FastQCFastQC Report
Thu 2 Feb 2017
SRR4062727_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062727_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556139
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT56080.21939338979609482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT37380.14623617886194765No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC37000.1447495617413607No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA36150.14142423397162673No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG34280.13410851287821202No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC34030.1331304752988785No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33750.13203507321002497No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG33700.13183946569415825No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT33270.1301572410577046No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA31000.12127665983735626No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC29730.11630822893434198No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT29570.11568228488356853No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC28700.11227871410748788No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG28410.11114419051546102No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27900.10914899385362065No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT27640.10813183477111378No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA26450.10347637589348624No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT26300.10288955334588612No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25570.10003368361423223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC1101.0664735E-817.4524773
TCTAGCG7550.016.95262728
TACGCTC957.715753E-716.839124
CTAGCGG7800.016.40991629
GATATAC11650.016.3609331
GTCCTAC20100.016.0969051
GCGCTTA600.002442704316.00029413
TAGCGGC8000.015.99966730
CGGTCCA8050.015.70215210
TGTAGGA21250.015.5886092
CTTGCGC8750.015.5410133
TTAGGAC12400.015.4820353
GTATCAA51150.015.4692181
TAGCGTA6450.015.3783217
TAGGACG22100.015.3456964
CGCCGGT8450.015.3357597
AATAGCG6600.015.2706165
AAGACGG8600.015.2537065
TTCCGAA8000.015.19938720
GTCCTAT11250.015.0917771
Produced by FastQC (version 0.11.5)