##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062729_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6459326
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195630163270906	32.0	32.0	32.0	32.0	32.0
2	31.215720030232255	32.0	32.0	32.0	32.0	32.0
3	31.321841473862754	32.0	32.0	32.0	32.0	32.0
4	31.434644729186914	32.0	32.0	32.0	32.0	32.0
5	31.346736021683995	32.0	32.0	32.0	32.0	32.0
6	34.858804618314664	36.0	36.0	36.0	32.0	36.0
7	34.88878514569477	36.0	36.0	36.0	36.0	36.0
8	34.8155795202162	36.0	36.0	36.0	32.0	36.0
9	34.95990371131601	36.0	36.0	36.0	32.0	36.0
10	34.77741470240084	36.0	36.0	36.0	32.0	36.0
11	34.97639242855988	36.0	36.0	36.0	36.0	36.0
12	34.87110559213144	36.0	36.0	36.0	32.0	36.0
13	34.93124019441038	36.0	36.0	36.0	36.0	36.0
14	34.880526079655986	36.0	36.0	36.0	32.0	36.0
15	34.8380241220214	36.0	36.0	36.0	32.0	36.0
16	34.86443786859496	36.0	36.0	36.0	32.0	36.0
17	34.84109534028783	36.0	36.0	36.0	32.0	36.0
18	34.841007405416605	36.0	36.0	36.0	32.0	36.0
19	34.828669430835355	36.0	36.0	36.0	32.0	36.0
20	34.82088533695311	36.0	36.0	36.0	32.0	36.0
21	34.811235104096	36.0	36.0	36.0	32.0	36.0
22	34.79263656920242	36.0	36.0	36.0	32.0	36.0
23	34.736606110296954	36.0	36.0	36.0	32.0	36.0
24	34.72063215264255	36.0	36.0	36.0	32.0	36.0
25	34.705107003424196	36.0	36.0	36.0	32.0	36.0
26	34.63958762880214	36.0	36.0	36.0	32.0	36.0
27	34.61567414309171	36.0	36.0	36.0	32.0	36.0
28	34.59067323742446	36.0	36.0	36.0	32.0	36.0
29	34.55812448543393	36.0	36.0	36.0	32.0	36.0
30	34.53087767980746	36.0	36.0	36.0	32.0	36.0
31	34.52543593557594	36.0	36.0	36.0	32.0	36.0
32	34.5052420329923	36.0	36.0	36.0	32.0	36.0
33	34.483500755341964	36.0	36.0	36.0	32.0	36.0
34	34.47232528595089	36.0	36.0	36.0	32.0	36.0
35	34.453962534171524	36.0	36.0	36.0	32.0	36.0
36	34.4218073216927	36.0	36.0	36.0	32.0	36.0
37	34.40987976144879	36.0	36.0	36.0	32.0	36.0
38	33.98342149010593	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	35.0
21	125.0
22	446.0
23	1458.0
24	4021.0
25	9886.0
26	21267.0
27	41640.0
28	74038.0
29	118095.0
30	182447.0
31	267094.0
32	388827.0
33	628250.0
34	1526895.0
35	3194799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60983409697167	19.75830393791514	13.027319585314423	26.60454237979876
2	14.759916864378244	21.31917808749366	38.733876991790936	25.18702805633716
3	17.42096621226425	25.51424095950568	29.722481881236522	27.342310946993543
4	11.921304287919297	17.6698439926246	37.50693185495045	32.90191986450565
5	13.508335161440293	37.55526701482493	33.99980152718791	14.936596296546867
6	32.08259202677187	37.1184812757264	17.80477994883669	12.994146748665028
7	28.724720352694728	31.388709947847264	21.884084289668053	18.00248540978995
8	26.3326834559317	35.02539878594252	20.00566017258882	18.63625758553696
9	26.77469741466557	15.32473552376675	19.367752748956622	38.53281431261105
10	15.157596152997641	27.59347312533953	32.73614961954638	24.51278110211645
11	35.58384752557595	21.54852811535164	24.434610445768833	18.43301391330358
12	24.61394768746363	23.937526908904736	29.95727241355981	21.491252990071825
13	28.603749562260017	20.68441014014801	25.991212069888537	24.720628227703436
14	22.618404031133906	21.107224223463632	25.981310118520142	30.293061626882317
15	24.00107998884094	29.182038497515066	22.60910813295381	24.207773380690185
16	24.39004453097569	26.77066516558079	25.13413168312969	23.705158620313828
17	22.981020001158015	27.078351518409193	26.22434600761751	23.716282472815276
18	23.561544966146624	25.90869387920659	27.73284704936707	22.796914105279715
19	24.958052899017638	25.62038206463027	26.04137026061233	23.38019477573976
20	24.90275301169193	25.939951010368574	25.749513184502533	23.40778279343696
21	25.358071969230178	25.332991914314146	25.28379165843566	24.025144458020016
22	24.72641099904572	26.012861407788186	25.66095151752722	23.599776075638875
23	23.32396545822471	25.81062463492612	26.19840605241211	24.667003854437063
24	24.177909583755334	25.993826600484322	26.07171398378097	23.756549831979374
25	24.05837268085216	25.728145194163538	26.317386512487456	23.89609561249685
26	23.416745596519263	26.474837451980253	26.683636455962407	23.424780495538077
27	24.04226760711654	26.299765300372176	25.641631007598324	24.01633608491296
28	23.31223102843857	26.372720621315597	26.090105995579105	24.224942354666727
29	23.895635550829915	25.807444925368372	25.76869475236271	24.528224771439003
30	23.575772456754777	26.033521144466153	26.333800151904395	24.056906246874675
31	24.444144791577326	25.472843451468464	25.721321388640238	24.361690368313972
32	23.696125570996106	26.190782134235057	25.75496266947976	24.35812962528908
33	23.39445322933074	25.887871273256685	26.464262680038136	24.253412817374446
34	24.00728806689738	25.92451596343024	26.569753562523395	23.498442407148982
35	24.42691389163513	26.03257677348999	26.097382296543014	23.443127038331863
36	23.57055519414874	26.136271802971393	26.071450798426955	24.22172220445291
37	24.29004202605659	26.01283787193896	25.473338859193667	24.223781242810784
38	23.8123525000449	26.26087497700998	25.899614263040526	24.027158259904596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	136.0
2	199.0
3	199.0
4	616.5
5	1034.0
6	1034.0
7	1277.5
8	1521.0
9	1586.0
10	1651.0
11	1651.0
12	2565.0
13	3479.0
14	5789.0
15	8099.0
16	8099.0
17	13647.5
18	19196.0
19	19196.0
20	27027.5
21	34859.0
22	47182.5
23	59506.0
24	59506.0
25	79560.5
26	99615.0
27	99615.0
28	131087.5
29	162560.0
30	200261.5
31	237963.0
32	237963.0
33	291211.0
34	344459.0
35	344459.0
36	371303.0
37	398147.0
38	428922.0
39	459697.0
40	459697.0
41	466004.0
42	472311.0
43	493816.5
44	515322.0
45	515322.0
46	512497.0
47	509672.0
48	509672.0
49	529942.0
50	550212.0
51	546252.5
52	542293.0
53	542293.0
54	516300.5
55	490308.0
56	490308.0
57	464969.0
58	439630.0
59	392049.5
60	344469.0
61	344469.0
62	316473.5
63	288478.0
64	237251.0
65	186024.0
66	186024.0
67	153395.5
68	120767.0
69	120767.0
70	95861.0
71	70955.0
72	54786.5
73	38618.0
74	38618.0
75	28706.0
76	18794.0
77	18794.0
78	17812.0
79	16830.0
80	13263.0
81	9696.0
82	9696.0
83	9057.0
84	8418.0
85	8418.0
86	5397.0
87	2376.0
88	1908.0
89	1440.0
90	1440.0
91	885.0
92	330.0
93	248.5
94	167.0
95	167.0
96	133.0
97	99.0
98	99.0
99	79.0
100	59.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008561264751152054
2	1.548149141257153E-5
3	0.0
4	2.477038626011445E-4
5	4.644447423771459E-5
6	1.5481491412571528E-4
7	4.4896325096457435E-4
8	0.002461557134598873
9	0.0037000764476045954
10	9.753339589920062E-4
11	0.005077929183323461
12	5.108892166148604E-4
13	1.8577789695085835E-4
14	3.096298282514306E-5
15	0.0
16	3.096298282514306E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5481491412571528E-4
22	3.096298282514306E-5
23	2.63185354013716E-4
24	0.0
25	4.644447423771459E-5
26	6.192596565028612E-5
27	9.288894847542917E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.096298282514306E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	6459326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.28871019493325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60073141776488	43.19085706993025
2	10.09848002417167	10.560729907864701
3	2.7335648272654343	4.288037371558352
4	1.2734554386277013	2.663493695062619
5	0.7215093302479791	1.8863396136138484
6	0.47919888968405094	1.503401512105388
7	0.3269551190415404	1.1967243026419105
8	0.2419982761027654	1.0123022181448753
9	0.18890455360346836	0.8889817912087492
>10	1.1564889423759712	11.48623926941571
>50	0.09407034970234632	3.4069463455894544
>100	0.06721941044394274	7.186363142788492
>500	0.010096681816765353	3.7262862433165282
>1k	0.007296955659086809	6.892072264906074
>5k	2.9783492478256403E-5	0.11122525185283634
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7142	0.11056881166858586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.192596565028611E-5
2	0.0	0.0	0.0	0.0	6.192596565028611E-5
3	0.0	0.0	0.0	0.0	6.192596565028611E-5
4	0.0	0.0	0.0	0.0	7.740745706285764E-5
5	0.0	0.0	0.0	0.0	9.288894847542917E-5
6	0.0	0.0	0.0	0.0	1.3933342271314376E-4
7	0.0	0.0	0.0	0.0	1.5481491412571528E-4
8	0.0	0.0	0.0	0.0	1.5481491412571528E-4
9	0.0	0.0	0.0	0.0	1.5481491412571528E-4
10	0.0	1.5481491412571527E-5	0.0	1.5481491412571527E-5	1.702964055382868E-4
11	0.0	1.5481491412571527E-5	0.0	3.0962982825143053E-5	1.702964055382868E-4
12	0.0	3.0962982825143053E-5	0.0	4.644447423771459E-5	3.0962982825143056E-4
13	0.0	4.644447423771459E-5	0.0	4.644447423771459E-5	3.560743024891452E-4
14	0.0	4.644447423771459E-5	0.0	7.740745706285764E-5	3.560743024891452E-4
15	0.0	7.740745706285764E-5	0.0	1.083704398880007E-4	4.1800026813943125E-4
16	0.0	7.740745706285764E-5	0.0	1.3933342271314376E-4	4.4896325096457435E-4
17	0.0	7.740745706285764E-5	0.0	1.5481491412571528E-4	4.4896325096457435E-4
18	0.0	7.740745706285764E-5	0.0	2.63185354013716E-4	4.799262337897174E-4
19	0.0	7.740745706285764E-5	0.0	2.9414833683885904E-4	4.799262337897174E-4
20	0.0	7.740745706285764E-5	0.0	3.405928110765736E-4	6.037781650902897E-4
21	0.0	7.740745706285764E-5	0.0	4.1800026813943125E-4	6.347411479154327E-4
22	0.0	7.740745706285764E-5	0.0	5.108892166148604E-4	6.657041307405757E-4
23	0.0	7.740745706285764E-5	0.0	7.740745706285764E-4	6.966671135657188E-4
24	0.0	7.740745706285764E-5	0.0	0.00111466738170515	7.121486049782903E-4
25	0.0	7.740745706285764E-5	0.0	0.0014552601927817236	7.276300963908618E-4
26	0.0	7.740745706285764E-5	0.0	0.001733927038208011	7.740745706285764E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6135	0.0	21.881731	1
GGTATCA	2060	0.0	18.874407	1
ACCGTCG	1015	0.0	14.346521	8
CTAGCGG	1020	0.0	14.273986	29
TCAACGC	9265	0.0	14.127512	4
TCTAGCG	1050	0.0	14.018532	28
TATCAAC	9530	0.0	13.918073	2
TACCGTC	1065	0.0	13.823228	7
CAACGCA	9545	0.0	13.679874	5
ATACCGT	1100	0.0	13.674341	6
CGTCGTA	1070	0.0	13.60782	10
TAGGACC	3340	0.0	13.558068	4
TAGCGGC	1105	0.0	13.465568	30
AACGCAG	9770	0.0	13.397692	6
ATCAACG	9825	0.0	13.32197	3
AACGAAT	1050	0.0	12.951904	31
CCGTCGT	1080	0.0	12.741949	9
GTAGGAC	5020	0.0	12.526697	3
TGTAGGA	5075	0.0	12.327022	2
GTCGTAG	1140	0.0	12.21055	11
>>END_MODULE