FastQCFastQC Report
Thu 2 Feb 2017
SRR4062722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016213
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT102840.20501521765523115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT77570.15463856897623765No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG68160.13587939746577746No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA66450.1324704513145674No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC63950.12748661191221347No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC63530.12664932689261799No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA60440.12048930139130853No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT59430.11847583027275756No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG58870.11735945024663028No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC56510.11265470585080817No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG56400.1124354169171046No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT55590.11082065295074192No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC55490.11062129937464778No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC54170.1079898321702049No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA52000.10366385956896168No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG50610.1008928448612529No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG50440.10055394378189283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83300.017.0380631
TCTAGCG12550.016.19042828
ATCGTTT12900.015.87517629
CATCGTT13150.015.81670328
CTAGCGG13000.015.6299929
ACCGTCG13950.015.6010548
ATACCGT14350.015.50073056
TACCGTC13650.015.4749967
TCGTTTA13200.015.15076130
GCATCGT13450.015.10705327
TAGCGGC13750.014.66108830
CGTCGTA14850.014.65378310
CGTTTAT13900.014.38777331
CCGTCGT15300.014.3289419
CGGCATC14350.014.04808125
GTCGTAG15700.013.65632311
TAGGACG8700.013.6105514
CGAGCCG19050.013.52163615
AGCGGCG15600.013.43522731
TTTTCGG15800.013.36642329
Produced by FastQC (version 0.11.5)