.6743416
CGTCGTA10700.013.6078210
TAGGACC33400.013.5580684
TAGCGGC11050.013.46556830
AACGCAG97700.013.3976926
ATCAACG98250.013.321973
AACGAAT10500.012.95190431
CCGTCGT10800.012.7419499
GTAGGAC50200.012.5266973
TGTAGGA50750.012.3270222
GTCGTAG11400.012.2105511
Produced by FastQC (version 0.11.5)