##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062724_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1230743
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24879849001782	32.0	32.0	32.0	32.0	32.0
2	30.87336348855935	32.0	32.0	32.0	32.0	32.0
3	30.92904611279528	32.0	32.0	32.0	32.0	32.0
4	30.99149863131458	32.0	32.0	32.0	32.0	32.0
5	30.908267607453382	32.0	32.0	32.0	32.0	32.0
6	34.55966599038142	36.0	36.0	36.0	32.0	36.0
7	34.50505995159022	36.0	36.0	36.0	32.0	36.0
8	34.486335490025134	36.0	36.0	36.0	32.0	36.0
9	34.61393727203811	36.0	36.0	36.0	32.0	36.0
10	34.3480369175368	36.0	36.0	36.0	32.0	36.0
11	34.602595342813245	36.0	36.0	36.0	32.0	36.0
12	34.43426450526227	36.0	36.0	36.0	32.0	36.0
13	34.51125539613063	36.0	36.0	36.0	32.0	36.0
14	34.41363225303739	36.0	36.0	36.0	32.0	36.0
15	34.3660837396597	36.0	36.0	36.0	32.0	36.0
16	34.377040535676414	36.0	36.0	36.0	32.0	36.0
17	34.311816520589595	36.0	36.0	36.0	32.0	36.0
18	34.32421309729163	36.0	36.0	36.0	32.0	36.0
19	34.32247106016447	36.0	36.0	36.0	32.0	36.0
20	34.30190868442884	36.0	36.0	36.0	32.0	36.0
21	34.27859187498934	36.0	36.0	36.0	32.0	36.0
22	34.259173523635724	36.0	36.0	36.0	32.0	36.0
23	34.21563640825095	36.0	36.0	36.0	32.0	36.0
24	34.19511222082921	36.0	36.0	36.0	32.0	36.0
25	34.16761419727758	36.0	36.0	36.0	32.0	36.0
26	34.12583455684899	36.0	36.0	36.0	32.0	36.0
27	34.1357448305617	36.0	36.0	36.0	32.0	36.0
28	34.11387023935948	36.0	36.0	36.0	32.0	36.0
29	34.073747321739795	36.0	36.0	36.0	32.0	36.0
30	34.04869010020776	36.0	36.0	36.0	32.0	36.0
31	34.07548529628038	36.0	36.0	36.0	32.0	36.0
32	34.016688293169246	36.0	36.0	36.0	32.0	36.0
33	33.97982275747252	36.0	36.0	36.0	32.0	36.0
34	33.98927070883198	36.0	36.0	36.0	32.0	36.0
35	33.954359277282094	36.0	36.0	36.0	32.0	36.0
36	33.905873931438165	36.0	36.0	36.0	32.0	36.0
37	33.904838784376594	36.0	36.0	36.0	32.0	36.0
38	33.309565847622125	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	3.0
8	4.0
9	2.0
10	8.0
11	5.0
12	6.0
13	6.0
14	149.0
15	279.0
16	362.0
17	452.0
18	589.0
19	807.0
20	1030.0
21	1529.0
22	2252.0
23	3434.0
24	5117.0
25	7809.0
26	11426.0
27	16305.0
28	23085.0
29	31580.0
30	42608.0
31	58039.0
32	79028.0
33	118127.0
34	264751.0
35	561948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.02019094352393	17.457275247627848	11.652338309752826	25.870195499095395
2	16.392135247980647	19.743824872205096	38.26449317776824	25.599546702046016
3	18.563317538857092	24.204472781141543	28.905923050481917	28.326286629519444
4	12.406476901175905	15.698148713915197	36.338802254304824	35.556572130604074
5	14.461299705868013	36.69743406405902	33.8118530315095	15.029413198563466
6	33.70148642521804	35.847272124530164	17.161816731099552	13.28942471915225
7	29.85678558138099	30.558963617070027	21.366622736528047	18.21762806502094
8	27.77635134805734	33.28420276030401	19.675656965680645	19.263788925958007
9	27.64763905884112	14.084557395643916	18.666279906852637	39.601523638662336
10	15.795315308288076	26.72385214652743	31.94237250676833	25.53846003841616
11	37.15036726469059	21.068317732709307	22.64739339573583	19.133921606864273
12	24.660927983151215	23.913961999232964	29.004348702214656	22.420761315401165
13	29.614123478861508	19.59173557076976	25.191719318204903	25.602421632163825
14	23.471393502767242	19.984528463483027	25.408504632116273	31.135573401633447
15	25.056136948961903	27.4580588115302	22.356931033838208	25.128873205669688
16	25.53253384862663	25.641259001516303	24.180945307510164	24.6452618423469
17	23.89862381816911	25.991340147874126	25.475373545517048	24.634662488439716
18	24.78328983420494	24.806854119199528	26.612528399026225	23.797327647569304
19	25.626865889947233	24.86693980234771	25.546014044656225	23.960180263048837
20	25.759076537796798	24.330264822580816	25.22627862966036	24.68438000996203
21	26.87700754630205	24.285002709908188	24.504233063480108	24.333756680309655
22	25.801337655130048	24.599869501024248	24.924735568934093	24.67405727491161
23	24.15728739297227	24.406751289370806	25.731753991219197	25.70420732643773
24	24.80679873425745	24.961441009213527	25.354832103833797	24.87692815269523
25	24.79584315618476	24.646885252029787	25.503088458891117	25.05418313289434
26	24.493613742987794	25.55258574942728	25.77273425418091	24.181066253404012
27	25.160831603196726	25.06258963868247	24.920549147391387	24.856029610729422
28	24.317081534183707	24.912527606823577	25.708595452573142	25.061795406419574
29	24.61881126972983	24.964164521413526	25.577593155992407	24.83943105286424
30	24.360614018551864	25.1295185226113	25.92556178862317	24.584305670213666
31	25.08474989436734	24.889573244060195	24.896154971235415	25.12952189033705
32	24.73487954093187	25.06258801121668	24.737073473165363	25.465458974686083
33	24.198121141746455	24.76749921382434	25.542379946710696	25.491999697718505
34	24.95888171260105	24.91768216376455	25.658786474196244	24.464649649438158
35	25.512570679088093	24.720737354152007	25.50249398242429	24.26419798433561
36	24.382383223479717	25.369156095067595	25.272290965945242	24.97616971550744
37	25.275185149671792	25.138100446439456	24.987526673416617	24.59918773047213
38	24.47390103862799	25.198064131337443	25.348145957216524	24.979888872818044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	440.0
1	347.0
2	254.0
3	254.0
4	503.5
5	753.0
6	753.0
7	842.5
8	932.0
9	906.0
10	880.0
11	880.0
12	1205.5
13	1531.0
14	2033.0
15	2535.0
16	2535.0
17	3788.0
18	5041.0
19	5041.0
20	6239.5
21	7438.0
22	8390.5
23	9343.0
24	9343.0
25	11200.5
26	13058.0
27	13058.0
28	16665.0
29	20272.0
30	25217.0
31	30162.0
32	30162.0
33	37712.5
34	45263.0
35	45263.0
36	50299.0
37	55335.0
38	62629.0
39	69923.0
40	69923.0
41	74570.0
42	79217.0
43	88288.5
44	97360.0
45	97360.0
46	100312.0
47	103264.0
48	103264.0
49	109614.0
50	115964.0
51	115435.5
52	114907.0
53	114907.0
54	109503.5
55	104100.0
56	104100.0
57	100457.5
58	96815.0
59	86271.0
60	75727.0
61	75727.0
62	70784.5
63	65842.0
64	53959.5
65	42077.0
66	42077.0
67	35511.5
68	28946.0
69	28946.0
70	22976.5
71	17007.0
72	13273.5
73	9540.0
74	9540.0
75	7192.0
76	4844.0
77	4844.0
78	4577.0
79	4310.0
80	3399.0
81	2488.0
82	2488.0
83	2504.0
84	2520.0
85	2520.0
86	1675.5
87	831.0
88	724.0
89	617.0
90	617.0
91	456.5
92	296.0
93	264.5
94	233.0
95	233.0
96	206.0
97	179.0
98	179.0
99	339.0
100	499.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1201713111510689
2	0.0525698703953628
3	0.011700249361564519
4	0.002518803682003473
5	2.4375519503259415E-4
6	4.062586583876569E-4
7	8.125173167753137E-5
8	7.312655850977824E-4
9	4.062586583876569E-4
10	8.937690484528451E-4
11	0.0018687898285832216
12	0.002518803682003473
13	0.013650290921825271
14	0.007800166241043013
15	0.021937967552933473
16	0.00950645260627117
17	0.019256660407574934
18	0.005443866022394603
19	0.007881417972720543
20	0.005687621217427196
21	0.006012628144137322
22	0.0066626419975575725
23	0.008450180094463263
24	0.013081528800082553
25	0.015762835945441086
26	0.017062863652281587
27	0.008775187021173388
28	0.005606369485749665
29	0.009668956069626233
30	0.002518803682003473
31	0.005118859095684477
32	0.006175131607492384
33	0.008287676631108201
34	0.011944004556597113
35	0.01470656343363318
36	0.013487787458470209
37	0.00950645260627117
38	0.0065813902658800405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1230743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.97910691489962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13698471627082	49.265029879646825
2	11.327705859454333	13.588513616894925
3	3.0465975536878833	5.481966011979516
4	1.1700782261714648	2.807209881053375
5	0.619017492913316	1.856405819482045
6	0.3837807174284933	1.3811294809512287
7	0.24581916074347307	1.0320809606774568
8	0.16652749531014768	0.7990536356382234
9	0.12114292663545584	0.6539440093786661
>10	0.6352129823327426	6.904330795047098
>50	0.06795362113737743	2.8543633807367255
>100	0.06965597992966577	8.694767424379945
>500	0.007210474331459305	2.899967720607581
>1k	0.0023127936534869465	1.781237383526348
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2720	0.22100471016288534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1930	0.15681584213763555	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1302	0.10578975464414586	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1279	0.10392096481556264	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1261	0.10245843364536708	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1248	0.10140216113355917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	1.6250346335506275E-4
16	0.0	0.0	0.0	1.6250346335506275E-4	2.4375519503259412E-4
17	0.0	0.0	0.0	2.4375519503259412E-4	2.4375519503259412E-4
18	0.0	0.0	0.0	2.4375519503259412E-4	2.4375519503259412E-4
19	0.0	0.0	0.0	2.4375519503259412E-4	2.4375519503259412E-4
20	0.0	0.0	0.0	2.4375519503259412E-4	2.4375519503259412E-4
21	0.0	0.0	0.0	3.250069267101255E-4	2.4375519503259412E-4
22	0.0	0.0	0.0	4.062586583876569E-4	2.4375519503259412E-4
23	0.0	0.0	0.0	0.001300027706840502	2.4375519503259412E-4
24	0.0	0.0	0.0	0.002112545023615816	2.4375519503259412E-4
25	0.0	0.0	0.0	0.002112545023615816	2.4375519503259412E-4
26	0.0	0.0	0.0	0.00235630021864841	4.062586583876569E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	110	1.0628355E-8	17.455233	15
CGTTTAT	270	0.0	15.99998	31
CGCTTCG	310	0.0	15.99998	32
TCTAGCG	350	0.0	15.998678	28
GGATATA	115	3.300902E-7	15.322388	1
TTGCACC	105	2.1907108E-6	15.234361	4
CTAGCGG	370	0.0	15.135117	29
GTAAACG	340	0.0	15.05758	27
CGTCTTA	65	0.0041607106	14.769813	15
TAGGACG	360	0.0	14.663073	4
AACGCTT	345	0.0	14.376793	30
TTAGGAC	245	0.0	14.364409	3
CGGTCCA	425	0.0	14.306446	10
GTATCAA	2260	0.0	14.246862	1
AGTAAAC	360	0.0	14.222204	26
TAGCGGC	405	0.0	14.222203	30
TGCACCG	90	1.5391149E-4	14.217004	5
AAACGCT	350	0.0	14.17141	29
TATATGA	125	8.559673E-7	14.084562	2
TCGTTTA	310	0.0	13.935467	30
>>END_MODULE