##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062722_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5016213
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264009323368047	32.0	32.0	32.0	32.0	32.0
2	30.858975286735234	32.0	32.0	32.0	32.0	32.0
3	30.913434297945482	32.0	32.0	32.0	32.0	32.0
4	30.986930578904843	32.0	32.0	32.0	32.0	32.0
5	30.894100190721566	32.0	32.0	32.0	32.0	32.0
6	34.557406154802436	36.0	36.0	36.0	32.0	36.0
7	34.49170719026485	36.0	36.0	36.0	32.0	36.0
8	34.47031455801418	36.0	36.0	36.0	32.0	36.0
9	34.617237944242	36.0	36.0	36.0	32.0	36.0
10	34.32370595108302	36.0	36.0	36.0	32.0	36.0
11	34.608133665775355	36.0	36.0	36.0	32.0	36.0
12	34.41309031334993	36.0	36.0	36.0	32.0	36.0
13	34.503818916780446	36.0	36.0	36.0	32.0	36.0
14	34.40211111450012	36.0	36.0	36.0	32.0	36.0
15	34.3450617029221	36.0	36.0	36.0	32.0	36.0
16	34.36718416861485	36.0	36.0	36.0	32.0	36.0
17	34.28912328882366	36.0	36.0	36.0	32.0	36.0
18	34.31677622142441	36.0	36.0	36.0	32.0	36.0
19	34.304650540158484	36.0	36.0	36.0	32.0	36.0
20	34.28383603327849	36.0	36.0	36.0	32.0	36.0
21	34.27613321045179	36.0	36.0	36.0	32.0	36.0
22	34.25173512368793	36.0	36.0	36.0	32.0	36.0
23	34.19394312003896	36.0	36.0	36.0	32.0	36.0
24	34.180495126502805	36.0	36.0	36.0	32.0	36.0
25	34.163862658942115	36.0	36.0	36.0	32.0	36.0
26	34.114805332229714	36.0	36.0	36.0	32.0	36.0
27	34.126644343053215	36.0	36.0	36.0	32.0	36.0
28	34.1066280478919	36.0	36.0	36.0	32.0	36.0
29	34.07280332792886	36.0	36.0	36.0	32.0	36.0
30	34.04691606995158	36.0	36.0	36.0	32.0	36.0
31	34.06256432890709	36.0	36.0	36.0	32.0	36.0
32	34.02419215451975	36.0	36.0	36.0	32.0	36.0
33	33.98547190878856	36.0	36.0	36.0	32.0	36.0
34	33.996665014025524	36.0	36.0	36.0	32.0	36.0
35	33.96815067462247	36.0	36.0	36.0	32.0	36.0
36	33.919503418216095	36.0	36.0	36.0	32.0	36.0
37	33.92591642340547	36.0	36.0	36.0	32.0	36.0
38	33.33551824852732	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	5.0
7	10.0
8	8.0
9	14.0
10	47.0
11	47.0
12	22.0
13	26.0
14	360.0
15	888.0
16	1287.0
17	1656.0
18	2200.0
19	2961.0
20	4010.0
21	6083.0
22	9221.0
23	14208.0
24	21805.0
25	32827.0
26	48206.0
27	68770.0
28	95557.0
29	129301.0
30	173415.0
31	234660.0
32	321184.0
33	482565.0
34	1080676.0
35	2284193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.056133600544	17.57662597232609	11.541712547345167	25.825527879784737
2	16.786834393527297	19.522561666907347	37.87024677932674	25.820357160238615
3	18.353976859472496	23.96770406665901	28.818214951064835	28.86010412280366
4	12.164409811622729	15.696590804200355	36.46488044080892	35.674118943368
5	14.510571552398849	36.63296385031053	33.83273547017083	15.023729127119784
6	34.01547706668559	36.20757641846531	16.977725750748178	12.799220764100925
7	30.37466533498398	30.585820769066686	21.06799755193662	17.971516344012713
8	28.202449188547806	32.99776005384164	19.225240979611627	19.57454977799893
9	27.519610956578493	14.010881940336919	18.622100669410973	39.84740643367361
10	15.616769776110997	26.66636896402071	31.735155220365414	25.981706039502882
11	37.59866477967649	21.00930583335543	22.541890603877395	18.850138783090692
12	24.80713019215411	23.714802680500583	28.997555460660703	22.480511666684606
13	29.396711299186755	19.562091414653366	25.34214100516083	25.69905628099905
14	23.566002503322544	20.088864361626833	25.2924895937464	31.052643541304224
15	25.107661262583413	27.7877821391077	22.219466065540917	24.88509053276797
16	25.767061246071286	25.906724491171207	23.954788585136562	24.371425677620945
17	23.96001426865468	26.07429151722896	25.276794154716804	24.688900059399558
18	24.77716984773306	25.001475300283417	26.427193532283553	23.794161319699963
19	25.474912661287284	25.055718739339973	25.476387994219085	23.992980605153658
20	25.750744479428604	24.370300090451984	24.96034128129211	24.918614148827302
21	27.301323694622187	24.215307966590142	24.419140390977866	24.06422794780981
22	26.05180435632526	24.333106784057605	25.09658914082666	24.51849971879048
23	24.319051175483807	24.312691173872874	25.704753971611666	25.663503679031653
24	24.828032548802867	25.16526819744615	25.3411634849199	24.665535768831084
25	24.789935570006257	24.727985953567497	25.41008971229617	25.071988764130076
26	24.551096758525297	25.78382977297973	25.757370626838505	23.907702841656466
27	25.361754965402035	24.84474427289839	25.000951999867617	24.792548761831963
28	24.34477701624785	24.940977996884325	25.834432166662747	24.879812820205082
29	24.359436501103943	25.052824532434602	25.74822625607547	24.839512710385986
30	24.118226726689908	25.400389190154737	26.020278477696724	24.46110560545863
31	25.197083039434947	25.115462690090283	24.68379590845602	25.003658362018744
32	24.70993068002161	25.04622328728615	24.652333493491657	25.59151253920058
33	24.259300130408874	24.879026381429902	25.352792785464544	25.50888070269668
34	25.195391161141885	24.88637405843392	25.475777477380486	24.442457303043717
35	25.52973861695522	24.72996161022515	25.611167847496848	24.129131925322774
36	24.2195458229529	25.44655558978316	25.19116449288149	25.14273409438245
37	25.38653612196259	25.316077390562675	24.911487963200365	24.385898524274367
38	24.45472820094049	25.026251556801547	25.489698444524233	25.029321797733722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	199.5
2	300.0
3	300.0
4	771.5
5	1243.0
6	1243.0
7	1620.5
8	1998.0
9	2279.5
10	2561.0
11	2561.0
12	3568.0
13	4575.0
14	6731.0
15	8887.0
16	8887.0
17	13862.0
18	18837.0
19	18837.0
20	24018.0
21	29199.0
22	33907.5
23	38616.0
24	38616.0
25	47930.0
26	57244.0
27	57244.0
28	73350.0
29	89456.0
30	108860.0
31	128264.0
32	128264.0
33	161339.0
34	194414.0
35	194414.0
36	215857.0
37	237300.0
38	267564.0
39	297828.0
40	297828.0
41	313250.5
42	328673.0
43	366226.0
44	403779.0
45	403779.0
46	402857.5
47	401936.0
48	401936.0
49	424885.5
50	447835.0
51	452008.0
52	456181.0
53	456181.0
54	436088.0
55	415995.0
56	415995.0
57	397990.0
58	379985.0
59	338508.0
60	297031.0
61	297031.0
62	283285.0
63	269539.0
64	225401.0
65	181263.0
66	181263.0
67	152271.5
68	123280.0
69	123280.0
70	100411.5
71	77543.0
72	60422.5
73	43302.0
74	43302.0
75	33230.0
76	23158.0
77	23158.0
78	21830.5
79	20503.0
80	15997.0
81	11491.0
82	11491.0
83	11416.5
84	11342.0
85	11342.0
86	7733.5
87	4125.0
88	3486.5
89	2848.0
90	2848.0
91	2139.5
92	1431.0
93	1236.5
94	1042.0
95	1042.0
96	940.0
97	838.0
98	838.0
99	1555.0
100	2272.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11829641205427283
2	0.053127728029092866
3	0.011901408492821178
4	0.0032295279327253447
5	2.9903036414123565E-4
6	3.389010793600671E-4
7	5.980607282824713E-5
8	4.186425097977299E-4
9	2.3922429131298853E-4
10	4.3857786740714557E-4
11	0.002232760052254559
12	0.0026912732772711207
13	0.014692358558139376
14	0.009549036294910124
15	0.023483851263891704
16	0.011502701340632865
17	0.020413806192041684
18	0.005681576918683477
19	0.007894401613328621
20	0.0058211244219493865
21	0.006538797295888352
22	0.00757543589157797
23	0.009788260586223113
24	0.014532875697264051
25	0.016885247895175107
26	0.018619624007194274
27	0.009090523069893563
28	0.005721447633902308
29	0.010984382042788055
30	0.0031099157870688506
31	0.006120154786090623
32	0.006837827660029588
33	0.00897091092423707
34	0.012619081366760144
35	0.0162473164516738
36	0.015310354644031266
37	0.010186967738411426
38	0.006379314435013027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	5016213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.937956718697535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.28153702702917	26.471491570342398
2	15.705278181928223	12.544734405699081
3	6.978632856128632	8.361370108912377
4	3.655528705519553	5.839773888999853
5	2.1372454446357354	4.267860803254774
6	1.3624659471679104	3.264846361721475
7	0.9254828252617834	2.587332511344212
8	0.612448037210816	1.9567938562061464
9	0.44983919134033246	1.6169092338711686
>10	1.7385275440706678	11.083958918570389
>50	0.06617202669810125	1.8367455885554729
>100	0.060839975775564666	5.288760351286693
>500	0.012775667129963185	3.580422124183592
>1k	0.012424959794300852	9.376098680205754
>5k	8.016103093095854E-4	1.9229015968466312
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9785	0.1950674742081327	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6880	0.13715526035278008	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	6271	0.1250146275686459	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	6260	0.12479533863494234	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	6084	0.12128671569568517	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5983	0.11927324457713419	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5873	0.11708035524009845	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	5862	0.11686106630639488	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	5674	0.11311321907582472	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	5576	0.111159554030102	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	5570	0.11103994188444549	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	5510	0.10984382042788056	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5360	0.1068535167864682	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	5261	0.10487991638313604	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	5102	0.10171019452323894	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	5050	0.10067355592754933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.9806072828247125E-5
2	0.0	0.0	0.0	0.0	5.9806072828247125E-5
3	0.0	0.0	0.0	0.0	5.9806072828247125E-5
4	0.0	0.0	0.0	0.0	5.9806072828247125E-5
5	0.0	0.0	0.0	0.0	5.9806072828247125E-5
6	0.0	0.0	0.0	0.0	7.974143043766283E-5
7	0.0	0.0	0.0	0.0	7.974143043766283E-5
8	0.0	0.0	0.0	0.0	7.974143043766283E-5
9	0.0	0.0	0.0	0.0	7.974143043766283E-5
10	0.0	0.0	0.0	0.0	7.974143043766283E-5
11	0.0	0.0	0.0	1.9935357609415707E-5	7.974143043766283E-5
12	0.0	0.0	0.0	1.9935357609415707E-5	3.987071521883142E-4
13	0.0	0.0	0.0	1.9935357609415707E-5	4.585132250165613E-4
14	0.0	0.0	0.0	1.9935357609415707E-5	4.585132250165613E-4
15	0.0	0.0	0.0	7.974143043766283E-5	5.581900130636399E-4
16	0.0	0.0	0.0	9.967678804707855E-5	5.781253706730556E-4
17	0.0	0.0	0.0	1.1961214565649425E-4	5.980607282824713E-4
18	0.0	0.0	0.0	1.1961214565649425E-4	6.379314435013026E-4
19	0.0	0.0	0.0	1.3954750326590997E-4	6.379314435013026E-4
20	0.0	0.0	0.0	1.3954750326590997E-4	7.974143043766284E-4
21	0.0	0.0	0.0	2.591596489224042E-4	8.771557348142911E-4
22	0.0	0.0	0.0	4.3857786740714557E-4	8.771557348142911E-4
23	0.0	0.0	0.0	6.179960858918869E-4	8.771557348142911E-4
24	0.0	0.0	0.0	8.771557348142911E-4	8.970910924237069E-4
25	0.0	0.0	0.0	0.0010765093109084483	8.970910924237069E-4
26	0.0	0.0	0.0	0.0013356689598308525	9.170264500331226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8735	0.0	17.417818	1
ATCGTTT	1185	0.0	16.201778	29
TTCGCGT	60	0.0024433874	16.000055	10
CATCGTT	1195	0.0	15.931838	28
TCGTTTA	1255	0.0	15.170913	30
TCTAGCG	1300	0.0	15.137306	28
CGTCGTA	1195	0.0	14.728085	10
CGTTTAT	1295	0.0	14.702312	31
CTAGCGG	1330	0.0	14.67601	29
CGGCATC	1265	0.0	14.671109	25
GCATCGT	1305	0.0	14.465896	27
GGCATCG	1310	0.0	14.288843	26
TACCGTC	1235	0.0	14.249498	7
TAGCGGC	1375	0.0	14.195987	30
ATACCGT	1255	0.0	14.149044	6
TTCGTTC	1625	0.0	14.079346	29
CAAGACG	1725	0.0	14.003332	4
CCGTCGT	1250	0.0	13.951909	9
CGCTTCG	1250	0.0	13.951631	32
TTTCGTT	1640	0.0	13.950155	28
>>END_MODULE