FastQCFastQC Report
Thu 2 Feb 2017
SRR4062725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415596
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50690.14840748144686902No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA38290.11210342206748106No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC37540.10990761202437291No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG37430.10958555988471703No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG36060.10557454687263951No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC35210.10308596215711693No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34770.1017977535984935No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC34380.10065593237607727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA550.00136090217.4631292
TCTAGCG9900.016.96859628
CTAGCGG10100.016.6330729
TAGCGGC10550.016.07525430
ACCGTCG6100.015.7362228
TACCGTC6300.015.4906037
ATACCGT7050.015.4300416
CGGTCCA11550.014.68388810
CGAGCCG9100.014.59331315
CGCGGTC5500.014.54536110
TAGGACG3300.014.5430184
AACCGCG5400.014.5171517
CGTCGTA6950.014.50350310
ATCGTTT6100.013.90114429
CGCCGGT11750.013.8880537
GTATCAA56900.013.8499171
AGCGGCG12300.013.78812631
AAGACGG9300.013.7613395
CAAGACG9100.013.711994
CATCGTT6350.013.60541528
Produced by FastQC (version 0.11.5)