##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062723_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346173
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226499120098236	32.0	32.0	32.0	32.0	32.0
2	30.873046034944988	32.0	32.0	32.0	32.0	32.0
3	30.940284792519236	32.0	32.0	32.0	32.0	32.0
4	30.98976803130058	32.0	32.0	32.0	32.0	32.0
5	30.92140683255421	32.0	32.0	32.0	32.0	32.0
6	34.56685433447261	36.0	36.0	36.0	32.0	36.0
7	34.4919999138298	36.0	36.0	36.0	32.0	36.0
8	34.48462493305095	36.0	36.0	36.0	32.0	36.0
9	34.598355486256224	36.0	36.0	36.0	32.0	36.0
10	34.33656001123184	36.0	36.0	36.0	32.0	36.0
11	34.59426017309811	36.0	36.0	36.0	32.0	36.0
12	34.42895378231476	36.0	36.0	36.0	32.0	36.0
13	34.50212714116239	36.0	36.0	36.0	32.0	36.0
14	34.41187871098291	36.0	36.0	36.0	32.0	36.0
15	34.369532742076984	36.0	36.0	36.0	32.0	36.0
16	34.38759728504434	36.0	36.0	36.0	32.0	36.0
17	34.31488894815154	36.0	36.0	36.0	32.0	36.0
18	34.335335800079186	36.0	36.0	36.0	32.0	36.0
19	34.31722297208457	36.0	36.0	36.0	32.0	36.0
20	34.29343851050348	36.0	36.0	36.0	32.0	36.0
21	34.27252440808128	36.0	36.0	36.0	32.0	36.0
22	34.24215981155468	36.0	36.0	36.0	32.0	36.0
23	34.20073497239954	36.0	36.0	36.0	32.0	36.0
24	34.184161322504615	36.0	36.0	36.0	32.0	36.0
25	34.1657320418698	36.0	36.0	36.0	32.0	36.0
26	34.116229489077554	36.0	36.0	36.0	32.0	36.0
27	34.12186175179565	36.0	36.0	36.0	32.0	36.0
28	34.0974770701834	36.0	36.0	36.0	32.0	36.0
29	34.0660561458297	36.0	36.0	36.0	32.0	36.0
30	34.03247353794794	36.0	36.0	36.0	32.0	36.0
31	34.04430485531949	36.0	36.0	36.0	32.0	36.0
32	33.99632513800232	36.0	36.0	36.0	32.0	36.0
33	33.95810419611744	36.0	36.0	36.0	32.0	36.0
34	33.96011805317742	36.0	36.0	36.0	32.0	36.0
35	33.91457561546696	36.0	36.0	36.0	32.0	36.0
36	33.868799923932514	36.0	36.0	36.0	32.0	36.0
37	33.85744551406097	36.0	36.0	36.0	32.0	36.0
38	33.25261611991921	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	8.0
9	8.0
10	15.0
11	14.0
12	7.0
13	7.0
14	156.0
15	357.0
16	473.0
17	592.0
18	767.0
19	952.0
20	1295.0
21	1818.0
22	2696.0
23	3940.0
24	5698.0
25	8456.0
26	12446.0
27	17702.0
28	25177.0
29	34421.0
30	46708.0
31	63151.0
32	86303.0
33	129784.0
34	290422.0
35	612794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56850035885479	17.914477280587256	12.02561442261566	25.491407937942295
2	16.074652435829304	20.239996729682222	38.79146893012297	24.893881904365504
3	18.25642290544672	25.008413799969244	29.145551681534144	27.589611613049897
4	12.185866840003715	16.240319435416474	36.945157396229924	34.62865632834989
5	14.150982641096801	37.13422098876217	33.82512435297823	14.889672017162791
6	32.73345729021734	36.444512456478286	17.673513018815644	13.148517234488738
7	29.36259259589414	31.099666313071435	21.635422516587777	17.902318574446653
8	27.5969961988202	33.35507417017727	20.057407694919107	18.990521936083425
9	27.735352890051036	14.353164904878286	19.089862350223225	38.821619854847455
10	15.85812926446063	26.857456979322592	31.844846990131153	25.43956676608563
11	36.68892552062169	21.21205142738708	23.22953709557242	18.869485956418803
12	24.757358365258398	23.884479924167294	29.490736716833034	21.867424993741274
13	29.404405017961093	19.898288588484842	25.61470766327505	25.08259873027902
14	23.55649791386832	20.42982633399503	25.732672031571312	30.281003720565337
15	24.7475167777256	28.050826551902464	22.896022683366127	24.305633987005805
16	25.11424548942983	25.947451903967668	24.880739670357837	24.057562936244665
17	23.365478277086492	26.240319552293922	26.176866785597262	24.217335385022324
18	24.336013966532324	25.01918540943112	27.468231710714484	23.176568913322065
19	25.322145083224683	25.105809197989682	26.000200586157323	23.57184513262831
20	25.463587204019973	24.477240934595965	25.877975958320427	24.18119590306364
21	26.81833850396415	24.481599686196198	24.688796372237874	24.01126543760178
22	25.42440876966589	24.82301907285693	25.498923144699788	24.253649012777394
23	23.66215704192391	24.67507670819682	26.060950512997678	25.6018157368816
24	24.399543528264992	25.469399157191507	25.882333711745233	24.248723602798268
25	24.502596328659767	24.80914811146716	26.01507200549208	24.67318355438099
26	23.88191666970058	25.893586210534686	26.562953925894778	23.66154319386996
27	24.707640897362843	25.277909234023237	25.626711975115025	24.387737893498894
28	23.787806298622957	25.34426093106461	26.195255528679002	24.672677241633433
29	23.834914417871396	25.3501255927674	26.093281139837014	24.72167884952419
30	23.87122894860004	25.62509750317582	26.40311713010081	24.100556418123332
31	24.736956668704696	25.28767056121111	25.16843465166399	24.806938118420202
32	24.14411764050378	25.591445299528182	25.174601264275847	25.08983579569219
33	23.801762058900223	25.034044333039386	26.054742841775013	25.109450766285374
34	24.458205484438963	25.481987236160748	26.157177987949392	23.902629291450904
35	25.286995932165613	25.12272229136096	26.06705425636644	23.52322752010699
36	23.97832917396321	25.966951395921267	25.505792532748075	24.548926897367448
37	24.88068563413431	25.820193399226167	25.330900489144327	23.9682204774952
38	24.08579687577773	25.765510232433403	25.754069460344724	24.394623431444145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	754.0
1	568.5
2	383.0
3	383.0
4	794.0
5	1205.0
6	1205.0
7	1371.0
8	1537.0
9	1473.0
10	1409.0
11	1409.0
12	1797.5
13	2186.0
14	2792.0
15	3398.0
16	3398.0
17	5130.5
18	6863.0
19	6863.0
20	8335.5
21	9808.0
22	10517.0
23	11226.0
24	11226.0
25	13942.5
26	16659.0
27	16659.0
28	21705.0
29	26751.0
30	33024.0
31	39297.0
32	39297.0
33	48915.5
34	58534.0
35	58534.0
36	63099.0
37	67664.0
38	76002.5
39	84341.0
40	84341.0
41	87115.0
42	89889.0
43	99487.5
44	109086.0
45	109086.0
46	110086.5
47	111087.0
48	111087.0
49	115401.0
50	119715.0
51	118482.0
52	117249.0
53	117249.0
54	110544.5
55	103840.0
56	103840.0
57	99663.0
58	95486.0
59	84723.0
60	73960.0
61	73960.0
62	71159.5
63	68359.0
64	56636.5
65	44914.0
66	44914.0
67	37710.5
68	30507.0
69	30507.0
70	24723.5
71	18940.0
72	14858.5
73	10777.0
74	10777.0
75	8216.5
76	5656.0
77	5656.0
78	5550.0
79	5444.0
80	4271.5
81	3099.0
82	3099.0
83	3109.0
84	3119.0
85	3119.0
86	2081.5
87	1044.0
88	876.0
89	708.0
90	708.0
91	506.0
92	304.0
93	267.5
94	231.0
95	231.0
96	211.0
97	191.0
98	191.0
99	372.0
100	553.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12019257554563938
2	0.05482207710301722
3	0.012628391744597461
4	0.003565663551415754
5	3.7142328660580773E-4
6	4.4570794392696924E-4
7	7.428465732116155E-5
8	8.914158878539385E-4
9	2.2285397196348462E-4
10	0.0013371238317809078
11	0.0031199556074887847
12	0.0038628021807004
13	0.0154512087228016
14	0.010474136682283778
15	0.025331068146516086
16	0.012479822429955139
17	0.021542550623136848
18	0.007279896417473832
19	0.008765589563897062
20	0.006982757788189186
21	0.008097027648006608
22	0.009211297507824031
23	0.012108399143349331
24	0.014634077492268823
25	0.017382609813151803
26	0.020502565420640585
27	0.010399852024962616
28	0.0075027503894373165
29	0.011588406542101201
30	0.0046799334112331776
31	0.0075770350467584775
32	0.007874173676043124
33	0.009954144081035647
34	0.013594092289772564
35	0.018422595015648063
36	0.016045485981370892
37	0.01106841394085307
38	0.00817131230532777
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1346173.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.40613388674797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.72864773275589	42.57998835424926
2	12.326591175684975	13.166311573916756
3	3.648599067789176	5.845727109402377
4	1.4227106734945647	3.0392590684302445
5	0.7131174292916346	1.9042422452861283
6	0.43220809506835467	1.3849538035294107
7	0.2817382768757804	1.053258649509472
8	0.1991595769346849	0.8509074424481492
9	0.16038568572380557	0.7709021464755098
>10	0.8976594675410164	8.841626420459175
>50	0.08653906954589272	3.2348852210537173
>100	0.08825967559654495	10.262233913504575
>500	0.009496281664542978	3.414452612944202
>1k	0.00488779203322065	3.651251438791122
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4013	0.29810432982982127	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2844	0.21126556542138344	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1704	0.12658105607525927	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1647	0.12234683060795305	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1635	0.12145541472009913	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1612	0.11974686760171241	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1601	0.11892973637117964	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1539	0.1143240876172676	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1536	0.11410123364530413	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1442	0.10711847585711494	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1409	0.10466708216551662	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1396	0.10370138162034152	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1368	0.101621411215349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	7.428465732116154E-5
11	0.0	0.0	0.0	0.0	7.428465732116154E-5
12	0.0	0.0	0.0	0.0	7.428465732116154E-5
13	0.0	0.0	0.0	0.0	7.428465732116154E-5
14	0.0	0.0	0.0	0.0	7.428465732116154E-5
15	0.0	0.0	0.0	0.0	7.428465732116154E-5
16	0.0	0.0	0.0	7.428465732116154E-5	7.428465732116154E-5
17	0.0	0.0	0.0	7.428465732116154E-5	7.428465732116154E-5
18	7.428465732116154E-5	0.0	0.0	7.428465732116154E-5	7.428465732116154E-5
19	7.428465732116154E-5	0.0	0.0	7.428465732116154E-5	7.428465732116154E-5
20	7.428465732116154E-5	0.0	0.0	7.428465732116154E-5	7.428465732116154E-5
21	7.428465732116154E-5	0.0	0.0	3.7142328660580773E-4	7.428465732116154E-5
22	7.428465732116154E-5	0.0	0.0	8.914158878539385E-4	7.428465732116154E-5
23	7.428465732116154E-5	0.0	0.0	0.0017085471183867155	7.428465732116154E-5
24	7.428465732116154E-5	0.0	0.0	0.003194240264809946	7.428465732116154E-5
25	7.428465732116154E-5	0.0	0.0	0.003937086838021562	7.428465732116154E-5
26	7.428465732116154E-5	0.0	0.0	0.0046799334112331776	7.428465732116154E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	45	1.2383774E-5	24.888628	12
TACCGTA	55	2.2213535E-6	23.26989	7
GTATCGA	45	3.5374533E-4	21.333109	9
ACCGTAT	55	5.8397145E-5	20.361155	8
AGTATCG	40	0.0044870367	19.99756	8
CGTTTGG	55	0.0013649053	17.454365	15
TATCACG	65	2.0943551E-4	17.237633	2
GTATTAG	355	0.0	16.242682	1
CGGCATC	320	0.0	15.999832	25
CGACCCG	335	0.0	15.758687	5
ATCGTTT	325	0.0	15.753096	29
CGTTTAT	325	0.0	15.753096	31
TACCGTC	350	0.0	15.540962	7
CACGTTT	280	0.0	15.427838	28
ACCGTCG	345	0.0	15.302482	8
GTCCTAA	395	0.0	15.003349	1
TAGCGTA	320	0.0	14.998171	7
ATAGCGT	320	0.0	14.998171	6
GTACCGT	75	6.2511116E-4	14.931514	6
TAATACT	150	1.9790605E-9	14.92985	4
>>END_MODULE