FastQCFastQC Report
Thu 2 Feb 2017
SRR4062723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346173
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44640.3316067102816651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33580.24944787928446047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19220.1427751113712725No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18000.13371238317809078No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16900.125541070872763No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16160.12004400623099706No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15840.11766689719671988No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15810.1174440432247564No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15310.11372981035869834No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14800.10994129283531909No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14550.10808417640229004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.1042956588789108No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13920.10340424299105687No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13720.10191854984463364No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13480.10013571806892577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG400.004484776419.99930414
ACCGAAC507.1913056E-419.2021858
TAATCGG507.198499E-419.19933115
AATCGGA652.1012987E-417.2301716
GCCTTAT805.6201097E-516.0006311
AGAACCG3200.015.5017635
GAACCGC3000.015.4684266
TCTAGCG3050.015.21258528
AGTACTA859.42495E-515.0605375
ATATACT1603.0377123E-1015.0005914
AACCGCG3000.014.9350327
TCTAGAT3150.014.7301932
CGTCGTA2500.014.721127510
ATCGTTT2750.014.54494929
ATTCGTT1002.3270402E-514.39949932
GCATTCG4900.014.36684722
GTCCTAA3350.014.3289231
CTAGCGG3150.014.22172729
CATCGTT2850.014.03459928
ACCGCAG4450.014.02198429
Produced by FastQC (version 0.11.5)