FastQCFastQC Report
Thu 2 Feb 2017
SRR4062722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016213
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT97850.1950674742081327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT68800.13715526035278008No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA62710.1250146275686459No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG62600.12479533863494234No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG60840.12128671569568517No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT59830.11927324457713419No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC58730.11708035524009845No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC58620.11686106630639488No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG56740.11311321907582472No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT55760.111159554030102No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC55700.11103994188444549No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA55100.10984382042788056No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC53600.1068535167864682No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG52610.10487991638313604No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG51020.10171019452323894No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG50500.10067355592754933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87350.017.4178181
ATCGTTT11850.016.20177829
TTCGCGT600.002443387416.00005510
CATCGTT11950.015.93183828
TCGTTTA12550.015.17091330
TCTAGCG13000.015.13730628
CGTCGTA11950.014.72808510
CGTTTAT12950.014.70231231
CTAGCGG13300.014.6760129
CGGCATC12650.014.67110925
GCATCGT13050.014.46589627
GGCATCG13100.014.28884326
TACCGTC12350.014.2494987
TAGCGGC13750.014.19598730
ATACCGT12550.014.1490446
TTCGTTC16250.014.07934629
CAAGACG17250.014.0033324
CCGTCGT12500.013.9519099
CGCTTCG12500.013.95163132
TTTCGTT16400.013.95015528
Produced by FastQC (version 0.11.5)