FastQCFastQC Report
Thu 2 Feb 2017
SRR4062727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556139
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT59550.23296855139724404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT42980.16814422063901846No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC38980.15249561936968217No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC37890.14823137552378804No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG36350.14220666403509355No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA35890.14040707488911988No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34340.13434324189725205No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33330.13039197007674466No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32860.12855325942759763No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC32330.12647981975941058No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG31780.12432813708487683No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA30270.1184207901057024No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG29780.11650383645020869No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC29480.11533019135500848No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT29070.11372620972490151No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC28830.11278729364874132No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT27580.10789710575207373No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG27270.10668433915370017No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG26780.10476738549820648No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT26710.10449353497599309No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG26530.10378934791887295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6700.018.1486228
ACCCGTT2500.017.91937430
AATACCG450.008846953517.7799425
CTAGCGG6900.017.390729
TAACCCG2650.016.9050728
TAGCGGC7200.016.66608630
TGTAGGA21700.016.6630152
GTAGGAC22000.016.2912963
ACCGTCG7400.016.0019478
AACGGAC1703.6379788E-1215.99944215
GATATAC11600.015.7238971
CTGTAGG22200.015.7114711
TACCGTC7500.015.5752297
AACGCTT8050.015.50256430
ATACCGT7650.015.4790066
GTATTAG5700.015.438361
TAGGACG23450.015.3521784
TAGCGTA6150.015.351467
GTTTTCG9350.015.05829928
AGCGTAT6300.014.98594958
Produced by FastQC (version 0.11.5)