##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2556139
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176579990368285	32.0	32.0	32.0	32.0	32.0
2	31.24184874140256	32.0	32.0	32.0	32.0	32.0
3	31.351195690062237	32.0	32.0	32.0	32.0	32.0
4	31.458756350887022	32.0	32.0	32.0	32.0	32.0
5	31.36902375027336	32.0	32.0	32.0	32.0	32.0
6	34.89791243746917	36.0	36.0	36.0	36.0	36.0
7	34.929464320993496	36.0	36.0	36.0	36.0	36.0
8	34.851268651665656	36.0	36.0	36.0	32.0	36.0
9	34.98663609451599	36.0	36.0	36.0	36.0	36.0
10	34.81612697901014	36.0	36.0	36.0	32.0	36.0
11	35.0026739547419	36.0	36.0	36.0	36.0	36.0
12	34.89110099255166	36.0	36.0	36.0	32.0	36.0
13	34.954921465538455	36.0	36.0	36.0	36.0	36.0
14	34.89736512763977	36.0	36.0	36.0	32.0	36.0
15	34.861134312335906	36.0	36.0	36.0	32.0	36.0
16	34.88310260122787	36.0	36.0	36.0	32.0	36.0
17	34.84757910270138	36.0	36.0	36.0	32.0	36.0
18	34.8502890492262	36.0	36.0	36.0	32.0	36.0
19	34.84104659410149	36.0	36.0	36.0	32.0	36.0
20	34.82443990721944	36.0	36.0	36.0	32.0	36.0
21	34.814623539643186	36.0	36.0	36.0	32.0	36.0
22	34.79696565797087	36.0	36.0	36.0	32.0	36.0
23	34.740009052715834	36.0	36.0	36.0	32.0	36.0
24	34.71793904791563	36.0	36.0	36.0	32.0	36.0
25	34.69016864888803	36.0	36.0	36.0	32.0	36.0
26	34.62578091410522	36.0	36.0	36.0	32.0	36.0
27	34.60487985981983	36.0	36.0	36.0	32.0	36.0
28	34.564227532227314	36.0	36.0	36.0	32.0	36.0
29	34.531959725194916	36.0	36.0	36.0	32.0	36.0
30	34.49977485574924	36.0	36.0	36.0	32.0	36.0
31	34.503075928187	36.0	36.0	36.0	32.0	36.0
32	34.46654192123355	36.0	36.0	36.0	32.0	36.0
33	34.44209215539531	36.0	36.0	36.0	32.0	36.0
34	34.43473574793859	36.0	36.0	36.0	32.0	36.0
35	34.406961436760675	36.0	36.0	36.0	32.0	36.0
36	34.37038791708902	36.0	36.0	36.0	32.0	36.0
37	34.35015075471248	36.0	36.0	36.0	32.0	36.0
38	33.90182849993682	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	5.0
21	48.0
22	193.0
23	564.0
24	1602.0
25	4017.0
26	8758.0
27	16735.0
28	29464.0
29	46927.0
30	72438.0
31	105576.0
32	153130.0
33	247734.0
34	601781.0
35	1267164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45693533923544	18.682715135504814	12.26980927445417	26.590540250805578
2	15.091315456632055	20.66898552856476	38.49587209459266	25.743826920210523
3	17.968271678496357	24.78894927075562	29.197747070875252	28.04503197987277
4	11.424900709508416	16.439539993177203	37.1056547851914	35.02990451212298
5	13.54456858566768	37.76320458316234	33.639093961635105	15.053132869534872
6	33.816954112361046	36.85243541518739	16.673141285573728	12.657469186877846
7	29.576789250608147	30.78357639646872	21.131783018521	18.50785133440214
8	27.317255806074396	34.75949239321412	19.23454455330501	18.688707247406473
9	27.426420993823232	13.791554709103343	18.465055938354354	40.31696835871907
10	15.573429938461178	27.01194393042553	31.82241765808201	25.592208473031285
11	37.83851350611182	20.657911415680893	23.052329630191846	18.451245448015435
12	24.835200087632476	23.605998129985565	29.459730138920946	22.099071643461013
13	29.765567295534495	19.35998510246748	25.562608758246846	25.311838843751172
14	22.919762970636572	20.774300615107393	25.075318673984476	31.230617740271562
15	24.6467817282237	28.974637138277693	21.921460452659264	24.457120680839346
16	25.002405973386416	25.64982798268325	25.195275059484267	24.152490984446068
17	23.347478364830707	26.49069553729277	25.806225717771998	24.355600380104526
18	24.438655331341526	24.752605394307587	27.75885818415978	23.049881090191104
19	25.74402252772639	24.582583341516248	25.373111556139943	24.30028257461742
20	25.571848792260514	24.896024824941055	25.265488300910082	24.26663808188835
21	26.760224510658077	24.321427216146866	24.22589243064828	24.69245584254678
22	25.470260212868006	25.167342295290783	25.28568488868754	24.076712603153663
23	23.682434613195312	24.679369438625113	26.073857601417767	25.56433834676181
24	24.834291092933523	25.250465643691523	25.666835801965387	24.24840746140957
25	24.608618395649373	25.011570193616382	25.9446578958796	24.43515351485464
26	23.54653778473619	26.302766126085526	26.691203683056237	23.45949240612205
27	24.687575308981994	25.129766178325408	25.538351637002098	24.644306875690493
28	23.43675363507227	25.791985490616902	25.970614274106378	24.80064660020445
29	23.960160226028396	24.7446637291634	25.86091757920833	25.434258465599875
30	23.491054281476867	25.487541952921966	26.515459448801494	24.505944316799674
31	25.012372175378566	24.750844926664787	25.23309569628256	25.003687201674087
32	24.247194694811196	25.597395133832705	25.01651905471494	25.138891116641155
33	23.63791640438959	25.1112322138976	26.217588323639678	25.033263058073135
34	24.63895742758903	25.359614637545143	26.32521157886954	23.676216355996292
35	25.181103218565188	25.17026656218617	26.194897851799137	23.4537323674495
36	23.58298981393422	25.909076149614712	25.766126177019327	24.741807859431745
37	24.871300034935505	25.61981175515103	25.113344775068963	24.395543434844505
38	24.20587167505954	25.545862974427024	25.59672098824163	24.651544362271803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	194.0
1	191.5
2	189.0
3	189.0
4	649.5
5	1110.0
6	1110.0
7	1349.5
8	1589.0
9	1626.5
10	1664.0
11	1664.0
12	2165.5
13	2667.0
14	3874.5
15	5082.0
16	5082.0
17	7738.0
18	10394.0
19	10394.0
20	12517.0
21	14640.0
22	17169.5
23	19699.0
24	19699.0
25	26288.0
26	32877.0
27	32877.0
28	43293.5
29	53710.0
30	66762.0
31	79814.0
32	79814.0
33	101037.0
34	122260.0
35	122260.0
36	131947.5
37	141635.0
38	155268.5
39	168902.0
40	168902.0
41	168141.5
42	167381.0
43	180623.0
44	193865.0
45	193865.0
46	190822.5
47	187780.0
48	187780.0
49	202240.0
50	216700.0
51	217805.0
52	218910.0
53	218910.0
54	207329.5
55	195749.0
56	195749.0
57	189620.0
58	183491.0
59	165329.0
60	147167.0
61	147167.0
62	143282.5
63	139398.0
64	116351.0
65	93304.0
66	93304.0
67	78477.5
68	63651.0
69	63651.0
70	51105.5
71	38560.0
72	29877.0
73	21194.0
74	21194.0
75	15565.0
76	9936.0
77	9936.0
78	9911.0
79	9886.0
80	7586.5
81	5287.0
82	5287.0
83	5117.0
84	4947.0
85	4947.0
86	3096.5
87	1246.0
88	1087.5
89	929.0
90	929.0
91	558.5
92	188.0
93	129.0
94	70.0
95	70.0
96	55.5
97	41.0
98	41.0
99	37.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008411123182268257
2	0.0
3	0.0
4	1.173645095200222E-4
5	0.0
6	1.5648601269336291E-4
7	5.085795412534296E-4
8	0.002582019209440488
9	0.004342486852240821
10	0.0011345235920268812
11	0.004342486852240821
12	3.520935285600666E-4
13	4.3033653490674804E-4
14	0.0
15	0.0
16	3.912150317334073E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	7.824300634668146E-5
22	3.912150317334073E-5
23	1.5648601269336291E-4
24	0.0
25	7.824300634668146E-5
26	3.912150317334073E-5
27	1.173645095200222E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.173645095200222E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2556139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.13661304025574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.23167103015568	27.78724790267642
2	16.08575327390386	12.912553092314122
3	6.122808809117173	7.372464236730152
4	2.9448821188897676	4.72790376220188
5	1.56992031028638	3.1505641999001326
6	0.9682422826431528	2.3317179496597293
7	0.6193705296901618	1.7401604695098576
8	0.42528733301914695	1.3655674493049506
9	0.2982322183481653	1.077302802957963
>10	1.4903365046672485	10.529107601326242
>50	0.10528936745756126	2.9468471093926563
>100	0.10513589766652888	9.021283951153876
>500	0.020313225816469247	5.624967994213466
>1k	0.01265896681316192	9.177763854437474
>5k	9.813152568342574E-5	0.23454762422130213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5955	0.23296855139724404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4298	0.16814422063901846	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3898	0.15249561936968217	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3789	0.14823137552378804	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3635	0.14220666403509355	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3589	0.14040707488911988	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3434	0.13434324189725205	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3333	0.13039197007674466	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3286	0.12855325942759763	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3233	0.12647981975941058	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3178	0.12432813708487683	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3027	0.1184207901057024	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2978	0.11650383645020869	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2948	0.11533019135500848	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2907	0.11372620972490151	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2883	0.11278729364874132	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2758	0.10789710575207373	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2727	0.10668433915370017	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2678	0.10476738549820648	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2671	0.10449353497599309	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2653	0.10378934791887295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5648601269336291E-4
2	0.0	0.0	0.0	0.0	1.5648601269336291E-4
3	0.0	0.0	0.0	0.0	1.5648601269336291E-4
4	0.0	0.0	0.0	0.0	1.5648601269336291E-4
5	0.0	0.0	0.0	0.0	1.9560751586670364E-4
6	0.0	0.0	0.0	0.0	2.738505222133851E-4
7	0.0	0.0	0.0	3.912150317334073E-5	2.738505222133851E-4
8	0.0	0.0	0.0	3.912150317334073E-5	2.738505222133851E-4
9	0.0	0.0	0.0	3.912150317334073E-5	2.738505222133851E-4
10	0.0	0.0	0.0	1.1736450952002219E-4	3.1297202538672583E-4
11	0.0	0.0	0.0	1.1736450952002219E-4	3.1297202538672583E-4
12	0.0	0.0	0.0	1.1736450952002219E-4	8.606730698134961E-4
13	0.0	0.0	0.0	1.5648601269336291E-4	9.780375793335182E-4
14	0.0	0.0	0.0	3.520935285600666E-4	9.780375793335182E-4
15	0.0	0.0	0.0	3.520935285600666E-4	0.0013301311078935847
16	0.0	0.0	0.0	5.085795412534294E-4	0.0014866171205869477
17	0.0	0.0	0.0	5.86822547600111E-4	0.0014866171205869477
18	0.0	0.0	0.0	8.215515666401554E-4	0.0014866171205869477
19	0.0	0.0	0.0	8.997945729868368E-4	0.0014866171205869477
20	0.0	0.0	0.0	0.001173645095200222	0.0016431031332803108
21	0.0	0.0	0.0	0.0013301311078935847	0.0016822246364536515
22	0.0	0.0	0.0	0.0020343181650137178	0.0017213461396269922
23	0.0	0.0	0.0	0.0028167482284805327	0.0017213461396269922
24	0.0	0.0	0.0	0.003638299795120688	0.0017213461396269922
25	0.0	0.0	0.0	0.004381608355414162	0.0017213461396269922
26	0.0	0.0	0.0	0.005085795412534295	0.0017213461396269922
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	670	0.0	18.14862	28
ACCCGTT	250	0.0	17.919374	30
AATACCG	45	0.0088469535	17.779942	5
CTAGCGG	690	0.0	17.3907	29
TAACCCG	265	0.0	16.90507	28
TAGCGGC	720	0.0	16.666086	30
TGTAGGA	2170	0.0	16.663015	2
GTAGGAC	2200	0.0	16.291296	3
ACCGTCG	740	0.0	16.001947	8
AACGGAC	170	3.6379788E-12	15.999442	15
GATATAC	1160	0.0	15.723897	1
CTGTAGG	2220	0.0	15.711471	1
TACCGTC	750	0.0	15.575229	7
AACGCTT	805	0.0	15.502564	30
ATACCGT	765	0.0	15.479006	6
GTATTAG	570	0.0	15.43836	1
TAGGACG	2345	0.0	15.352178	4
TAGCGTA	615	0.0	15.35146	7
GTTTTCG	935	0.0	15.058299	28
AGCGTAT	630	0.0	14.9859495	8
>>END_MODULE