FastQCFastQC Report
Thu 2 Feb 2017
SRR4062724_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062724_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230743
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27200.22100471016288534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19300.15681584213763555No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13020.10578975464414586No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12790.10392096481556264No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12610.10245843364536708No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12480.10140216113355917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG1101.0628355E-817.45523315
CGTTTAT2700.015.9999831
CGCTTCG3100.015.9999832
TCTAGCG3500.015.99867828
GGATATA1153.300902E-715.3223881
TTGCACC1052.1907108E-615.2343614
CTAGCGG3700.015.13511729
GTAAACG3400.015.0575827
CGTCTTA650.004160710614.76981315
TAGGACG3600.014.6630734
AACGCTT3450.014.37679330
TTAGGAC2450.014.3644093
CGGTCCA4250.014.30644610
GTATCAA22600.014.2468621
AGTAAAC3600.014.22220426
TAGCGGC4050.014.22220330
TGCACCG901.5391149E-414.2170045
AAACGCT3500.014.1714129
TATATGA1258.559673E-714.0845622
TCGTTTA3100.013.93546730
Produced by FastQC (version 0.11.5)