##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062727_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2556139
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263480976582258	32.0	32.0	32.0	32.0	32.0
2	30.891933889354217	32.0	32.0	32.0	32.0	32.0
3	30.946537336193376	32.0	32.0	32.0	32.0	32.0
4	31.01045639536817	32.0	32.0	32.0	32.0	32.0
5	30.918144905265326	32.0	32.0	32.0	32.0	32.0
6	34.5770374772264	36.0	36.0	36.0	32.0	36.0
7	34.51608187191698	36.0	36.0	36.0	32.0	36.0
8	34.49514482584867	36.0	36.0	36.0	32.0	36.0
9	34.63324177597541	36.0	36.0	36.0	32.0	36.0
10	34.35291390648161	36.0	36.0	36.0	32.0	36.0
11	34.617978130297296	36.0	36.0	36.0	32.0	36.0
12	34.429751277219275	36.0	36.0	36.0	32.0	36.0
13	34.522604991356104	36.0	36.0	36.0	32.0	36.0
14	34.42442527577725	36.0	36.0	36.0	32.0	36.0
15	34.363792422869025	36.0	36.0	36.0	32.0	36.0
16	34.39029372033367	36.0	36.0	36.0	32.0	36.0
17	34.319704836082856	36.0	36.0	36.0	32.0	36.0
18	34.339824633949874	36.0	36.0	36.0	32.0	36.0
19	34.32677370049125	36.0	36.0	36.0	32.0	36.0
20	34.30210172451498	36.0	36.0	36.0	32.0	36.0
21	34.282005008334835	36.0	36.0	36.0	32.0	36.0
22	34.24530434377786	36.0	36.0	36.0	32.0	36.0
23	34.19981112138268	36.0	36.0	36.0	32.0	36.0
24	34.173948286849814	36.0	36.0	36.0	32.0	36.0
25	34.153808928231214	36.0	36.0	36.0	32.0	36.0
26	34.11500861259892	36.0	36.0	36.0	32.0	36.0
27	34.11346213957848	36.0	36.0	36.0	32.0	36.0
28	34.086851693119975	36.0	36.0	36.0	32.0	36.0
29	34.04457895286602	36.0	36.0	36.0	32.0	36.0
30	34.01399962991058	36.0	36.0	36.0	32.0	36.0
31	34.032518184652716	36.0	36.0	36.0	32.0	36.0
32	33.976450028734746	36.0	36.0	36.0	32.0	36.0
33	33.95029182685292	36.0	36.0	36.0	32.0	36.0
34	33.95385618700705	36.0	36.0	36.0	32.0	36.0
35	33.92261649307804	36.0	36.0	36.0	32.0	36.0
36	33.867249003281906	36.0	36.0	36.0	32.0	36.0
37	33.86722866010025	36.0	36.0	36.0	32.0	36.0
38	33.24316400633925	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	13.0
10	27.0
11	29.0
12	9.0
13	12.0
14	228.0
15	532.0
16	737.0
17	911.0
18	1151.0
19	1490.0
20	2068.0
21	2996.0
22	4565.0
23	7053.0
24	10537.0
25	16054.0
26	23435.0
27	34350.0
28	48495.0
29	65862.0
30	89459.0
31	121120.0
32	165491.0
33	250436.0
34	559604.0
35	1149468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08343249453014	17.413032562213044	11.580709119409404	25.92282582384741
2	16.051495426238553	19.57914349124586	39.43564833117399	24.933712751341595
3	18.993075919953455	23.983132116236277	28.768912887740854	28.25487907606941
4	11.88817672762355	15.460028661198114	36.98014411974012	35.67165049143822
5	14.06281052778182	37.15692303840334	34.03427522522311	14.745991208591732
6	33.80623535873734	36.36827049620286	17.04845446166317	12.777039683396632
7	29.813871624352196	30.53664139547967	21.11712234741538	18.532364632752756
8	27.444463961773508	34.47203255855623	19.458220679440387	18.62528280022988
9	27.536869169170775	13.968756711798644	18.661705005177744	39.832669113852845
10	15.750032862204083	26.719553950349905	31.91671152618335	25.61370166126266
11	37.79365278082063	20.73135426121248	23.120950830959906	18.354042127006977
12	25.047162318969857	23.447399245559385	29.553149253579527	21.952289181891235
13	29.82141131349919	19.27731114137824	25.594020724160043	25.307256820962525
14	23.037144530594343	20.739541473175002	25.095847576628177	31.127466419602477
15	24.611950746985272	28.90086983606981	22.0570298477151	24.430149569229815
16	24.983175811982264	25.56153661960589	25.447992913495504	24.007294654916343
17	23.369910365857717	26.31566384711914	26.04167848706239	24.272747299960752
18	24.36577238581008	24.748560534244554	27.92416850972796	22.961498570217405
19	25.802919022631343	24.499867758196253	25.380918994461492	24.31629422471091
20	25.5975308072021	24.863682263181555	25.307309103329178	24.231477826287172
21	26.767961702150966	24.26366391415222	24.28725577726777	24.681118606429038
22	25.534949959701713	25.13044124666463	25.238972745839106	24.09563604779455
23	23.73616183826737	24.769189537087936	26.05074171960006	25.443906905044635
24	24.882540199266913	25.234958728648298	25.796785936751693	24.085715135333096
25	24.60837401704666	24.966868060340627	25.98217077103609	24.44258715157662
26	23.502725731752935	26.317241368516115	26.880113192604288	23.299919707126655
27	24.53417655749199	25.195116874499195	25.712350698617787	24.558355869391026
28	23.38780573973199	25.81204887532067	25.940453987581247	24.859691397366092
29	23.827208749193517	24.90454240805494	25.85346462444739	25.41478421830415
30	23.429236517282526	25.681813736038027	26.504176365877036	24.38477338080241
31	25.00232786877549	24.910425956949688	25.128189278032036	24.95905689624278
32	24.122733230305755	25.68810031495139	24.997476476456896	25.191689978285957
33	23.582039565602347	25.22384421655405	26.19629743961733	24.99781877822628
34	24.65938497968165	25.419611255574537	26.288843728309292	23.63216003643452
35	25.10153662674588	25.254643686652102	26.331636230459583	23.31218345614244
36	23.467251703618842	25.980187311991536	25.828919731928497	24.72364125246113
37	24.803140364070018	25.646528282604596	25.28113582708435	24.269195526241038
38	24.025178590920667	25.793384223122157	25.55973616392297	24.621701022034202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	317.5
2	273.0
3	273.0
4	771.0
5	1269.0
6	1269.0
7	1481.0
8	1693.0
9	1621.5
10	1550.0
11	1550.0
12	2157.5
13	2765.0
14	3866.5
15	4968.0
16	4968.0
17	7312.0
18	9656.0
19	9656.0
20	12074.5
21	14493.0
22	17274.5
23	20056.0
24	20056.0
25	26479.0
26	32902.0
27	32902.0
28	43892.5
29	54883.0
30	67657.0
31	80431.0
32	80431.0
33	100971.0
34	121511.0
35	121511.0
36	131371.0
37	141231.0
38	154812.5
39	168394.0
40	168394.0
41	167192.0
42	165990.0
43	179125.0
44	192260.0
45	192260.0
46	190106.0
47	187952.0
48	187952.0
49	202676.5
50	217401.0
51	218100.5
52	218800.0
53	218800.0
54	207133.5
55	195467.0
56	195467.0
57	189685.0
58	183903.0
59	165392.0
60	146881.0
61	146881.0
62	142328.0
63	137775.0
64	115535.5
65	93296.0
66	93296.0
67	78227.0
68	63158.0
69	63158.0
70	50644.5
71	38131.0
72	29666.0
73	21201.0
74	21201.0
75	15752.5
76	10304.0
77	10304.0
78	10124.0
79	9944.0
80	7746.0
81	5548.0
82	5548.0
83	5640.0
84	5732.0
85	5732.0
86	3778.0
87	1824.0
88	1622.0
89	1420.0
90	1420.0
91	1026.5
92	633.0
93	574.5
94	516.0
95	516.0
96	448.0
97	380.0
98	380.0
99	783.0
100	1186.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11959443520090261
2	0.05277490778083664
3	0.01079753487584204
4	0.002738505222133851
5	2.7385052221338507E-4
6	2.7385052221338507E-4
7	0.0
8	2.347290190400444E-4
9	2.347290190400444E-4
10	4.3033653490674804E-4
11	0.002308168687227103
12	0.0028558697316538734
13	0.014787928199522796
14	0.010093347818721909
15	0.023472901904004438
16	0.011540843436135515
17	0.020656153675523904
18	0.006102954495041154
19	0.008411123182268257
20	0.006337683514081199
21	0.006768020048987946
22	0.007785179131494805
23	0.009936861806028544
24	0.01451407767730941
25	0.017526433421656645
26	0.019365144070803662
27	0.009193553245735071
28	0.006494169526774561
29	0.011423478926615493
30	0.0034818137824273255
31	0.005985589985521131
32	0.007393964099761398
33	0.00907618873621505
34	0.012558002518642374
35	0.016078937804243038
36	0.015765965778856313
37	0.010054226315548568
38	0.006181197501387836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2556139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.36182076323716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63383970440054	30.34539878140277
2	14.844747396048458	12.577010569338729
3	5.668744511989348	7.204150169084313
4	2.718719694698147	4.60679665649143
5	1.4474206365438804	3.065768678714125
6	0.8645551888223321	2.1974479169290975
7	0.5751894592383792	1.7056250944011668
8	0.39484339241352123	1.3381028015176082
9	0.26752427117720057	1.0199533702881802
>10	1.3591775191364008	10.157868088268964
>50	0.09909634581415863	2.928215199033137
>100	0.09644861405834104	8.79054310691423
>500	0.01800154230600903	5.257347128597965
>1k	0.011598931898201513	8.585332334214575
>5k	9.27914551856121E-5	0.22044010480367443
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5608	0.21939338979609482	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3738	0.14623617886194765	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3700	0.1447495617413607	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3615	0.14142423397162673	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3428	0.13410851287821202	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3403	0.1331304752988785	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3375	0.13203507321002497	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3370	0.13183946569415825	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3327	0.1301572410577046	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3100	0.12127665983735626	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2973	0.11630822893434198	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2957	0.11568228488356853	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2870	0.11227871410748788	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2841	0.11114419051546102	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2790	0.10914899385362065	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2764	0.10813183477111378	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2645	0.10347637589348624	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2630	0.10288955334588612	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2557	0.10003368361423223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5648601269336291E-4
2	0.0	0.0	0.0	0.0	1.5648601269336291E-4
3	0.0	0.0	0.0	0.0	1.5648601269336291E-4
4	0.0	0.0	0.0	3.912150317334073E-5	1.5648601269336291E-4
5	0.0	0.0	0.0	7.824300634668146E-5	2.3472901904004437E-4
6	0.0	0.0	0.0	7.824300634668146E-5	2.738505222133851E-4
7	0.0	0.0	0.0	7.824300634668146E-5	2.738505222133851E-4
8	0.0	0.0	0.0	7.824300634668146E-5	2.738505222133851E-4
9	0.0	0.0	0.0	1.1736450952002219E-4	2.738505222133851E-4
10	0.0	0.0	0.0	1.9560751586670364E-4	2.738505222133851E-4
11	0.0	0.0	0.0	2.738505222133851E-4	2.738505222133851E-4
12	0.0	0.0	0.0	2.738505222133851E-4	6.650655539467924E-4
13	0.0	0.0	0.0	2.738505222133851E-4	8.215515666401554E-4
14	0.0	0.0	0.0	4.3033653490674804E-4	8.215515666401554E-4
15	0.0	0.0	0.0	4.3033653490674804E-4	0.0010171590825068589
16	0.0	0.0	0.0	6.259440507734517E-4	0.001173645095200222
17	0.0	0.0	0.0	7.824300634668146E-4	0.0012127665983735626
18	0.0	0.0	0.0	9.780375793335182E-4	0.001291009604720244
19	0.0	3.912150317334073E-5	0.0	0.0010562805856801998	0.0013692526110669256
20	0.0	3.912150317334073E-5	0.0	0.001291009604720244	0.0015648601269336291
21	0.0	3.912150317334073E-5	0.0	0.0014083741142402663	0.0017604676428003329
22	0.0	3.912150317334073E-5	0.0	0.0020343181650137178	0.0017995891459736736
23	0.0	3.912150317334073E-5	0.0	0.0027385052221338513	0.0018387106491470143
24	0.0	3.912150317334073E-5	0.0	0.0035600567887740064	0.0018387106491470143
25	0.0	3.912150317334073E-5	0.0	0.004146879336374117	0.0018387106491470143
26	0.0	3.912150317334073E-5	0.0	0.005085795412534295	0.0020343181650137178
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAC	110	1.0664735E-8	17.452477	3
TCTAGCG	755	0.0	16.952627	28
TACGCTC	95	7.715753E-7	16.83912	4
CTAGCGG	780	0.0	16.409916	29
GATATAC	1165	0.0	16.360933	1
GTCCTAC	2010	0.0	16.096905	1
GCGCTTA	60	0.0024427043	16.000294	13
TAGCGGC	800	0.0	15.999667	30
CGGTCCA	805	0.0	15.702152	10
TGTAGGA	2125	0.0	15.588609	2
CTTGCGC	875	0.0	15.541013	3
TTAGGAC	1240	0.0	15.482035	3
GTATCAA	5115	0.0	15.469218	1
TAGCGTA	645	0.0	15.378321	7
TAGGACG	2210	0.0	15.345696	4
CGCCGGT	845	0.0	15.335759	7
AATAGCG	660	0.0	15.270616	5
AAGACGG	860	0.0	15.253706	5
TTCCGAA	800	0.0	15.199387	20
GTCCTAT	1125	0.0	15.091777	1
>>END_MODULE