FastQCFastQC Report
Thu 2 Feb 2017
SRR4062728_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062728_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608203
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31930.1985445867219499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22180.13791791210437987No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16730.1040291555232766No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16620.10334516227118093No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16460.10235026299540544No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16230.10092009528647815No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16170.10054700805806231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT659.384079E-619.7052921
TATACTG2500.017.9167615
ATAATAC2550.016.3128053
TCGAACT805.6258075E-515.99909819
ACCGTCG2900.015.9981048
TAATACC1950.015.5869264
ATCGTTT2300.015.30396129
TACCGTC3050.015.2113117
CTATACT1704.7293724E-1115.0561024
TCTAGCG3750.014.93249228
CTAGCGG3650.014.90373429
CGCTTCG3900.014.76885832
CATCGTT2400.014.6658428
AAACGCT4100.014.43865929
TACGACG5000.014.3973985
CGAACCC1901.8189894E-1114.316318510
ATACCGT3800.014.3132026
GAACTAC5500.014.2639441
GTATAAT3150.014.23161
TAGCGGC4050.014.22186330
Produced by FastQC (version 0.11.5)