##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3415596
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203078174350832	32.0	32.0	32.0	32.0	32.0
2	31.25617959501065	32.0	32.0	32.0	32.0	32.0
3	31.362625439308395	32.0	32.0	32.0	32.0	32.0
4	31.468398780183605	32.0	32.0	32.0	32.0	32.0
5	31.384657318956926	32.0	32.0	32.0	32.0	32.0
6	34.91447378437028	36.0	36.0	36.0	36.0	36.0
7	34.95072543708331	36.0	36.0	36.0	36.0	36.0
8	34.874835021472094	36.0	36.0	36.0	32.0	36.0
9	34.996109610152956	36.0	36.0	36.0	36.0	36.0
10	34.83799752663957	36.0	36.0	36.0	32.0	36.0
11	35.02827998393253	36.0	36.0	36.0	36.0	36.0
12	34.91982746203005	36.0	36.0	36.0	32.0	36.0
13	34.97617077663752	36.0	36.0	36.0	36.0	36.0
14	34.921977891998935	36.0	36.0	36.0	32.0	36.0
15	34.88597656163083	36.0	36.0	36.0	32.0	36.0
16	34.90273878995057	36.0	36.0	36.0	32.0	36.0
17	34.871786651582916	36.0	36.0	36.0	32.0	36.0
18	34.87163675095064	36.0	36.0	36.0	32.0	36.0
19	34.8712052010835	36.0	36.0	36.0	32.0	36.0
20	34.85572591137828	36.0	36.0	36.0	32.0	36.0
21	34.848800326502314	36.0	36.0	36.0	32.0	36.0
22	34.82968887421112	36.0	36.0	36.0	32.0	36.0
23	34.77058469444278	36.0	36.0	36.0	32.0	36.0
24	34.74321319031876	36.0	36.0	36.0	32.0	36.0
25	34.72768559279259	36.0	36.0	36.0	32.0	36.0
26	34.66285591153052	36.0	36.0	36.0	32.0	36.0
27	34.64693336097126	36.0	36.0	36.0	32.0	36.0
28	34.61534824376185	36.0	36.0	36.0	32.0	36.0
29	34.58112522675398	36.0	36.0	36.0	32.0	36.0
30	34.558232589568554	36.0	36.0	36.0	32.0	36.0
31	34.5562092238075	36.0	36.0	36.0	32.0	36.0
32	34.53015022853991	36.0	36.0	36.0	32.0	36.0
33	34.50464691960056	36.0	36.0	36.0	32.0	36.0
34	34.50064029820857	36.0	36.0	36.0	32.0	36.0
35	34.468687456010606	36.0	36.0	36.0	32.0	36.0
36	34.439504847763025	36.0	36.0	36.0	32.0	36.0
37	34.43579773486091	36.0	36.0	36.0	32.0	36.0
38	34.00356658105935	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	18.0
21	70.0
22	232.0
23	779.0
24	2182.0
25	5229.0
26	11315.0
27	22142.0
28	38468.0
29	61361.0
30	93213.0
31	136390.0
32	197313.0
33	318466.0
34	778877.0
35	1749537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85921337418485	18.18896517315044	11.903799846338355	27.048021606326362
2	15.793700425928595	20.34863022441764	36.90254936473751	26.955119984916248
3	17.766708943329363	24.378790700071086	28.7150470957338	29.139453260865743
4	11.9736525831108	16.221819298622112	36.19716177961588	35.607366338651204
5	14.172272332292616	37.03314096062996	33.47320171730113	15.321384989776297
6	34.589640683079246	35.92794455543871	16.45878664664866	13.023628114833388
7	30.372308111202035	30.4377434612226	21.014350396784153	18.175598030791214
8	28.053360019393846	33.38151541377643	19.14720938212557	19.417915184704157
9	27.26512550384676	14.109670900633676	18.221341524292537	40.40386207122703
10	15.243920112777829	26.874206756422435	32.018831437844106	25.86304169295563
11	37.91219505339585	20.917019623274815	22.09476160277142	19.076023720557913
12	24.586834930717558	23.64802036781997	28.726127069338652	23.03901763212382
13	29.412370922739555	19.316311383977585	25.202175905187683	26.069141788095173
14	23.406720232720733	19.684997874455878	25.01762503527935	31.89065685754404
15	25.289934758092	27.180175875601215	22.025994877614334	25.50389448869246
16	25.9771214026023	25.65196566102411	23.475594581791633	24.895318354581956
17	24.25280975853116	25.91978091085714	25.046668282782857	24.78074104782884
18	25.182720673053836	24.743705051768416	25.90028211767434	24.173292157503404
19	25.547781412087378	25.247043268583287	25.12940640520717	24.07576891412216
20	25.656312983151402	24.389389143212487	24.826531006594458	25.12776686704165
21	26.694134810558516	24.425187661410476	24.24076873327706	24.639908794753943
22	25.49472375660683	24.58603820771386	24.817828119451	25.101409916228306
23	24.20425168126151	24.104649562740256	25.438357648312593	26.25274110768564
24	24.758285230454653	25.236152050769473	24.86705102125661	25.138511697519263
25	24.95154579171541	24.590349678357747	25.063649213782895	25.394455316143947
26	24.80038599458015	25.233839404706416	25.370711724351153	24.59506287636228
27	25.576898866667253	24.880064644761227	24.43557335628691	25.107463132284614
28	24.548746397407655	24.70312648217178	25.45476689866132	25.293360221759247
29	24.626448795466445	24.98249207458962	25.38745800147324	25.0036011284707
30	24.682105260692424	24.94027396682746	25.62229256621685	24.75532820626327
31	25.079459046093273	24.803021200399577	24.575154672859437	25.542365080647713
32	24.750585256570158	24.927977430586054	24.588534475388776	25.73290283745501
33	24.421623634645314	24.525207313745536	25.27432986805231	25.77883918355684
34	25.097845295520898	24.659181003842377	25.447066924776816	24.79590677585991
35	25.909972959331256	24.540402319243846	25.129142908002	24.4204818134229
36	24.746720630894288	25.144542855770997	24.91881358333948	25.189922929995234
37	25.733546941734325	24.944050760101604	24.674141789602754	24.648260508561318
38	24.75480845943386	24.734958330832548	25.27208290210051	25.238150307633077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	85.5
2	122.0
3	122.0
4	489.0
5	856.0
6	856.0
7	1089.0
8	1322.0
9	1459.5
10	1597.0
11	1597.0
12	2077.5
13	2558.0
14	3805.5
15	5053.0
16	5053.0
17	7663.5
18	10274.0
19	10274.0
20	13421.5
21	16569.0
22	19904.5
23	23240.0
24	23240.0
25	28792.5
26	34345.0
27	34345.0
28	43884.5
29	53424.0
30	65311.0
31	77198.0
32	77198.0
33	98928.0
34	120658.0
35	120658.0
36	135230.0
37	149802.0
38	169370.0
39	188938.0
40	188938.0
41	203367.5
42	217797.0
43	245504.0
44	273211.0
45	273211.0
46	282452.5
47	291694.0
48	291694.0
49	308139.5
50	324585.0
51	324384.5
52	324184.0
53	324184.0
54	309060.5
55	293937.0
56	293937.0
57	283778.0
58	273619.0
59	245354.5
60	217090.0
61	217090.0
62	203101.5
63	189113.0
64	156230.5
65	123348.0
66	123348.0
67	102540.0
68	81732.0
69	81732.0
70	65511.5
71	49291.0
72	38461.0
73	27631.0
74	27631.0
75	20731.0
76	13831.0
77	13831.0
78	13141.5
79	12452.0
80	9675.0
81	6898.0
82	6898.0
83	6502.5
84	6107.0
85	6107.0
86	3893.5
87	1680.0
88	1360.5
89	1041.0
90	1041.0
91	624.5
92	208.0
93	138.0
94	68.0
95	68.0
96	53.5
97	39.0
98	39.0
99	37.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008783240172432571
2	0.0
3	0.0
4	1.4638733620720953E-4
5	1.1710986896576761E-4
6	1.1710986896576761E-4
7	3.2205213965586096E-4
8	0.0023421973793153524
9	0.0036011284706973542
10	9.07601484484699E-4
11	0.004742949693113589
12	3.513296068973028E-4
13	1.756648034486514E-4
14	0.0
15	0.0
16	5.8554934482883804E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	8.78324017243257E-5
22	8.78324017243257E-5
23	1.756648034486514E-4
24	0.0
25	0.0
26	1.1710986896576761E-4
27	1.756648034486514E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.9277467241441902E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3415596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.85706907779692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94663003187726	35.38868981505842
2	14.873993590644469	14.23651477460362
3	4.915781717868011	7.057647157301419
4	2.173623023570119	4.160929087523399
5	1.135217267667159	2.716408559852755
6	0.6897396862496461	1.9805351886328377
7	0.43551895117931555	1.458986237189472
8	0.3115910824358828	1.1929468764927644
9	0.2250821840208958	0.969459626598247
>10	1.124905082312835	9.566056616727927
>50	0.07776389315678421	2.5769090475420744
>100	0.06828296261941531	6.93424043457358
>500	0.01361517277305057	4.500841341884973
>1k	0.008193746508594384	7.108998364462956
>5k	6.16071166059728E-5	0.1508368715555078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5116	0.1497835224072168	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4296	0.1257759992692344	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3873	0.1133916306261045	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3859	0.11298174608472432	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3781	0.11069810363989183	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3611	0.1057209342088467	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3560	0.10422778337953317	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3528	0.10329090442780704	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3521	0.10308596215711693	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3501	0.1025004128122881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9277467241441902E-5
2	0.0	0.0	0.0	0.0	2.9277467241441902E-5
3	0.0	0.0	0.0	0.0	2.9277467241441902E-5
4	0.0	0.0	0.0	0.0	2.9277467241441902E-5
5	2.9277467241441902E-5	0.0	0.0	0.0	5.8554934482883804E-5
6	2.9277467241441902E-5	0.0	0.0	0.0	8.783240172432572E-5
7	2.9277467241441902E-5	0.0	0.0	2.9277467241441902E-5	8.783240172432572E-5
8	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	8.783240172432572E-5
9	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	8.783240172432572E-5
10	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	8.783240172432572E-5
11	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	8.783240172432572E-5
12	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	1.4638733620720953E-4
13	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	1.7566480344865143E-4
14	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	2.0494227069009334E-4
15	2.9277467241441902E-5	0.0	0.0	5.8554934482883804E-5	2.3421973793153521E-4
16	5.8554934482883804E-5	0.0	0.0	1.4638733620720953E-4	2.6349720517297715E-4
17	5.8554934482883804E-5	0.0	0.0	1.4638733620720953E-4	2.6349720517297715E-4
18	5.8554934482883804E-5	0.0	0.0	3.2205213965586096E-4	2.6349720517297715E-4
19	5.8554934482883804E-5	0.0	0.0	3.8060707413874477E-4	2.6349720517297715E-4
20	5.8554934482883804E-5	0.0	0.0	4.6843947586307043E-4	2.6349720517297715E-4
21	5.8554934482883804E-5	0.0	0.0	5.269944103459543E-4	2.9277467241441905E-4
22	5.8554934482883804E-5	2.9277467241441902E-5	0.0	6.441042793117219E-4	3.2205213965586096E-4
23	5.8554934482883804E-5	2.9277467241441902E-5	0.0	9.076014844846991E-4	3.5132960689730286E-4
24	5.8554934482883804E-5	2.9277467241441902E-5	0.0	0.0013174860258648856	3.5132960689730286E-4
25	5.8554934482883804E-5	2.9277467241441902E-5	0.0	0.0017273705672450723	3.5132960689730286E-4
26	5.8554934482883804E-5	2.9277467241441902E-5	0.0	0.0023129199120739104	3.5132960689730286E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	930	0.0	18.75195	29
TCTAGCG	965	0.0	18.071827	28
TATAACG	45	0.008853513	17.777859	2
TAGCGGC	1000	0.0	17.439314	30
ATCGTTT	545	0.0	17.32042	29
CATCGTT	580	0.0	16.275223	28
AGCGGCG	1135	0.0	15.505999	31
TACCGTC	655	0.0	15.391187	7
ATACCGT	720	0.0	15.112951	6
CGTCGTA	615	0.0	15.089942	10
ACCGTCG	635	0.0	14.867733	8
CGTTTAT	665	0.0	14.676114	31
CAAGACG	1025	0.0	14.674311	4
GCATCGT	710	0.0	14.647311	27
AAGACGG	1030	0.0	14.448571	5
GCGGCGC	1220	0.0	14.163378	32
CGGCATC	670	0.0	14.088999	25
GTATTAG	810	0.0	14.025372	1
CGGTCCA	1280	0.0	14.000474	10
GCGTAAC	440	0.0	13.818446	11
>>END_MODULE