##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062728_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1608203
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264932971770357	32.0	32.0	32.0	32.0	32.0
2	30.86870936069638	32.0	32.0	32.0	32.0	32.0
3	30.924763851329715	32.0	32.0	32.0	32.0	32.0
4	30.99583323747064	32.0	32.0	32.0	32.0	32.0
5	30.893270936567088	32.0	32.0	32.0	32.0	32.0
6	34.569528224981546	36.0	36.0	36.0	32.0	36.0
7	34.51251552198323	36.0	36.0	36.0	32.0	36.0
8	34.48048971429602	36.0	36.0	36.0	32.0	36.0
9	34.63674299824089	36.0	36.0	36.0	32.0	36.0
10	34.34445589269514	36.0	36.0	36.0	32.0	36.0
11	34.62488131162546	36.0	36.0	36.0	32.0	36.0
12	34.442142565335345	36.0	36.0	36.0	32.0	36.0
13	34.52564383973914	36.0	36.0	36.0	32.0	36.0
14	34.42785208086293	36.0	36.0	36.0	32.0	36.0
15	34.365330123125005	36.0	36.0	36.0	32.0	36.0
16	34.381584911855036	36.0	36.0	36.0	32.0	36.0
17	34.320018057421855	36.0	36.0	36.0	32.0	36.0
18	34.338665578910124	36.0	36.0	36.0	32.0	36.0
19	34.34005719427211	36.0	36.0	36.0	32.0	36.0
20	34.315966330121256	36.0	36.0	36.0	32.0	36.0
21	34.29717765729824	36.0	36.0	36.0	32.0	36.0
22	34.26891940880598	36.0	36.0	36.0	32.0	36.0
23	34.20910855159455	36.0	36.0	36.0	32.0	36.0
24	34.20105359833305	36.0	36.0	36.0	32.0	36.0
25	34.1742976477472	36.0	36.0	36.0	32.0	36.0
26	34.13169543894645	36.0	36.0	36.0	32.0	36.0
27	34.13288496539305	36.0	36.0	36.0	32.0	36.0
28	34.100371035248656	36.0	36.0	36.0	32.0	36.0
29	34.064982468009326	36.0	36.0	36.0	32.0	36.0
30	34.05102651841838	36.0	36.0	36.0	32.0	36.0
31	34.06653078000725	36.0	36.0	36.0	32.0	36.0
32	34.022301289078555	36.0	36.0	36.0	32.0	36.0
33	33.9785344263131	36.0	36.0	36.0	32.0	36.0
34	33.99522261804014	36.0	36.0	36.0	32.0	36.0
35	33.95048510666875	36.0	36.0	36.0	32.0	36.0
36	33.89839964233371	36.0	36.0	36.0	32.0	36.0
37	33.89043112094679	36.0	36.0	36.0	32.0	36.0
38	33.303421893877825	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	3.0
9	8.0
10	6.0
11	20.0
12	4.0
13	8.0
14	171.0
15	364.0
16	488.0
17	593.0
18	708.0
19	922.0
20	1248.0
21	1921.0
22	2886.0
23	4453.0
24	6595.0
25	10085.0
26	15074.0
27	21799.0
28	30502.0
29	41778.0
30	55636.0
31	75713.0
32	102718.0
33	153922.0
34	342785.0
35	737789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07672184463632	17.481324595005656	11.621241183696009	25.820712376662016
2	16.296672353143926	19.860737300926633	38.39859094817342	25.443999397756016
3	18.351111959637244	24.582202800094773	28.93029115985714	28.13639408041084
4	12.277027506003764	15.822947745184242	36.87703571415247	35.02298903465953
5	14.200392736733322	36.95611620928217	34.18519564084611	14.6582954131384
6	33.47738708465883	36.27300403682138	17.275444037915776	12.974164840604004
7	29.645778328841775	30.76752795979609	21.679987961711277	17.906705749650854
8	27.61794730371963	33.65302882669225	19.816912977701115	18.912110891887004
9	27.620621105498895	14.13551946683149	18.720467703894485	39.523391723775134
10	15.362636597325944	27.053514687904567	32.474875543622225	25.108973171147262
11	36.95068876296269	21.20350235950983	22.78554608241775	19.060262795109733
12	24.43059289972913	23.89750050367997	29.555194619454234	22.11671197713666
13	29.585539015701805	19.79355310553444	25.20696929292125	25.413938585842505
14	23.410341718230153	19.987127265943222	25.69615372656323	30.906377289263393
15	24.915336145821477	27.461253653218098	22.675670111684763	24.947740089275662
16	25.336063799884084	25.884188583780883	24.320845159412645	24.45890245692239
17	23.844814289623603	26.05070030101749	25.688048859367612	24.416436549991293
18	24.64351296511523	24.973960325174133	26.69969896581294	23.6828277438977
19	25.28735918094238	25.32025530877587	25.756237827188993	23.63614768309276
20	25.569346011515332	24.49025786059099	25.546151181785902	24.394244946107776
21	26.60760291848792	24.634582701373613	24.64963158225302	24.108182797885448
22	25.504606096937604	24.711427125166193	25.19479679569376	24.589169982202446
23	24.037270402579054	24.533529183498835	25.719077729796425	25.710122684125693
24	24.48627900699887	25.45812307910451	25.49015096015234	24.565446953744278
25	24.63843286807705	24.84086692774752	25.493071531054508	25.027628673120923
26	24.39593982502763	25.516897390465022	25.9931053397856	24.094057444721752
27	25.146589770648966	25.231163781925915	24.935900983732562	24.686345463692565
28	24.28280670029208	25.026848304437078	25.83798735035996	24.852357644910885
29	24.34111843823615	25.319459611399804	25.73723840629641	24.60218354406764
30	24.38972217333961	25.314194287760987	26.061880620161787	24.234202918737612
31	24.813258346475102	25.213792142796738	24.964243606134925	25.00870590459324
32	24.35930601330763	25.370312791493067	24.967477146943597	25.302904048255705
33	24.11458747910531	24.95796187216429	25.53810047361299	25.389350175117407
34	24.774455144450076	25.1372050286226	25.735838730841827	24.352501096085497
35	25.386318957319737	24.971314103261765	25.65523563481812	23.98713130460038
36	24.421942670643517	25.579301132109265	25.348824482018344	24.649931715228874
37	25.285113289740025	25.432061913690223	25.066524879589814	24.216299916979935
38	24.464260729243424	25.390444031950647	25.465066024917526	24.680229213888396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	281.0
1	248.0
2	215.0
3	215.0
4	560.5
5	906.0
6	906.0
7	1017.5
8	1129.0
9	1080.0
10	1031.0
11	1031.0
12	1443.5
13	1856.0
14	2551.0
15	3246.0
16	3246.0
17	4941.0
18	6636.0
19	6636.0
20	8347.5
21	10059.0
22	11619.0
23	13179.0
24	13179.0
25	16245.5
26	19312.0
27	19312.0
28	24071.0
29	28830.0
30	35398.5
31	41967.0
32	41967.0
33	51977.5
34	61988.0
35	61988.0
36	67892.5
37	73797.0
38	83355.5
39	92914.0
40	92914.0
41	99817.0
42	106720.0
43	117749.5
44	128779.0
45	128779.0
46	133793.0
47	138807.0
48	138807.0
49	144972.5
50	151138.0
51	150051.0
52	148964.0
53	148964.0
54	141707.0
55	134450.0
56	134450.0
57	128934.0
58	123418.0
59	109850.5
60	96283.0
61	96283.0
62	90012.0
63	83741.0
64	68669.0
65	53597.0
66	53597.0
67	43879.0
68	34161.0
69	34161.0
70	27159.0
71	20157.0
72	15617.5
73	11078.0
74	11078.0
75	8268.0
76	5458.0
77	5458.0
78	5229.0
79	5000.0
80	3873.5
81	2747.0
82	2747.0
83	2882.5
84	3018.0
85	3018.0
86	2002.0
87	986.0
88	874.5
89	763.0
90	763.0
91	546.5
92	330.0
93	319.0
94	308.0
95	308.0
96	259.0
97	210.0
98	210.0
99	477.0
100	744.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12100462441619621
2	0.05478164137238894
3	0.011565704080890285
4	0.0027981542131186176
5	6.218120473596927E-5
6	4.3526843315178494E-4
7	6.218120473596927E-5
8	3.730872284158157E-4
9	3.730872284158157E-4
10	5.596308426237235E-4
11	0.0018654361420790784
12	0.002425066984702802
13	0.01461258311295278
14	0.0095137243246033
15	0.023255770571252513
16	0.010881710828794624
17	0.020084529129718077
18	0.004974496378877542
19	0.006902113725692589
20	0.005347583607293358
21	0.006839932520956621
22	0.007275200954108405
23	0.010632986009850746
24	0.014239495884536964
25	0.016913287688183645
26	0.018156911782903032
27	0.008954093481979576
28	0.005845033245181112
29	0.010881710828794624
30	0.0026116105989107095
31	0.006155939268860959
32	0.006404664087804836
33	0.00864318745829973
34	0.01293369058508161
35	0.016788925278711706
36	0.014363858294008902
37	0.009824630348283146
38	0.0060937580641249895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1608203.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.55487629193362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59269971628515	45.01355286195527
2	12.373003239099036	13.995073350938798
3	3.6195177959794114	6.141041435642035
4	1.4952275275717508	3.3824963140045656
5	0.7630367869010605	2.1576725544691993
6	0.46927731387582017	1.5923952259714749
7	0.3133184214596565	1.2403779195944356
8	0.22593256402891923	1.022207056717992
9	0.1674153523183918	0.8521339085763416
>10	0.8386032759147909	8.428205471365716
>50	0.0647879371052069	2.5436998055546334
>100	0.06612299395194494	7.933623390302492
>500	0.0075188113459503615	2.874524011800441
>1k	0.00353826416280017	2.8229966931064894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3193	0.1985445867219499	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2218	0.13791791210437987	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1673	0.1040291555232766	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1662	0.10334516227118093	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1646	0.10235026299540544	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1623	0.10092009528647815	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1617	0.10054700805806231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	6.218120473596927E-5	0.0	0.0	0.0	0.0
4	6.218120473596927E-5	0.0	0.0	0.0	0.0
5	6.218120473596927E-5	0.0	0.0	0.0	0.0
6	6.218120473596927E-5	0.0	0.0	0.0	1.2436240947193855E-4
7	6.218120473596927E-5	0.0	0.0	0.0	1.8654361420790782E-4
8	6.218120473596927E-5	0.0	0.0	0.0	1.8654361420790782E-4
9	6.218120473596927E-5	0.0	0.0	0.0	1.8654361420790782E-4
10	6.218120473596927E-5	0.0	0.0	0.0	1.8654361420790782E-4
11	6.218120473596927E-5	0.0	0.0	6.218120473596927E-5	1.8654361420790782E-4
12	6.218120473596927E-5	0.0	0.0	6.218120473596927E-5	2.487248189438771E-4
13	6.218120473596927E-5	0.0	0.0	6.218120473596927E-5	2.487248189438771E-4
14	6.218120473596927E-5	0.0	0.0	6.218120473596927E-5	3.109060236798464E-4
15	1.2436240947193855E-4	0.0	0.0	1.2436240947193855E-4	4.3526843315178494E-4
16	1.2436240947193855E-4	0.0	0.0	1.2436240947193855E-4	4.3526843315178494E-4
17	1.2436240947193855E-4	0.0	0.0	1.2436240947193855E-4	4.3526843315178494E-4
18	1.2436240947193855E-4	0.0	0.0	1.2436240947193855E-4	4.3526843315178494E-4
19	1.2436240947193855E-4	0.0	0.0	1.8654361420790782E-4	4.3526843315178494E-4
20	1.2436240947193855E-4	0.0	0.0	2.487248189438771E-4	4.3526843315178494E-4
21	1.2436240947193855E-4	0.0	0.0	3.7308722841581564E-4	4.974496378877542E-4
22	1.2436240947193855E-4	0.0	0.0	4.974496378877542E-4	5.596308426237235E-4
23	1.2436240947193855E-4	0.0	0.0	8.705368663035699E-4	6.218120473596928E-4
24	1.2436240947193855E-4	0.0	0.0	0.0014301677089272934	7.461744568316313E-4
25	1.2436240947193855E-4	0.0	0.0	0.0019897985515510168	7.461744568316313E-4
26	1.2436240947193855E-4	0.0	0.0	0.0026116105989107095	7.461744568316313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAT	65	9.384079E-6	19.705292	1
TATACTG	250	0.0	17.916761	5
ATAATAC	255	0.0	16.312805	3
TCGAACT	80	5.6258075E-5	15.999098	19
ACCGTCG	290	0.0	15.998104	8
TAATACC	195	0.0	15.586926	4
ATCGTTT	230	0.0	15.303961	29
TACCGTC	305	0.0	15.211311	7
CTATACT	170	4.7293724E-11	15.056102	4
TCTAGCG	375	0.0	14.932492	28
CTAGCGG	365	0.0	14.903734	29
CGCTTCG	390	0.0	14.768858	32
CATCGTT	240	0.0	14.66584	28
AAACGCT	410	0.0	14.438659	29
TACGACG	500	0.0	14.397398	5
CGAACCC	190	1.8189894E-11	14.3163185	10
ATACCGT	380	0.0	14.313202	6
GAACTAC	550	0.0	14.263944	1
GTATAAT	315	0.0	14.2316	1
TAGCGGC	405	0.0	14.221863	30
>>END_MODULE