##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4950897
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224053540196856	32.0	32.0	32.0	32.0	32.0
2	31.32515845108472	32.0	32.0	32.0	32.0	32.0
3	31.404026785449183	32.0	32.0	32.0	32.0	32.0
4	31.497825949519854	32.0	32.0	32.0	32.0	32.0
5	31.430440786790758	32.0	32.0	32.0	32.0	32.0
6	34.96290470191563	36.0	36.0	36.0	36.0	36.0
7	34.990018374448105	36.0	36.0	36.0	36.0	36.0
8	34.92204341960659	36.0	36.0	36.0	36.0	36.0
9	35.02773174234891	36.0	36.0	36.0	36.0	36.0
10	34.8835150478792	36.0	36.0	36.0	32.0	36.0
11	35.055046792530725	36.0	36.0	36.0	36.0	36.0
12	34.96049624946752	36.0	36.0	36.0	36.0	36.0
13	35.00684704206127	36.0	36.0	36.0	36.0	36.0
14	34.95532344946784	36.0	36.0	36.0	32.0	36.0
15	34.93037645501411	36.0	36.0	36.0	36.0	36.0
16	34.946043110975644	36.0	36.0	36.0	36.0	36.0
17	34.91677184962644	36.0	36.0	36.0	32.0	36.0
18	34.91363706415221	36.0	36.0	36.0	32.0	36.0
19	34.90999994546443	36.0	36.0	36.0	32.0	36.0
20	34.89900072653501	36.0	36.0	36.0	32.0	36.0
21	34.887671870370156	36.0	36.0	36.0	32.0	36.0
22	34.87553023219833	36.0	36.0	36.0	32.0	36.0
23	34.81888999104607	36.0	36.0	36.0	32.0	36.0
24	34.79958136879034	36.0	36.0	36.0	32.0	36.0
25	34.79020145238327	36.0	36.0	36.0	32.0	36.0
26	34.72260279298882	36.0	36.0	36.0	32.0	36.0
27	34.70436508778106	36.0	36.0	36.0	32.0	36.0
28	34.685111001097376	36.0	36.0	36.0	32.0	36.0
29	34.65513158524607	36.0	36.0	36.0	32.0	36.0
30	34.63263970145208	36.0	36.0	36.0	32.0	36.0
31	34.63346157272107	36.0	36.0	36.0	32.0	36.0
32	34.60568054637372	36.0	36.0	36.0	32.0	36.0
33	34.5818747592608	36.0	36.0	36.0	32.0	36.0
34	34.57589281295895	36.0	36.0	36.0	32.0	36.0
35	34.555607398012924	36.0	36.0	36.0	32.0	36.0
36	34.52783566291119	36.0	36.0	36.0	32.0	36.0
37	34.530197053180466	36.0	36.0	36.0	32.0	36.0
38	34.11839814078136	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	22.0
21	96.0
22	416.0
23	1194.0
24	3189.0
25	8131.0
26	16561.0
27	32023.0
28	55106.0
29	86676.0
30	129342.0
31	185478.0
32	265901.0
33	425183.0
34	1071319.0
35	2670257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33121943947405	18.47711164825948	12.224493184621432	26.96717572764504
2	15.717576027132052	20.261055723841558	37.10879058885693	26.91257766016946
3	17.711113763829058	24.101147731411096	28.9472392578557	29.240499246904143
4	12.160793037852216	16.39225787061144	35.89115160397553	35.55579748756082
5	14.23831161066603	36.728462888333745	33.61716747837159	15.416058022628631
6	34.37851957063056	35.99954190036211	16.53988133037952	13.082057198627803
7	30.330781732048333	30.50933591981059	20.80186585086524	18.358016497275838
8	28.00254909069976	33.56302081387093	19.012439441421915	19.421990654007395
9	27.417373688895673	14.094047035435242	18.331776985565455	40.15680229010363
10	15.60177795875695	26.907724457597755	31.88059172109171	25.60990586255359
11	37.51741189017986	20.954482652747927	22.486850185026583	19.041255272045635
12	24.603893891251317	23.572865688807433	28.975881676056574	22.847358743884676
13	29.5334433876421	19.70274429501449	25.11353011550047	25.650282201842945
14	23.560457832186774	19.69679433848048	25.090402809834256	31.652345019498483
15	25.16245036000547	27.61657130010986	22.031139003699735	25.18983933618494
16	25.724919287337077	25.759902853948052	23.83069650422873	24.684481354486138
17	24.06208410314333	25.96747619673768	25.089776660673813	24.880663039445174
18	24.683688632585167	25.28164088245019	26.099634066311623	23.935036418653024
19	25.522809301021613	25.010053733697145	25.258533958593766	24.208603006687476
20	25.91669751966159	24.497540546692854	24.845659281540293	24.740102652105264
21	26.774049357984246	24.395851171031637	24.497630830490916	24.332468640493197
22	25.99814780963846	24.428169048222596	24.9482366319625	24.625446510176445
23	24.62884451078493	24.418740872853164	25.36533835330617	25.587076263055735
24	24.941682285048547	25.05541925028939	25.077092090584795	24.925806374077265
25	25.001959242900067	24.638509585747375	25.29307849979357	25.066452671558988
26	24.68535487018004	25.451741553799867	25.519769657809345	24.343133918210746
27	25.3653278113699	24.742006295426123	24.961522116400747	24.93114377680323
28	24.702190330358317	24.843255676698586	25.48390725963396	24.970646733309135
29	24.63531356035078	24.907365271384155	25.43803678404136	25.019284384223706
30	24.596653899283304	25.191535998426147	25.545027497037402	24.66678260525315
31	25.13645507066699	24.911122166346825	24.70114001563757	25.251282747348615
32	24.91160692698717	25.04905676688487	24.670660690375907	25.368675615752057
33	24.517637914907137	24.758038795798015	25.31436222567345	25.4099610636214
34	25.14089870987015	24.80790854667346	25.52957575162642	24.521616991829966
35	25.48334170555356	24.6900713143497	25.42242345175026	24.40416352834648
36	24.475746516237358	25.308484502909273	25.174427987493985	25.041340993359384
37	25.401073785215083	24.94762060289277	24.835216729412874	24.816088882479274
38	24.64722504438596	24.89320102042259	25.23629084871609	25.223283086475362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	78.0
2	132.0
3	132.0
4	467.0
5	802.0
6	802.0
7	1072.0
8	1342.0
9	1379.5
10	1417.0
11	1417.0
12	2230.5
13	3044.0
14	4939.5
15	6835.0
16	6835.0
17	11244.0
18	15653.0
19	15653.0
20	20058.5
21	24464.0
22	30134.0
23	35804.0
24	35804.0
25	45802.0
26	55800.0
27	55800.0
28	69894.5
29	83989.0
30	103162.0
31	122335.0
32	122335.0
33	153373.0
34	184411.0
35	184411.0
36	206318.0
37	228225.0
38	256263.0
39	284301.0
40	284301.0
41	303227.5
42	322154.0
43	354946.5
44	387739.0
45	387739.0
46	395781.0
47	403823.0
48	403823.0
49	425990.5
50	448158.0
51	451563.5
52	454969.0
53	454969.0
54	443986.5
55	433004.0
56	433004.0
57	414108.0
58	395212.0
59	354679.5
60	314147.0
61	314147.0
62	292615.0
63	271083.0
64	226432.5
65	181782.0
66	181782.0
67	151730.0
68	121678.0
69	121678.0
70	97691.0
71	73704.0
72	56770.0
73	39836.0
74	39836.0
75	29874.5
76	19913.0
77	19913.0
78	18233.5
79	16554.0
80	12409.0
81	8264.0
82	8264.0
83	7566.5
84	6869.0
85	6869.0
86	4412.5
87	1956.0
88	1505.0
89	1054.0
90	1054.0
91	636.5
92	219.0
93	165.5
94	112.0
95	112.0
96	80.0
97	48.0
98	48.0
99	44.5
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009594221006819573
2	0.0
3	0.0
4	2.221819601579269E-4
5	2.0198360014356995E-5
6	1.8178524012921294E-4
7	3.837688402727829E-4
8	0.0022622163216079834
9	0.004120465442928827
10	0.0010301163607322067
11	0.0051909785236897476
12	2.827770402009979E-4
13	1.6158688011485596E-4
14	0.0
15	0.0
16	2.0198360014356995E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2119016008614197E-4
22	4.039672002871399E-5
23	1.4138852010049896E-4
24	0.0
25	1.0099180007178498E-4
26	4.039672002871399E-5
27	2.0198360014356995E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.4138852010049896E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4950897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.030951290139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.745214521950594	16.740818219670473
2	16.20166421106179	9.731027774831698
3	8.620806076942566	7.766730221351896
4	5.067127900715272	6.086826846691386
5	3.413200391085822	5.1250827344090855
6	2.291289806061941	4.128576753446291
7	1.6703051911512155	3.511259768458993
8	1.2215480748679135	2.9347400587937105
9	0.9589384299061375	2.591804995087823
>10	4.528700679754377	22.503513944302174
>50	0.16128278105511965	3.2959960200782303
>100	0.0933486156933858	5.733208500981336
>500	0.015213734136838327	3.1847579887169055
>1k	0.01122435245502142	6.373312094592612
>5k	1.3523316210867548E-4	0.29234407858743006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8435	0.17037316672110125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5962	0.1204226224055964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.079344005742798E-5
2	0.0	0.0	0.0	2.0198360014356995E-5	8.079344005742798E-5
3	0.0	0.0	0.0	2.0198360014356995E-5	8.079344005742798E-5
4	0.0	0.0	0.0	6.0595080043070986E-5	8.079344005742798E-5
5	0.0	0.0	0.0	6.0595080043070986E-5	8.079344005742798E-5
6	0.0	0.0	0.0	6.0595080043070986E-5	8.079344005742798E-5
7	0.0	0.0	0.0	6.0595080043070986E-5	1.0099180007178497E-4
8	0.0	0.0	0.0	6.0595080043070986E-5	1.0099180007178497E-4
9	0.0	0.0	0.0	6.0595080043070986E-5	1.0099180007178497E-4
10	0.0	0.0	0.0	8.079344005742798E-5	1.0099180007178497E-4
11	0.0	0.0	0.0	8.079344005742798E-5	1.0099180007178497E-4
12	0.0	0.0	0.0	1.0099180007178497E-4	5.251573603732818E-4
13	0.0	0.0	0.0	1.0099180007178497E-4	6.665458804737808E-4
14	0.0	0.0	0.0	1.4138852010049896E-4	6.665458804737808E-4
15	0.0	0.0	0.0	1.6158688011485596E-4	8.685294806173508E-4
16	0.0	0.0	0.0	2.0198360014356994E-4	9.493229206747788E-4
17	0.0	0.0	2.0198360014356995E-5	2.827770402009979E-4	9.695212806891358E-4
18	0.0	0.0	2.0198360014356995E-5	3.231737602297119E-4	0.0010705130807609207
19	0.0	0.0	2.0198360014356995E-5	3.231737602297119E-4	0.0010705130807609207
20	0.0	0.0	2.0198360014356995E-5	3.837688402727829E-4	0.0013330917609475617
21	0.0	0.0	2.0198360014356995E-5	4.241655603014969E-4	0.0013936868409906327
22	0.0	0.0	2.0198360014356995E-5	5.655540804019958E-4	0.0014744802810480605
23	0.0	0.0	2.0198360014356995E-5	8.079344005742798E-4	0.0014744802810480605
24	0.0	0.0	2.0198360014356995E-5	0.0012119016008614197	0.0014946786410624175
25	0.0	0.0	2.0198360014356995E-5	0.0014340835610193465	0.0014946786410624175
26	0.0	0.0	2.0198360014356995E-5	0.0017168606012203445	0.0015754720811198455
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5795	0.0	20.599094	1
GGTATCA	2155	0.0	18.637566	1
CTAGCGG	915	0.0	15.037757	29
CGTCGTA	1090	0.0	14.532368	10
TCTAGCG	965	0.0	14.258599	28
TATTCCG	135	1.3200588E-7	14.22348	5
TACCGTC	1155	0.0	14.131118	7
ACCGTCG	1135	0.0	14.098162	8
TAGCGGC	1000	0.0	13.919542	30
ATACCGT	1220	0.0	13.7717085	6
TAGGACG	940	0.0	13.447317	4
TTAGGAC	1030	0.0	13.359729	3
TAAACCG	120	8.719573E-6	13.334512	5
TATCAAC	9015	0.0	13.204359	2
TCAACGC	8960	0.0	13.107639	4
GTCGTAG	1235	0.0	12.955695	11
AACGCAG	9215	0.0	12.797658	6
CAACGCA	9245	0.0	12.738821	5
ATCAACG	9230	0.0	12.724209	3
GTCCTAC	1195	0.0	12.720918	1
>>END_MODULE