FastQCFastQC Report
Thu 2 Feb 2017
SRR4062723_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062723_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346173
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40130.29810432982982127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28440.21126556542138344No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17040.12658105607525927No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16470.12234683060795305No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16350.12145541472009913No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16120.11974686760171241No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16010.11892973637117964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15390.1143240876172676No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15360.11410123364530413No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14420.10711847585711494No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14090.10466708216551662No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13960.10370138162034152No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13680.101621411215349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA451.2383774E-524.88862812
TACCGTA552.2213535E-623.269897
GTATCGA453.5374533E-421.3331099
ACCGTAT555.8397145E-520.3611558
AGTATCG400.004487036719.997568
CGTTTGG550.001364905317.45436515
TATCACG652.0943551E-417.2376332
GTATTAG3550.016.2426821
CGGCATC3200.015.99983225
CGACCCG3350.015.7586875
ATCGTTT3250.015.75309629
CGTTTAT3250.015.75309631
TACCGTC3500.015.5409627
CACGTTT2800.015.42783828
ACCGTCG3450.015.3024828
GTCCTAA3950.015.0033491
TAGCGTA3200.014.9981717
ATAGCGT3200.014.9981716
GTACCGT756.2511116E-414.9315146
TAATACT1501.9790605E-914.929854
Produced by FastQC (version 0.11.5)