##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062726_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4950897
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312961469406453	32.0	32.0	32.0	32.0	32.0
2	30.935821730890382	32.0	32.0	32.0	32.0	32.0
3	30.977931069864713	32.0	32.0	32.0	32.0	32.0
4	31.051591863050273	32.0	32.0	32.0	32.0	32.0
5	30.961442340650592	32.0	32.0	32.0	32.0	32.0
6	34.640063608675355	36.0	36.0	36.0	32.0	36.0
7	34.59246233561312	36.0	36.0	36.0	32.0	36.0
8	34.5702017230413	36.0	36.0	36.0	32.0	36.0
9	34.719478712645405	36.0	36.0	36.0	32.0	36.0
10	34.437054133826656	36.0	36.0	36.0	32.0	36.0
11	34.689735415622664	36.0	36.0	36.0	32.0	36.0
12	34.519840142099504	36.0	36.0	36.0	32.0	36.0
13	34.59465203982228	36.0	36.0	36.0	32.0	36.0
14	34.50360550825436	36.0	36.0	36.0	32.0	36.0
15	34.44563338724276	36.0	36.0	36.0	32.0	36.0
16	34.465521096480096	36.0	36.0	36.0	32.0	36.0
17	34.400765760224864	36.0	36.0	36.0	32.0	36.0
18	34.42695010621308	36.0	36.0	36.0	32.0	36.0
19	34.415400481973265	36.0	36.0	36.0	32.0	36.0
20	34.399925710431866	36.0	36.0	36.0	32.0	36.0
21	34.390904718882254	36.0	36.0	36.0	32.0	36.0
22	34.368706317259274	36.0	36.0	36.0	32.0	36.0
23	34.30825848326071	36.0	36.0	36.0	32.0	36.0
24	34.302852392202865	36.0	36.0	36.0	32.0	36.0
25	34.27663047726503	36.0	36.0	36.0	32.0	36.0
26	34.23384287736142	36.0	36.0	36.0	32.0	36.0
27	34.238028987474394	36.0	36.0	36.0	32.0	36.0
28	34.22208662389866	36.0	36.0	36.0	32.0	36.0
29	34.19520563647355	36.0	36.0	36.0	32.0	36.0
30	34.167341392882946	36.0	36.0	36.0	32.0	36.0
31	34.18596852247179	36.0	36.0	36.0	32.0	36.0
32	34.14244630821445	36.0	36.0	36.0	32.0	36.0
33	34.1178939897154	36.0	36.0	36.0	32.0	36.0
34	34.12150767830557	36.0	36.0	36.0	32.0	36.0
35	34.0953849373154	36.0	36.0	36.0	32.0	36.0
36	34.04735929670926	36.0	36.0	36.0	32.0	36.0
37	34.05361311293691	36.0	36.0	36.0	32.0	36.0
38	33.4931049868337	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	21.0
10	35.0
11	53.0
12	27.0
13	28.0
14	319.0
15	904.0
16	1272.0
17	1540.0
18	1894.0
19	2437.0
20	3235.0
21	4961.0
22	7898.0
23	12430.0
24	18961.0
25	29349.0
26	43333.0
27	62970.0
28	87397.0
29	118646.0
30	161068.0
31	216961.0
32	299053.0
33	456056.0
34	1051103.0
35	2368936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95407834639579	17.3194858878001	11.56052729767525	26.16590846812887
2	16.660233314444202	19.33796965828997	37.80349591728016	26.198301109985668
3	18.702875511658945	23.334900235722184	28.65043646682163	29.31178778579724
4	12.63235118564888	15.582147267009496	35.9746171740882	35.81088437325342
5	14.757026465347941	36.181946313076295	33.89831603980619	15.162711181769579
6	34.34083690912572	35.65858137657841	16.819905293680886	13.180676420614985
7	30.55018110859507	30.38497872203764	20.7829207515325	18.28191941783479
8	28.121058784653407	33.29861899410287	19.145550572382632	19.434771648861094
9	27.50582218550604	14.235642480442912	18.553330815267557	39.705204518783496
10	15.905539198190866	26.63389672823755	31.790415398962484	25.670148674609095
11	37.40339968473859	20.991586834644497	22.560460789058443	19.044552691558476
12	24.75850250546581	23.443334943855266	29.05395033021086	22.744212220468064
13	29.568622285771195	19.702704739217676	25.10947619300617	25.619196782004956
14	23.640777934576345	19.68187891101441	25.16462166408575	31.512721490323493
15	25.256406759663047	27.455751966551205	22.160150555947837	25.127690717837908
16	25.742238048661452	25.670808986648662	23.9698034448362	24.617149519853683
17	24.06289186959792	25.85141830152112	25.21868937290561	24.867000455975347
18	24.680338341118546	25.198434541093295	26.20438833480621	23.916838782981948
19	25.548319746580823	24.914287007075803	25.32181888293644	24.21557436340693
20	25.913108959817915	24.424222934771322	24.911617023666153	24.751051081744606
21	26.78298156338727	24.361428873845995	24.514521923070088	24.34106763969665
22	26.071525602788387	24.3700933333468	24.958090388210564	24.600290675654247
23	24.66723483475015	24.465413289759866	25.39440648689311	25.47294538859687
24	24.901988788733544	25.053114026325	25.180401838390736	24.864495346550715
25	25.008717104797984	24.626699835435605	25.25408895850901	25.1104941012574
26	24.60861528204973	25.544629464082792	25.592569584387643	24.254185669479842
27	25.311541944511383	24.73123188424826	25.047546115439445	24.90968005580091
28	24.687569246419184	24.829026361764605	25.498882260534394	24.984522131281818
29	24.57061500471887	25.016503930238272	25.484956980697877	24.927924084344983
30	24.56707587049402	25.254708779595504	25.573367439936394	24.604847909974083
31	25.176897678302556	24.975184836106802	24.725437431649727	25.122480053940915
32	24.89312848962271	25.046989529086645	24.703392800547654	25.35648918074299
33	24.511512607045045	24.79641392784466	25.299394847293783	25.39267861781651
34	25.169389305621515	24.862356354172075	25.492077927665953	24.476176412540454
35	25.516324362849453	24.657106967434505	25.49434494673426	24.332223722981784
36	24.431348956877144	25.319385164367205	25.176035567507636	25.07323031124801
37	25.404638119946	24.979380499922126	24.851714332405937	24.764267047725934
38	24.61504060023589	25.000207046154326	25.21549464740242	25.169257706207365
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	150.0
2	237.0
3	237.0
4	683.5
5	1130.0
6	1130.0
7	1341.5
8	1553.0
9	1574.5
10	1596.0
11	1596.0
12	2490.5
13	3385.0
14	5229.0
15	7073.0
16	7073.0
17	11274.5
18	15476.0
19	15476.0
20	19942.5
21	24409.0
22	29999.5
23	35590.0
24	35590.0
25	45771.0
26	55952.0
27	55952.0
28	69402.5
29	82853.0
30	102268.5
31	121684.0
32	121684.0
33	152520.5
34	183357.0
35	183357.0
36	204795.0
37	226233.0
38	254616.5
39	283000.0
40	283000.0
41	301527.5
42	320055.0
43	352834.0
44	385613.0
45	385613.0
46	395111.5
47	404610.0
48	404610.0
49	426327.5
50	448045.0
51	452148.0
52	456251.0
53	456251.0
54	444145.5
55	432040.0
56	432040.0
57	413443.0
58	394846.0
59	354035.0
60	313224.0
61	313224.0
62	292463.0
63	271702.0
64	226804.0
65	181906.0
66	181906.0
67	151606.0
68	121306.0
69	121306.0
70	97566.0
71	73826.0
72	57104.5
73	40383.0
74	40383.0
75	30568.0
76	20753.0
77	20753.0
78	18697.0
79	16641.0
80	12762.0
81	8883.0
82	8883.0
83	8450.5
84	8018.0
85	8018.0
86	5481.0
87	2944.0
88	2429.0
89	1914.0
90	1914.0
91	1426.0
92	938.0
93	842.5
94	747.0
95	747.0
96	694.0
97	641.0
98	641.0
99	1330.5
100	2020.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12244245840703209
2	0.05504053103912281
3	0.011674652088298343
4	0.002989357282124835
5	1.6158688011485596E-4
6	2.6257868018664097E-4
7	0.0
8	2.6257868018664097E-4
9	1.4138852010049896E-4
10	3.6357048025842587E-4
11	0.0017168606012203445
12	0.002444001561737196
13	0.014825596250538033
14	0.009190253806532434
15	0.02508636313783139
16	0.010725329167623563
17	0.02157184849533327
18	0.005493953923905103
19	0.007352203045225945
20	0.00571613588406303
21	0.006322086684493739
22	0.007311806325197232
23	0.009776006246948785
24	0.014098455290021182
25	0.017350391252332658
26	0.019632805933955
27	0.008887278406317077
28	0.005655540804019959
29	0.011331279968054274
30	0.002928762202081764
31	0.005958516204235313
32	0.0065846653646803805
33	0.008301525965900725
34	0.012401793048815194
35	0.016380869971643522
36	0.015370951970925672
37	0.0098164029669775
38	0.0062614916044506684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4950897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.56236714850124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.97094400065682	19.52795896791595
2	14.802558908977003	9.640127158628543
3	7.86490618198918	7.682998880593464
4	4.606905728845614	6.000470230448184
5	2.992758244859083	4.872564637790282
6	2.1018520361686854	4.1064766616129775
7	1.489928257657296	3.396091366553454
8	1.083078979865673	2.8214092314568178
9	0.8531798173185855	2.50033990096971
>10	3.9888445095968166	21.44793230976565
>50	0.13696247025094405	3.049163040283512
>100	0.08472744023108893	5.642431168929591
>500	0.01351384922153922	3.1101704381312967
>1k	0.009715022787391177	5.92764025116473
>5k	1.245515741972991E-4	0.27422575575580926
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8080	0.16320274891600453	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5443	0.10993967355814512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8178524012921294E-4
2	0.0	0.0	0.0	2.0198360014356995E-5	1.8178524012921294E-4
3	0.0	0.0	0.0	2.0198360014356995E-5	1.8178524012921294E-4
4	0.0	0.0	0.0	4.039672002871399E-5	1.8178524012921294E-4
5	0.0	0.0	0.0	4.039672002871399E-5	2.4238032017228394E-4
6	0.0	0.0	0.0	4.039672002871399E-5	2.625786801866409E-4
7	0.0	0.0	0.0	6.0595080043070986E-5	2.625786801866409E-4
8	0.0	0.0	0.0	6.0595080043070986E-5	2.625786801866409E-4
9	0.0	0.0	0.0	8.079344005742798E-5	2.625786801866409E-4
10	0.0	0.0	0.0	1.0099180007178497E-4	3.433721202440689E-4
11	0.0	0.0	0.0	1.2119016008614197E-4	3.6357048025842587E-4
12	0.0	0.0	0.0	1.4138852010049896E-4	7.473393205312087E-4
13	0.0	0.0	0.0	1.4138852010049896E-4	9.089262006460648E-4
14	0.0	0.0	0.0	1.6158688011485596E-4	9.089262006460648E-4
15	0.0	0.0	0.0	1.8178524012921294E-4	0.0010907114407752777
16	0.0	0.0	0.0	2.2218196015792694E-4	0.0011311081608039917
17	0.0	0.0	0.0	3.231737602297119E-4	0.0011513065208183487
18	0.0	0.0	0.0	4.039672002871399E-4	0.0012320999608757767
19	2.0198360014356995E-5	0.0	0.0	4.443639203158539E-4	0.0012522983208901337
20	2.0198360014356995E-5	0.0	0.0	5.453557203876388E-4	0.0014542819210337035
21	2.0198360014356995E-5	0.0	0.0	6.059508004307098E-4	0.0015148770010767745
22	2.0198360014356995E-5	0.0	0.0	8.685294806173508E-4	0.0015350753610911315
23	2.0198360014356995E-5	0.0	0.0	0.0010907114407752777	0.0015350753610911315
24	2.0198360014356995E-5	0.0	0.0	0.0016360671611629165	0.0015754720811198455
25	2.0198360014356995E-5	0.0	0.0	0.0019996376414213425	0.0015754720811198455
26	2.0198360014356995E-5	0.0	0.0	0.002403604841708482	0.0016360671611629165
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6435	0.0	19.761688	1
GGTATCA	2570	0.0	17.2623	1
TAGGACG	1045	0.0	15.306588	4
GTATAGA	625	0.0	14.862782	1
GACGTAT	65	0.0041367165	14.783933	1
GTATAGG	555	0.0	14.428762	1
TCTAGCG	945	0.0	14.390726	28
GTCCTAC	1200	0.0	14.280869	1
CTAGCGG	945	0.0	14.222142	29
TACCGTC	1140	0.0	14.174069	7
CGCTTCG	870	0.0	14.160839	32
ACCGTCG	1175	0.0	14.160478	8
CGTCGTA	1145	0.0	14.114455	10
ATACCGT	1200	0.0	13.996244	6
TAAACGC	920	0.0	13.912261	28
ACGATTA	150	3.1772288E-8	13.866589	32
GTCCTAT	1225	0.0	13.858681	1
CAAGACG	1425	0.0	13.694294	4
ATAGCGT	680	0.0	13.643397	6
TAGCGGC	1005	0.0	13.532261	30
>>END_MODULE