##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1230743
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17027681652465	32.0	32.0	32.0	32.0	32.0
2	31.23315834418721	32.0	32.0	32.0	32.0	32.0
3	31.333299478445134	32.0	32.0	32.0	32.0	32.0
4	31.44531230321846	32.0	32.0	32.0	32.0	32.0
5	31.350732849993864	32.0	32.0	32.0	32.0	32.0
6	34.87658105713378	36.0	36.0	36.0	36.0	36.0
7	34.907143896004285	36.0	36.0	36.0	36.0	36.0
8	34.83629482353343	36.0	36.0	36.0	32.0	36.0
9	34.97639393439572	36.0	36.0	36.0	36.0	36.0
10	34.795055507120495	36.0	36.0	36.0	32.0	36.0
11	34.986943659236736	36.0	36.0	36.0	36.0	36.0
12	34.88187216990062	36.0	36.0	36.0	32.0	36.0
13	34.94239820986185	36.0	36.0	36.0	36.0	36.0
14	34.88415128097417	36.0	36.0	36.0	32.0	36.0
15	34.84943729113227	36.0	36.0	36.0	32.0	36.0
16	34.86306889415581	36.0	36.0	36.0	32.0	36.0
17	34.82763257641928	36.0	36.0	36.0	32.0	36.0
18	34.83391414779528	36.0	36.0	36.0	32.0	36.0
19	34.824779828120086	36.0	36.0	36.0	32.0	36.0
20	34.81423660341761	36.0	36.0	36.0	32.0	36.0
21	34.80353737539031	36.0	36.0	36.0	32.0	36.0
22	34.78216735744181	36.0	36.0	36.0	32.0	36.0
23	34.731327336413855	36.0	36.0	36.0	32.0	36.0
24	34.71294006953523	36.0	36.0	36.0	32.0	36.0
25	34.68466446691145	36.0	36.0	36.0	32.0	36.0
26	34.62833345385673	36.0	36.0	36.0	32.0	36.0
27	34.602109457457814	36.0	36.0	36.0	32.0	36.0
28	34.57321390412133	36.0	36.0	36.0	32.0	36.0
29	34.53518728117893	36.0	36.0	36.0	32.0	36.0
30	34.52098610351633	36.0	36.0	36.0	32.0	36.0
31	34.51194603584989	36.0	36.0	36.0	32.0	36.0
32	34.48053086631409	36.0	36.0	36.0	32.0	36.0
33	34.45562883558956	36.0	36.0	36.0	32.0	36.0
34	34.44194766900969	36.0	36.0	36.0	32.0	36.0
35	34.41312199216246	36.0	36.0	36.0	32.0	36.0
36	34.38521608491781	36.0	36.0	36.0	32.0	36.0
37	34.37331433126168	36.0	36.0	36.0	32.0	36.0
38	33.92801502832029	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	6.0
21	26.0
22	76.0
23	293.0
24	822.0
25	1968.0
26	4219.0
27	8412.0
28	14655.0
29	23067.0
30	35159.0
31	51241.0
32	73430.0
33	118040.0
34	285893.0
35	613432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40444973529925	18.726673627842537	12.343464959147108	26.5254116777111
2	15.524199609504178	20.868532260593803	37.42202880698895	26.185239322913066
3	17.539567562033668	25.15480486177862	29.115745529326592	28.189882046861126
4	11.965939052679902	16.670363644488862	36.31594128711705	35.047756015714185
5	13.987648111750381	37.321520414903844	33.45921934961239	15.231612123733388
6	33.65565947668925	36.42041664330676	16.72569614565534	13.198227734348656
7	29.602798327828495	30.807363079785656	21.354312666821045	18.235525925564804
8	27.576165074773606	33.72779238085178	19.381741507985197	19.314301036389416
9	27.584604221496527	13.938153946409404	18.45281672802736	40.02442510406671
10	15.53232634290218	26.981385031647886	31.97427543002933	25.5120131954206
11	37.35319115742328	20.980887020367568	22.52783416024611	19.13808766196304
12	24.539057802622814	24.06820286981816	28.933162162601363	22.45957716495767
13	29.730406097144808	19.601540536587745	25.077189333246665	25.590864033020782
14	23.41284898634402	20.020589188807087	25.2553945055954	31.311167319253492
15	24.99343892266704	27.639076557819138	22.22511117268187	25.142373346831953
16	25.420214326166107	25.86027442004451	24.03982641351836	24.679684840271022
17	23.878746415782988	26.082049623682607	25.317714583792068	24.72148937674234
18	24.756021362705294	24.887324161096185	26.573460096868313	23.78319437933021
19	25.625252388191523	24.932743879103924	25.45576127591219	23.98624245679236
20	25.838700687308396	24.30751180384532	25.175849060283095	24.677938448563186
21	26.86643807790435	24.385654159286283	24.46146220972748	24.286445553081887
22	25.81877569592278	24.614215837626144	24.934185936916002	24.632822529535076
23	24.121546189209436	24.40739001350405	25.706199999674993	25.764863797611522
24	24.79737849412916	25.075665675124704	25.37377827864956	24.753177552096577
25	24.804691149980133	24.694432550093723	25.40416642629696	25.09670987362918
26	24.56063090395875	25.4465192542385	25.771363941427204	24.221485900375544
27	25.22029375751071	24.986451273742773	24.885780378194312	24.907474590552212
28	24.25811075098538	24.871561325150743	25.71308551013494	25.15724241372894
29	24.635525044627514	24.818097685706928	25.61915850831571	24.927218761349852
30	24.498534625019197	25.062584146324618	25.841869504843825	24.597011723812365
31	25.122385420839283	24.76861538111531	24.92128738493739	25.187711813108017
32	24.80948500214911	25.07769696841664	24.788115796717918	25.324702232716334
33	24.24616674642878	24.75480258673013	25.56683239311538	25.43219827372571
34	24.909099625185764	24.683626069780612	25.752573851730215	24.65470045330341
35	25.546763215391028	24.566948583091676	25.52295645800951	24.363331743507782
36	24.453358662206487	25.330796112592154	25.237925383284733	24.97791984191663
37	25.412779109854778	25.082815827512324	24.894474313483805	24.609930749149093
38	24.611474532050963	24.963213278483	25.419116744925624	25.006195444540413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	292.0
1	223.0
2	154.0
3	154.0
4	399.0
5	644.0
6	644.0
7	772.0
8	900.0
9	873.5
10	847.0
11	847.0
12	1216.5
13	1586.0
14	2086.0
15	2586.0
16	2586.0
17	3851.0
18	5116.0
19	5116.0
20	6352.5
21	7589.0
22	8549.0
23	9509.0
24	9509.0
25	11284.0
26	13059.0
27	13059.0
28	16819.0
29	20579.0
30	25205.0
31	29831.0
32	29831.0
33	37866.0
34	45901.0
35	45901.0
36	50790.0
37	55679.0
38	63166.5
39	70654.0
40	70654.0
41	74973.5
42	79293.0
43	88329.5
44	97366.0
45	97366.0
46	100355.5
47	103345.0
48	103345.0
49	109727.5
50	116110.0
51	115711.0
52	115312.0
53	115312.0
54	109448.5
55	103585.0
56	103585.0
57	100769.0
58	97953.0
59	86528.0
60	75103.0
61	75103.0
62	70359.0
63	65615.0
64	53881.0
65	42147.0
66	42147.0
67	35452.0
68	28757.0
69	28757.0
70	22957.5
71	17158.0
72	13330.5
73	9503.0
74	9503.0
75	7118.0
76	4733.0
77	4733.0
78	4495.5
79	4258.0
80	3309.0
81	2360.0
82	2360.0
83	2230.5
84	2101.0
85	2101.0
86	1343.0
87	585.0
88	489.0
89	393.0
90	393.0
91	230.5
92	68.0
93	51.5
94	35.0
95	35.0
96	25.0
97	15.0
98	15.0
99	18.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008775187021173388
2	0.0
3	0.0
4	6.50013853420251E-4
5	0.0
6	1.6250346335506275E-4
7	6.50013853420251E-4
8	0.0022750484869708786
9	0.003412572730456318
10	0.001056272511807908
11	0.004956355632329414
12	2.4375519503259415E-4
13	2.4375519503259415E-4
14	0.0
15	0.0
16	1.6250346335506275E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	2.4375519503259415E-4
22	2.4375519503259415E-4
23	8.125173167753137E-5
24	0.0
25	0.0
26	8.125173167753137E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1230743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.28249762896228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91499860319239	47.15928214238047
2	12.123221655243585	14.13143274754237
3	3.180716509036774	5.561403071890109
4	1.2965331843316532	3.02260768966722
5	0.6951827173349878	2.0258492537385995
6	0.395378712311955	1.3826195317718206
7	0.24512103796545337	1.0000386419821217
8	0.18253368947426252	0.8510815459191554
9	0.13393236421074414	0.7025321429598609
>10	0.6783087078796898	7.189403399315906
>50	0.07012779316970374	2.897250126666696
>100	0.0725935379284117	8.711306638837895
>500	0.00896907687545794	3.491087765976891
>1k	0.0023824110450435154	1.8741053013508906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3035	0.24659900564130774	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2138	0.17371620232656207	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1381	0.11220864144667084	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1322	0.10741478927769649	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1314	0.10676477542427623	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1275	0.10359595788885251	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1250	0.10156466459691421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6250346335506275E-4
2	0.0	0.0	0.0	0.0	1.6250346335506275E-4
3	0.0	0.0	0.0	0.0	1.6250346335506275E-4
4	0.0	0.0	0.0	0.0	1.6250346335506275E-4
5	0.0	0.0	0.0	0.0	1.6250346335506275E-4
6	0.0	0.0	0.0	0.0	1.6250346335506275E-4
7	0.0	0.0	0.0	0.0	1.6250346335506275E-4
8	0.0	0.0	0.0	0.0	1.6250346335506275E-4
9	0.0	0.0	0.0	0.0	1.6250346335506275E-4
10	0.0	0.0	0.0	0.0	1.6250346335506275E-4
11	0.0	0.0	0.0	0.0	1.6250346335506275E-4
12	0.0	0.0	0.0	0.0	3.250069267101255E-4
13	0.0	0.0	0.0	0.0	4.8751039006518824E-4
14	0.0	0.0	0.0	0.0	4.8751039006518824E-4
15	0.0	0.0	0.0	0.0	6.50013853420251E-4
16	0.0	0.0	0.0	1.6250346335506275E-4	6.50013853420251E-4
17	0.0	0.0	0.0	2.4375519503259412E-4	7.312655850977824E-4
18	0.0	0.0	0.0	3.250069267101255E-4	7.312655850977824E-4
19	0.0	0.0	0.0	3.250069267101255E-4	7.312655850977824E-4
20	0.0	0.0	0.0	4.062586583876569E-4	7.312655850977824E-4
21	0.0	0.0	0.0	5.687621217427196E-4	7.312655850977824E-4
22	0.0	0.0	0.0	6.50013853420251E-4	7.312655850977824E-4
23	0.0	0.0	0.0	0.0014625311701955649	7.312655850977824E-4
24	0.0	0.0	0.0	0.00235630021864841	7.312655850977824E-4
25	0.0	0.0	0.0	0.00235630021864841	7.312655850977824E-4
26	0.0	0.0	0.0	0.0027625588770360666	8.125173167753138E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGA	45	0.008853989	17.776604	14
GGTATAT	65	2.099659E-4	17.231731	1
TACCGTC	285	0.0	16.845783	7
GTATAGG	95	7.6889773E-7	16.843046	1
GCATTCG	470	0.0	15.998943	22
AGGGGCG	315	0.0	15.744992	32
ATACCGT	305	0.0	15.741143	6
TCTAGCG	295	0.0	15.727775	28
GCGAGAA	265	0.0	15.697077	20
CGTATTG	495	0.0	15.514127	27
ACCGTCG	290	0.0	15.451651	8
ATTATAC	125	5.1339157E-8	15.360858	3
AGGGACG	525	0.0	15.241425	8
CTAGCGG	305	0.0	15.212111	29
GCTATAT	75	6.242151E-4	14.934168	1
GTAGGAC	400	0.0	14.800826	3
TGTAGGA	435	0.0	14.712868	2
CCGATAA	120	5.4087104E-7	14.668677	9
CGGGGGC	535	0.0	14.653239	17
ATTCGTA	525	0.0	14.627605	24
>>END_MODULE