FastQCFastQC Report
Thu 2 Feb 2017
SRR4062721_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062721_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962317
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT73660.24865671027104796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT49680.16770656212687568No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA42880.14475155764896194No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA41100.13874274765327277No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG41090.13870899029374642No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG40960.13827014461990395No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG40260.13590712945305988No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT40180.13563707057684915No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC39550.13351035692668947No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC37480.12652258350473633No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC36010.12156025165436381No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG34140.11524762542293751No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG34090.1150788386253058No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG32850.11089292604403918No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT31910.10771973424856286No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC31800.10734840329377308No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA31170.10522168964361342No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT30880.10424272621734945No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA30670.10353382166729623No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT30110.10164340953382099No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC29990.10123832121950486No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT29970.10117080650045218No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG29730.10036062987181993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC8800.015.9977467
ACCGTCG8650.015.5353848
AACCGCG5700.015.4364217
TCTAGCG8150.015.31227728
CGTCGTA8950.015.19518710
GTATCAA60500.015.1448271
CGCGGTC6150.015.08908810
ATAGCGT6950.014.9614156
TATACGG756.2567525E-414.9307275
ACTATAC1308.326424E-814.7688963
AGGCCCG11000.014.69057610
CGAGCCG11150.014.63693315
CCGTCGT9200.014.6081189
ATACCGT9750.014.6025796
TAAACGC8900.014.56122128
CATCGTT9150.014.51308628
GTTCAAA12500.014.4807831
AATAGCG7300.014.4632275
GTTATTC5100.014.4310463
CTATTCC11000.014.3979724
Produced by FastQC (version 0.11.5)