FastQCFastQC Report
Thu 2 Feb 2017
SRR4062728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608203
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35490.22068109560795499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26380.16403401809348697No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17610.10950110154004189No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17070.10614331648429956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16990.10564586684641179No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16940.10533496082273194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1850.017.297979
ATAGCGT3600.016.890076
CGCGTAA1900.016.84171110
GCGTAAC1900.016.84171111
ATCGTTT3150.016.76151529
TAGCGTA3550.016.6772237
AACGCTT3200.016.49961530
ACCGTCG3150.016.2551068
AGGACGT3300.016.001125
TAGGACG3200.016.001124
AGCGTAT3750.015.7877718
CTAGCGG3350.015.76082829
TCGTTTA3400.015.52904930
CATCGTT3400.015.52904928
CGTATAT3850.015.37626610
CGTTTAT3450.015.3039931
AAACGCT3350.015.28322729
AACCGCG3050.015.2141797
AATAGCG3900.015.1805495
TAAACGC3400.015.05847228
Produced by FastQC (version 0.11.5)