##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1608203
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173608058186684	32.0	32.0	32.0	32.0	32.0
2	31.256277969883154	32.0	32.0	32.0	32.0	32.0
3	31.366182627441933	32.0	32.0	32.0	32.0	32.0
4	31.465533890932925	32.0	32.0	32.0	32.0	32.0
5	31.386224873352432	32.0	32.0	32.0	32.0	32.0
6	34.916579561162365	36.0	36.0	36.0	36.0	36.0
7	34.94388643722217	36.0	36.0	36.0	36.0	36.0
8	34.87595222742402	36.0	36.0	36.0	32.0	36.0
9	34.99321043425488	36.0	36.0	36.0	36.0	36.0
10	34.82728175485309	36.0	36.0	36.0	32.0	36.0
11	35.017946739310894	36.0	36.0	36.0	36.0	36.0
12	34.915255723313535	36.0	36.0	36.0	32.0	36.0
13	34.974822830202406	36.0	36.0	36.0	36.0	36.0
14	34.91589432428618	36.0	36.0	36.0	32.0	36.0
15	34.8832348901227	36.0	36.0	36.0	32.0	36.0
16	34.903871588350476	36.0	36.0	36.0	32.0	36.0
17	34.87056236059751	36.0	36.0	36.0	32.0	36.0
18	34.86804215636956	36.0	36.0	36.0	32.0	36.0
19	34.862290394931485	36.0	36.0	36.0	32.0	36.0
20	34.852168538424564	36.0	36.0	36.0	32.0	36.0
21	34.84048966455106	36.0	36.0	36.0	32.0	36.0
22	34.82592807002599	36.0	36.0	36.0	32.0	36.0
23	34.76353109650958	36.0	36.0	36.0	32.0	36.0
24	34.74106316180234	36.0	36.0	36.0	32.0	36.0
25	34.729428436584186	36.0	36.0	36.0	32.0	36.0
26	34.65553291468801	36.0	36.0	36.0	32.0	36.0
27	34.638558067607136	36.0	36.0	36.0	32.0	36.0
28	34.61363521893691	36.0	36.0	36.0	32.0	36.0
29	34.577261079602515	36.0	36.0	36.0	32.0	36.0
30	34.556677857210815	36.0	36.0	36.0	32.0	36.0
31	34.549303788141174	36.0	36.0	36.0	32.0	36.0
32	34.51981435179514	36.0	36.0	36.0	32.0	36.0
33	34.48382822317829	36.0	36.0	36.0	32.0	36.0
34	34.478159162742514	36.0	36.0	36.0	32.0	36.0
35	34.443156118972546	36.0	36.0	36.0	32.0	36.0
36	34.413151200439245	36.0	36.0	36.0	32.0	36.0
37	34.40248339295474	36.0	36.0	36.0	32.0	36.0
38	33.9735058322861	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	42.0
22	118.0
23	384.0
24	1067.0
25	2591.0
26	5528.0
27	10613.0
28	18605.0
29	29674.0
30	44533.0
31	64701.0
32	93083.0
33	148573.0
34	363480.0
35	825200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41380253872387	18.692616905423456	12.305696581951329	26.587883973901345
2	15.326050256093291	20.833315197148618	37.61347292599255	26.22716162076554
3	17.31783860619586	25.36278069373083	29.292943739067766	28.026436961005547
4	11.785653658964272	16.767866703229263	36.76860044235764	34.67787919544883
5	13.69517013410007	37.53707556637785	33.880321004450934	14.887433295071142
6	33.49448668976912	36.707397529783314	16.91817990186538	12.879935878582192
7	29.34351866533598	31.06700369047286	21.660619514424557	17.9288581297666
8	27.50764225539186	33.91327003962282	19.639477068259207	18.939610636726105
9	27.427750607065903	13.979149890090756	18.54950890622425	40.04359059661909
10	15.088777265259779	27.383219063513952	32.47810260281486	25.049901068411412
11	37.10482508424449	21.04268532700046	22.738457462983103	19.114032125771942
12	24.230287564093477	24.079684217988085	29.45271664309992	22.237311574818523
13	29.57523939808481	19.759047382166397	25.268125854993162	25.397587364755626
14	23.31627288346061	19.947108667251584	25.701481715927656	31.03513673336015
15	24.885539947382266	27.550253295137495	22.638062483405392	24.92614427407485
16	25.230272546438474	25.97688227170326	24.245819713058616	24.547025468799646
17	23.811919266411017	26.21348175572362	25.5751916891089	24.399407288756457
18	24.69520327968546	25.025012389605045	26.607586231340196	23.672198099369297
19	25.277281537218872	25.464322601064666	25.590674809088153	23.667721052628306
20	25.52140494701229	24.49410926356934	25.52637944339116	24.45810634602721
21	26.4910915986248	24.716499989740086	24.664578619214886	24.127829792420226
22	25.420562839742765	24.769338677603933	25.188688983100384	24.621409499552918
23	23.96474319114538	24.46766571321975	25.76122372839199	25.80636736724288
24	24.44069560870114	25.45580377601584	25.57705712525098	24.526443490032044
25	24.649313550590318	24.725236801572937	25.589493366198173	25.035956281638573
26	24.45599218506619	25.446041326872294	25.94579166933528	24.152174818726245
27	25.11564163932245	25.094251278291708	25.012296348528572	24.777810733857272
28	24.19265478300936	24.879508370522878	25.964446030756065	24.963390815711698
29	24.392194269007085	25.10155745263502	25.859857244390167	24.646391033967728
30	24.416196214035168	25.180527582649702	26.11722525079234	24.286050952522782
31	24.782008241496875	25.062507656060834	25.0641865485887	25.091297553853586
32	24.493363089112506	25.2370502977547	24.98453242532193	25.285054187810868
33	24.101372774456955	24.833680822632466	25.669334033079156	25.395612369831422
34	24.794506663648804	24.94653970922825	25.837409829480485	24.42154379764246
35	25.451699816503265	24.860978371511557	25.68382225378264	24.00349955820254
36	24.48994312285203	25.464633507088347	25.421479751001584	24.623943619058043
37	25.31340881717047	25.227101304996946	25.119900908032132	24.339588969800456
38	24.48994312285203	25.171076039529833	25.57991746066883	24.759063376949303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	156.0
1	154.0
2	152.0
3	152.0
4	497.5
5	843.0
6	843.0
7	964.5
8	1086.0
9	1100.5
10	1115.0
11	1115.0
12	1517.0
13	1919.0
14	2602.5
15	3286.0
16	3286.0
17	5192.0
18	7098.0
19	7098.0
20	8738.5
21	10379.0
22	11856.0
23	13333.0
24	13333.0
25	16287.0
26	19241.0
27	19241.0
28	24227.5
29	29214.0
30	35369.0
31	41524.0
32	41524.0
33	52024.0
34	62524.0
35	62524.0
36	68481.5
37	74439.0
38	83804.5
39	93170.0
40	93170.0
41	99585.5
42	106001.0
43	117255.5
44	128510.0
45	128510.0
46	133633.0
47	138756.0
48	138756.0
49	145798.0
50	152840.0
51	150793.0
52	148746.0
53	148746.0
54	141820.0
55	134894.0
56	134894.0
57	129136.0
58	123378.0
59	110042.5
60	96707.0
61	96707.0
62	90232.0
63	83757.0
64	68341.5
65	52926.0
66	52926.0
67	43568.0
68	34210.0
69	34210.0
70	27350.0
71	20490.0
72	15779.0
73	11068.0
74	11068.0
75	8099.0
76	5130.0
77	5130.0
78	5061.5
79	4993.0
80	3820.0
81	2647.0
82	2647.0
83	2547.0
84	2447.0
85	2447.0
86	1537.0
87	627.0
88	528.0
89	429.0
90	429.0
91	254.0
92	79.0
93	66.0
94	53.0
95	53.0
96	36.5
97	20.0
98	20.0
99	18.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009389361915131362
2	0.0
3	0.0
4	1.2436240947193855E-4
5	6.218120473596927E-5
6	2.487248189438771E-4
7	4.974496378877542E-4
8	0.002425066984702802
9	0.0036065098746862184
10	0.0013058052994553548
11	0.005347583607293358
12	3.109060236798464E-4
13	1.8654361420790785E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2436240947193855E-4
22	6.218120473596927E-5
23	1.8654361420790785E-4
24	0.0
25	0.0
26	0.0
27	1.2436240947193855E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1608203.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.76956336050583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0502790822238	42.74779705499018
2	13.384635677820095	14.661413036273089
3	3.862192736178254	6.3459182932380065
4	1.5836769415873773	3.4694917837936776
5	0.8149482843008214	2.2317180846274676
6	0.5240289133734413	1.7220500864246222
7	0.32871340067130966	1.2602442597860242
8	0.23923494315978444	1.0482234701949458
9	0.165404174653704	0.8153202981409391
>10	0.8984107541739453	8.788373785835002
>50	0.06708540200633592	2.5687743299811623
>100	0.06871915027386469	7.9985070352403875
>500	0.008903622405738377	3.3012486149577978
>1k	0.0037669171716585436	3.040919866516689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3549	0.22068109560795499	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2638	0.16403401809348697	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1761	0.10950110154004189	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1707	0.10614331648429956	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1699	0.10564586684641179	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1694	0.10533496082273194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.218120473596927E-5	0.0
12	0.0	0.0	0.0	6.218120473596927E-5	6.218120473596927E-5
13	0.0	0.0	0.0	6.218120473596927E-5	6.218120473596927E-5
14	0.0	0.0	0.0	6.218120473596927E-5	6.218120473596927E-5
15	0.0	0.0	0.0	1.2436240947193855E-4	6.218120473596927E-5
16	0.0	0.0	0.0	1.2436240947193855E-4	6.218120473596927E-5
17	0.0	0.0	0.0	1.2436240947193855E-4	6.218120473596927E-5
18	0.0	0.0	0.0	1.8654361420790782E-4	1.2436240947193855E-4
19	0.0	0.0	0.0	2.487248189438771E-4	1.2436240947193855E-4
20	0.0	0.0	0.0	4.3526843315178494E-4	1.8654361420790782E-4
21	0.0	0.0	0.0	4.974496378877542E-4	1.8654361420790782E-4
22	0.0	0.0	0.0	6.218120473596928E-4	2.487248189438771E-4
23	0.0	0.0	0.0	9.948992757755084E-4	2.487248189438771E-4
24	0.0	0.0	0.0	0.001803254937343109	2.487248189438771E-4
25	0.0	0.0	0.0	0.002487248189438771	2.487248189438771E-4
26	0.0	0.0	0.0	0.003109060236798464	2.487248189438771E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	185	0.0	17.29797	9
ATAGCGT	360	0.0	16.89007	6
CGCGTAA	190	0.0	16.841711	10
GCGTAAC	190	0.0	16.841711	11
ATCGTTT	315	0.0	16.761515	29
TAGCGTA	355	0.0	16.677223	7
AACGCTT	320	0.0	16.499615	30
ACCGTCG	315	0.0	16.255106	8
AGGACGT	330	0.0	16.00112	5
TAGGACG	320	0.0	16.00112	4
AGCGTAT	375	0.0	15.787771	8
CTAGCGG	335	0.0	15.760828	29
TCGTTTA	340	0.0	15.529049	30
CATCGTT	340	0.0	15.529049	28
CGTATAT	385	0.0	15.376266	10
CGTTTAT	345	0.0	15.30399	31
AAACGCT	335	0.0	15.283227	29
AACCGCG	305	0.0	15.214179	7
AATAGCG	390	0.0	15.180549	5
TAAACGC	340	0.0	15.058472	28
>>END_MODULE