FastQCFastQC Report
Thu 2 Feb 2017
SRR4062724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230743
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30350.24659900564130774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21380.17371620232656207No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13810.11220864144667084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13220.10741478927769649No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13140.10676477542427623No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12750.10359595788885251No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12500.10156466459691421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA450.00885398917.77660414
GGTATAT652.099659E-417.2317311
TACCGTC2850.016.8457837
GTATAGG957.6889773E-716.8430461
GCATTCG4700.015.99894322
AGGGGCG3150.015.74499232
ATACCGT3050.015.7411436
TCTAGCG2950.015.72777528
GCGAGAA2650.015.69707720
CGTATTG4950.015.51412727
ACCGTCG2900.015.4516518
ATTATAC1255.1339157E-815.3608583
AGGGACG5250.015.2414258
CTAGCGG3050.015.21211129
GCTATAT756.242151E-414.9341681
GTAGGAC4000.014.8008263
TGTAGGA4350.014.7128682
CCGATAA1205.4087104E-714.6686779
CGGGGGC5350.014.65323917
ATTCGTA5250.014.62760524
Produced by FastQC (version 0.11.5)