##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062725_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3415596
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256083272143428	32.0	32.0	32.0	32.0	32.0
2	30.83499922121937	32.0	32.0	32.0	32.0	32.0
3	30.886711718833258	32.0	32.0	32.0	32.0	32.0
4	30.963812464940233	32.0	32.0	32.0	32.0	32.0
5	30.851990692107616	32.0	32.0	32.0	32.0	32.0
6	34.526811133401026	36.0	36.0	36.0	32.0	36.0
7	34.46494784511986	36.0	36.0	36.0	32.0	36.0
8	34.436996939919126	36.0	36.0	36.0	32.0	36.0
9	34.599374457634916	36.0	36.0	36.0	32.0	36.0
10	34.28710567643246	36.0	36.0	36.0	32.0	36.0
11	34.579648178531656	36.0	36.0	36.0	32.0	36.0
12	34.38810034910452	36.0	36.0	36.0	32.0	36.0
13	34.4729657722986	36.0	36.0	36.0	32.0	36.0
14	34.37421141141985	36.0	36.0	36.0	32.0	36.0
15	34.30584442656567	36.0	36.0	36.0	32.0	36.0
16	34.32901637078858	36.0	36.0	36.0	32.0	36.0
17	34.2575980882985	36.0	36.0	36.0	32.0	36.0
18	34.27709834535466	36.0	36.0	36.0	32.0	36.0
19	34.26840469423199	36.0	36.0	36.0	32.0	36.0
20	34.25270758017049	36.0	36.0	36.0	32.0	36.0
21	34.24366669828633	36.0	36.0	36.0	32.0	36.0
22	34.209965405744704	36.0	36.0	36.0	32.0	36.0
23	34.154771817275815	36.0	36.0	36.0	32.0	36.0
24	34.14192749962232	36.0	36.0	36.0	32.0	36.0
25	34.11344725781386	36.0	36.0	36.0	32.0	36.0
26	34.05972369097516	36.0	36.0	36.0	32.0	36.0
27	34.05862490762959	36.0	36.0	36.0	32.0	36.0
28	34.03893405426169	36.0	36.0	36.0	32.0	36.0
29	34.007401343718634	36.0	36.0	36.0	32.0	36.0
30	33.983301303784174	36.0	36.0	36.0	32.0	36.0
31	34.00060867854395	36.0	36.0	36.0	32.0	36.0
32	33.95930519885842	36.0	36.0	36.0	32.0	36.0
33	33.92733039856002	36.0	36.0	36.0	32.0	36.0
34	33.93388474515136	36.0	36.0	36.0	32.0	36.0
35	33.89428989845403	36.0	36.0	36.0	32.0	36.0
36	33.853062832957995	36.0	36.0	36.0	32.0	36.0
37	33.84850872292859	36.0	36.0	36.0	32.0	36.0
38	33.25733224889595	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	5.0
9	11.0
10	25.0
11	46.0
12	14.0
13	12.0
14	283.0
15	576.0
16	886.0
17	1087.0
18	1426.0
19	1929.0
20	2687.0
21	3954.0
22	6273.0
23	9819.0
24	15349.0
25	23547.0
26	33978.0
27	48923.0
28	68298.0
29	91931.0
30	123167.0
31	164824.0
32	223807.0
33	332182.0
34	731110.0
35	1529442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.470882485876366	17.00445941533413	11.21574905808261	26.308909040706897
2	16.836120293571856	19.353802124602534	37.645888836975644	26.16418874484997
3	18.80034832431875	23.572707723804818	28.434812755182115	29.192131196694316
4	12.451701814755726	15.311457967659795	36.212842566873796	36.02399765071069
5	14.668434640497999	36.46261578540256	33.8482766939553	15.02067288014414
6	34.573112283645955	35.48118592966001	16.80706245670577	13.138639329988273
7	30.548674535476252	30.24638459770552	21.063592141339942	18.141348725478284
8	28.15456665148138	33.11438616132859	19.40971803390807	19.32132915328195
9	27.44546187015311	14.316376194814836	18.378453866855292	39.859708068176765
10	15.51240300704155	26.575316717526697	31.974892719632344	25.937387555799408
11	37.74289070386775	21.014671816480497	22.205117334713695	19.037320144938057
12	24.812875840320324	23.49916396160694	28.840733441799227	22.847226756273507
13	29.398326142731733	19.31430532463707	25.255526475605734	26.03184205702546
14	23.483178177043897	19.746280377575275	25.10870309955359	31.66183834582724
15	25.32672484479325	27.13403420405295	22.170258873140448	25.368982078013353
16	26.10109153133548	25.54384777240116	23.570875163460318	24.784185532803036
17	24.24438445892088	25.866892656823133	25.16455835576934	24.72416452848665
18	25.140986455755243	24.68074740315846	26.044102021462205	24.134164119624092
19	25.57595476160569	25.208728061044777	25.21467185152587	24.000645325823662
20	25.640584201314166	24.385942721756003	24.944149993646423	25.029323083283412
21	26.779224209084852	24.332819285941618	24.278974602818604	24.60898190215493
22	25.61750806654682	24.46473850334081	24.801454613596306	25.11629881651607
23	24.278950113065964	24.14054203146288	25.451392762431475	26.129115093039683
24	24.74513161562662	25.22804629796644	24.98248951127582	25.044332575131122
25	25.037042546512556	24.523600640938543	25.03616406714862	25.403192745400283
26	24.72612377029858	25.318078861946937	25.46282454673634	24.49297282101814
27	25.46031203188483	24.95452804621098	24.526921856169253	25.058238065734933
28	24.61899632457464	24.715003014299697	25.397297582967905	25.268703078157763
29	24.606760325835523	25.11132518549318	25.382786467636052	24.89912802103525
30	24.664967741104356	25.073488680400953	25.614962646669937	24.646580931824758
31	25.13650658502086	24.94534319961656	24.554876832982814	25.363273382379763
32	24.681622049102305	24.978721124192216	24.638317670265412	25.701339156440067
33	24.46463660407865	24.570542474051305	25.2558123056355	25.709008616234545
34	25.172019104156657	24.735086626651125	25.43259155539119	24.660302713801027
35	25.82979897184341	24.601235006049702	25.19241333347389	24.376552688633
36	24.669238596516916	25.237483803430376	24.915616787332638	25.17766081272007
37	25.685036294486768	25.027955436442994	24.692460918753696	24.594547350316535
38	24.746632452946862	24.83608040536465	25.293977795826777	25.123309345861706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	166.5
2	237.0
3	237.0
4	634.0
5	1031.0
6	1031.0
7	1259.5
8	1488.0
9	1581.0
10	1674.0
11	1674.0
12	2225.0
13	2776.0
14	3893.5
15	5011.0
16	5011.0
17	7721.5
18	10432.0
19	10432.0
20	13549.0
21	16666.0
22	19981.5
23	23297.0
24	23297.0
25	28754.5
26	34212.0
27	34212.0
28	43666.5
29	53121.0
30	65130.0
31	77139.0
32	77139.0
33	98773.5
34	120408.0
35	120408.0
36	135316.0
37	150224.0
38	169968.5
39	189713.0
40	189713.0
41	203351.5
42	216990.0
43	244586.5
44	272183.0
45	272183.0
46	281574.5
47	290966.0
48	290966.0
49	307014.0
50	323062.0
51	323068.5
52	323075.0
53	323075.0
54	308772.5
55	294470.0
56	294470.0
57	283226.0
58	271982.0
59	243927.0
60	215872.0
61	215872.0
62	202115.0
63	188358.0
64	155973.5
65	123589.0
66	123589.0
67	102676.5
68	81764.0
69	81764.0
70	65350.5
71	48937.0
72	38187.0
73	27437.0
74	27437.0
75	20792.5
76	14148.0
77	14148.0
78	13530.0
79	12912.0
80	10118.5
81	7325.0
82	7325.0
83	7269.5
84	7214.0
85	7214.0
86	4845.5
87	2477.0
88	2139.0
89	1801.0
90	1801.0
91	1329.5
92	858.0
93	738.5
94	619.0
95	619.0
96	551.0
97	483.0
98	483.0
99	1016.0
100	1549.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11529466599679822
2	0.052143169157008035
3	0.011359657289679458
4	0.002781359387936981
5	2.9277467241441905E-4
6	4.098845413801867E-4
7	2.9277467241441902E-5
8	2.3421973793153521E-4
9	1.1710986896576761E-4
10	3.8060707413874477E-4
11	0.001990867772418049
12	0.002430029781039678
13	0.014345958948306533
14	0.009661564189675828
15	0.023304863924187756
16	0.01153532209312811
17	0.020435672134526447
18	0.005767661046564055
19	0.007817083753464989
20	0.005796938513805496
21	0.0063532103913928935
22	0.007495031613809127
23	0.009749396591400155
24	0.014638733620720953
25	0.01768359021383091
26	0.01876685650176426
27	0.00890035004139834
28	0.006118990653461358
29	0.011008327682782156
30	0.003044856593109958
31	0.005650551177598288
32	0.006938759736221731
33	0.00875396270519113
34	0.012384368643129926
35	0.01616116191727593
36	0.015253560432791231
37	0.009925061394848805
38	0.005914048382771265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3415596.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.09167199536324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09133766753314	38.11542328130497
2	13.612266453957014	13.63722372650202
3	4.568991518868063	6.866052735382065
4	1.9491796765132425	3.9055067606371825
5	1.0222046164696712	2.5601969180172426
6	0.6648355522427661	1.9981634648280493
7	0.39973722227266484	1.4016454075693956
8	0.2925746562412956	1.1724442971676108
9	0.2129844263741306	0.9601871423448187
>10	1.0318412078927026	9.130957203485606
>50	0.07034769827523757	2.470308741214925
>100	0.06432717168687391	6.901926724190711
>500	0.011717489980062184	4.059266176915834
>1k	0.0075957598339227396	6.671187732983533
>5k	5.888185917769566E-5	0.14950968745603532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5069	0.14840748144686902	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3829	0.11210342206748106	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3754	0.10990761202437291	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3743	0.10958555988471703	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3606	0.10557454687263951	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3521	0.10308596215711693	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3477	0.1017977535984935	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3438	0.10065593237607727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9277467241441902E-5
2	0.0	0.0	0.0	0.0	2.9277467241441902E-5
3	0.0	0.0	0.0	0.0	2.9277467241441902E-5
4	0.0	0.0	0.0	0.0	2.9277467241441902E-5
5	0.0	0.0	0.0	0.0	2.9277467241441902E-5
6	0.0	0.0	0.0	0.0	2.9277467241441902E-5
7	0.0	0.0	0.0	5.8554934482883804E-5	2.9277467241441902E-5
8	0.0	0.0	0.0	8.783240172432572E-5	2.9277467241441902E-5
9	0.0	0.0	0.0	1.4638733620720953E-4	5.8554934482883804E-5
10	0.0	0.0	0.0	1.7566480344865143E-4	5.8554934482883804E-5
11	0.0	0.0	0.0	1.7566480344865143E-4	5.8554934482883804E-5
12	0.0	0.0	0.0	1.7566480344865143E-4	1.7566480344865143E-4
13	0.0	0.0	0.0	1.7566480344865143E-4	1.7566480344865143E-4
14	0.0	0.0	0.0	1.7566480344865143E-4	2.0494227069009334E-4
15	0.0	0.0	0.0	2.0494227069009334E-4	2.9277467241441905E-4
16	0.0	0.0	0.0	2.6349720517297715E-4	2.9277467241441905E-4
17	0.0	0.0	0.0	2.9277467241441905E-4	2.9277467241441905E-4
18	0.0	0.0	0.0	4.391620086216286E-4	2.9277467241441905E-4
19	0.0	0.0	0.0	4.977169431045124E-4	2.9277467241441905E-4
20	0.0	0.0	0.0	5.855493448288381E-4	3.2205213965586096E-4
21	0.0	0.0	0.0	6.1482681207028E-4	3.5132960689730286E-4
22	0.0	0.0	0.0	7.612141482774895E-4	4.098845413801867E-4
23	0.0	0.0	0.0	0.0011125437551747924	4.098845413801867E-4
24	0.0	0.0	0.0	0.0016395381655207467	4.098845413801867E-4
25	0.0	0.0	0.0	0.002195810043108143	4.098845413801867E-4
26	0.0	0.0	0.0	0.0028984692569027485	4.098845413801867E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATA	55	0.001360902	17.463129	2
TCTAGCG	990	0.0	16.968596	28
CTAGCGG	1010	0.0	16.63307	29
TAGCGGC	1055	0.0	16.075254	30
ACCGTCG	610	0.0	15.736222	8
TACCGTC	630	0.0	15.490603	7
ATACCGT	705	0.0	15.430041	6
CGGTCCA	1155	0.0	14.683888	10
CGAGCCG	910	0.0	14.593313	15
CGCGGTC	550	0.0	14.545361	10
TAGGACG	330	0.0	14.543018	4
AACCGCG	540	0.0	14.517151	7
CGTCGTA	695	0.0	14.503503	10
ATCGTTT	610	0.0	13.901144	29
CGCCGGT	1175	0.0	13.888053	7
GTATCAA	5690	0.0	13.849917	1
AGCGGCG	1230	0.0	13.788126	31
AAGACGG	930	0.0	13.761339	5
CAAGACG	910	0.0	13.71199	4
CATCGTT	635	0.0	13.605415	28
>>END_MODULE