FastQCFastQC Report
Thu 2 Feb 2017
SRR4062726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950897
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT84350.17037316672110125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT59620.1204226224055964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57950.020.5990941
GGTATCA21550.018.6375661
CTAGCGG9150.015.03775729
CGTCGTA10900.014.53236810
TCTAGCG9650.014.25859928
TATTCCG1351.3200588E-714.223485
TACCGTC11550.014.1311187
ACCGTCG11350.014.0981628
TAGCGGC10000.013.91954230
ATACCGT12200.013.77170856
TAGGACG9400.013.4473174
TTAGGAC10300.013.3597293
TAAACCG1208.719573E-613.3345125
TATCAAC90150.013.2043592
TCAACGC89600.013.1076394
GTCGTAG12350.012.95569511
AACGCAG92150.012.7976586
CAACGCA92450.012.7388215
ATCAACG92300.012.7242093
GTCCTAC11950.012.7209181
Produced by FastQC (version 0.11.5)