FastQCFastQC Report
Thu 2 Feb 2017
SRR4062726_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062726_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950897
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT80800.16320274891600453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT54430.10993967355814512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64350.019.7616881
GGTATCA25700.017.26231
TAGGACG10450.015.3065884
GTATAGA6250.014.8627821
GACGTAT650.004136716514.7839331
GTATAGG5550.014.4287621
TCTAGCG9450.014.39072628
GTCCTAC12000.014.2808691
CTAGCGG9450.014.22214229
TACCGTC11400.014.1740697
CGCTTCG8700.014.16083932
ACCGTCG11750.014.1604788
CGTCGTA11450.014.11445510
ATACCGT12000.013.9962446
TAAACGC9200.013.91226128
ACGATTA1503.1772288E-813.86658932
GTCCTAT12250.013.8586811
CAAGACG14250.013.6942944
ATAGCGT6800.013.6433976
TAGCGGC10050.013.53226130
Produced by FastQC (version 0.11.5)