FastQCFastQC Report
Thu 2 Feb 2017
SRR4062725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415596
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51160.1497835224072168No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC42960.1257759992692344No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA38730.1133916306261045No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC38590.11298174608472432No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG37810.11069810363989183No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT36110.1057209342088467No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA35600.10422778337953317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35280.10329090442780704No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35210.10308596215711693No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG35010.1025004128122881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG9300.018.7519529
TCTAGCG9650.018.07182728
TATAACG450.00885351317.7778592
TAGCGGC10000.017.43931430
ATCGTTT5450.017.3204229
CATCGTT5800.016.27522328
AGCGGCG11350.015.50599931
TACCGTC6550.015.3911877
ATACCGT7200.015.1129516
CGTCGTA6150.015.08994210
ACCGTCG6350.014.8677338
CGTTTAT6650.014.67611431
CAAGACG10250.014.6743114
GCATCGT7100.014.64731127
AAGACGG10300.014.4485715
GCGGCGC12200.014.16337832
CGGCATC6700.014.08899925
GTATTAG8100.014.0253721
CGGTCCA12800.014.00047410
GCGTAAC4400.013.81844611
Produced by FastQC (version 0.11.5)