##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062721_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2962317
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24573534837764	32.0	32.0	32.0	32.0	32.0
2	30.85839564097968	32.0	32.0	32.0	32.0	32.0
3	30.909058686156815	32.0	32.0	32.0	32.0	32.0
4	30.97765634130311	32.0	32.0	32.0	32.0	32.0
5	30.888426188014314	32.0	32.0	32.0	32.0	32.0
6	34.53688717311483	36.0	36.0	36.0	32.0	36.0
7	34.477481309393966	36.0	36.0	36.0	32.0	36.0
8	34.45621552318675	36.0	36.0	36.0	32.0	36.0
9	34.59230426723406	36.0	36.0	36.0	32.0	36.0
10	34.305750532437955	36.0	36.0	36.0	32.0	36.0
11	34.57818930249531	36.0	36.0	36.0	32.0	36.0
12	34.3930737324871	36.0	36.0	36.0	32.0	36.0
13	34.488454476681596	36.0	36.0	36.0	32.0	36.0
14	34.381378157705605	36.0	36.0	36.0	32.0	36.0
15	34.32474917437938	36.0	36.0	36.0	32.0	36.0
16	34.34504950010414	36.0	36.0	36.0	32.0	36.0
17	34.26753416329178	36.0	36.0	36.0	32.0	36.0
18	34.292102431981455	36.0	36.0	36.0	32.0	36.0
19	34.290176574620475	36.0	36.0	36.0	32.0	36.0
20	34.263933603324695	36.0	36.0	36.0	32.0	36.0
21	34.25052788070959	36.0	36.0	36.0	32.0	36.0
22	34.2264166866679	36.0	36.0	36.0	32.0	36.0
23	34.17609391567479	36.0	36.0	36.0	32.0	36.0
24	34.15904982484994	36.0	36.0	36.0	32.0	36.0
25	34.13669435107722	36.0	36.0	36.0	32.0	36.0
26	34.08640128656049	36.0	36.0	36.0	32.0	36.0
27	34.090395457339646	36.0	36.0	36.0	32.0	36.0
28	34.07271976631805	36.0	36.0	36.0	32.0	36.0
29	34.04735786210591	36.0	36.0	36.0	32.0	36.0
30	34.0212239270814	36.0	36.0	36.0	32.0	36.0
31	34.03767152536342	36.0	36.0	36.0	32.0	36.0
32	33.99106138877102	36.0	36.0	36.0	32.0	36.0
33	33.94668632695286	36.0	36.0	36.0	32.0	36.0
34	33.962237329765856	36.0	36.0	36.0	32.0	36.0
35	33.927319392219	36.0	36.0	36.0	32.0	36.0
36	33.87933465594668	36.0	36.0	36.0	32.0	36.0
37	33.88648716528312	36.0	36.0	36.0	32.0	36.0
38	33.28280903090385	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	5.0
8	5.0
9	15.0
10	20.0
11	40.0
12	18.0
13	11.0
14	273.0
15	637.0
16	920.0
17	1171.0
18	1483.0
19	2023.0
20	2690.0
21	3800.0
22	5892.0
23	8792.0
24	13208.0
25	19881.0
26	28777.0
27	41105.0
28	57580.0
29	76765.0
30	103245.0
31	139219.0
32	190176.0
33	284410.0
34	637198.0
35	1342956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63745232677772	16.870879080023414	11.380431299861696	26.111237293337176
2	17.09339035667397	19.278444427406196	37.01821114826125	26.609954067658588
3	18.604345737265852	23.630955013560385	28.341791199475207	29.42290804969856
4	12.569626833928607	15.046586681610414	35.36435511204434	37.01943137241663
5	15.161478710870908	36.10277114819178	32.957894345966494	15.777855794970815
6	35.08662839920764	35.05813710120622	16.450146304840686	13.40508819474545
7	31.036121737181315	30.01506253890537	20.742722923550357	18.206092800362956
8	28.898905109735455	32.104144541448456	19.091545572942163	19.90540477587393
9	27.818106692484374	13.837825101238629	18.180520053957927	40.163548152319066
10	16.174600522228467	25.857837055941236	30.817555923512263	27.15000649831803
11	38.233422533276126	20.667740396771517	21.89089475295712	19.20794231699524
12	25.346775040231613	23.304322463231163	28.02516893562706	23.323733560910167
13	29.603768953736527	18.74108464108812	25.16088582837337	26.494260576801988
14	24.159241005987116	19.512657890241343	24.46386358526225	31.86423751850929
15	25.61008798557007	26.651881975485054	21.854071856570485	25.88395818237439
16	26.58503664100592	25.16639196321922	22.98751309089657	25.261058304878286
17	24.598972009666067	25.38720147644884	24.766477102178783	25.247349411706317
18	25.820228747479433	24.062271385026524	25.624119932575567	24.493379934918476
19	26.173371914291394	24.410828460917536	24.882152188172984	24.533647436618082
20	26.22512223455193	23.559880667073354	24.360992568530854	25.854004529843866
21	27.861661102515534	23.507984323374128	23.85972600001958	24.77062857409076
22	26.581019961912038	23.414405566878262	24.407319018895773	25.59725545231392
23	24.654560530232033	23.415711689034254	25.202057782111154	26.72766999862256
24	25.455239726108335	24.471952303605015	24.48093302240689	25.591874947879756
25	25.363241211612102	23.81945340106678	24.733389020077613	26.083916367243496
26	25.343210873622766	24.82307673607641	25.080392040343906	24.753320349956915
27	25.940634237708828	24.13242342112406	24.189613658676237	25.737328682490872
28	24.978090134956666	24.061623099006933	25.242966561967446	25.717320204068955
29	25.010643207607874	24.304595941300136	25.172730565975566	25.51203028511642
30	24.902530641787067	24.510121318082124	25.42595687751016	25.16139116262065
31	25.724748255648294	24.39887891559188	23.971619090573952	25.904753738185875
32	25.330963848018946	24.23927435412507	23.888071527487536	26.541690270368445
33	24.87752574145415	23.87781304238178	24.755144323983618	26.489516892180454
34	25.92461046421531	23.919325384218293	24.85935657341531	25.296707578151086
35	26.521855245525654	23.719498998760564	24.930102311780995	24.828543443932784
36	25.10621675240085	24.651841337966445	24.351220243440963	25.89072166619174
37	26.285976848821193	24.45431462227157	24.25667849084861	25.003030038058625
38	25.20586523490513	24.243484085111668	24.7219907823149	25.8286598976683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	238.5
2	325.0
3	325.0
4	823.0
5	1321.0
6	1321.0
7	1627.0
8	1933.0
9	2012.5
10	2092.0
11	2092.0
12	2768.5
13	3445.0
14	4588.0
15	5731.0
16	5731.0
17	8490.5
18	11250.0
19	11250.0
20	13567.0
21	15884.0
22	16676.0
23	17468.0
24	17468.0
25	20260.5
26	23053.0
27	23053.0
28	29815.0
29	36577.0
30	44872.0
31	53167.0
32	53167.0
33	71530.5
34	89894.0
35	89894.0
36	101380.0
37	112866.0
38	130885.5
39	148905.0
40	148905.0
41	159441.0
42	169977.0
43	196553.0
44	223129.0
45	223129.0
46	229829.0
47	236529.0
48	236529.0
49	256772.0
50	277015.0
51	281908.5
52	286802.0
53	286802.0
54	276354.0
55	265906.0
56	265906.0
57	260770.0
58	255634.0
59	229398.5
60	203163.0
61	203163.0
62	194009.0
63	184855.0
64	153170.0
65	121485.0
66	121485.0
67	102862.0
68	84239.0
69	84239.0
70	67228.5
71	50218.0
72	39296.0
73	28374.0
74	28374.0
75	21251.0
76	14128.0
77	14128.0
78	13898.0
79	13668.0
80	10667.5
81	7667.0
82	7667.0
83	7725.0
84	7783.0
85	7783.0
86	5136.0
87	2489.0
88	2155.0
89	1821.0
90	1821.0
91	1346.0
92	871.0
93	761.5
94	652.0
95	652.0
96	590.0
97	528.0
98	528.0
99	924.5
100	1321.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1226404871592068
2	0.05596970209467792
3	0.012827796620010618
4	0.003274463874055343
5	2.3630151668440618E-4
6	3.0381623573709363E-4
7	3.375735952634374E-5
8	3.713309547897811E-4
9	3.0381623573709363E-4
10	4.050883143161249E-4
11	0.0019579268525279367
12	0.0031731917954763112
13	0.014650694034433183
14	0.009823391622166028
15	0.0234613648708089
16	0.011308715441325152
17	0.020794533468227742
18	0.006278868871899935
19	0.007899222129164434
20	0.0062113541528472475
21	0.006751471905268747
22	0.007629163252953685
23	0.009890906341218714
24	0.014043061562958996
25	0.017250010717961648
26	0.01961302588480571
27	0.00921575915069184
28	0.006110082074268216
29	0.011274958081798807
30	0.003510765390739749
31	0.006245111512373592
32	0.007122802860058528
33	0.008979457634007435
34	0.013300399653379431
35	0.016473591448855743
36	0.015157054427328339
37	0.010397266734113871
38	0.006245111512373592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2962317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.375852111526854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.60850834278828	29.214871990067177
2	15.022513167608073	12.431385663328328
3	5.792228055608042	7.189751142752253
4	2.857230593131131	4.728814018796953
5	1.571970186675911	3.2520802983815886
6	0.9773760849829352	2.426386100975822
7	0.640336056305899	1.8546114977173844
8	0.4458422604644933	1.475768274723817
9	0.327463727440002	1.2194181682602176
>10	1.5476990769402135	11.020746945966788
>50	0.09049647068371805	2.5905268605758565
>100	0.08791666983272137	7.710382583493576
>500	0.017628439678646125	5.009733687140854
>1k	0.012708875117143085	9.625629774391806
>5k	8.199274269124571E-5	0.24989299342747193
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7366	0.24865671027104796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4968	0.16770656212687568	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4288	0.14475155764896194	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	4110	0.13874274765327277	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4109	0.13870899029374642	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	4096	0.13827014461990395	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	4026	0.13590712945305988	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4018	0.13563707057684915	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3955	0.13351035692668947	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3748	0.12652258350473633	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3601	0.12156025165436381	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3414	0.11524762542293751	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3409	0.1150788386253058	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3285	0.11089292604403918	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3191	0.10771973424856286	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3180	0.10734840329377308	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	3117	0.10522168964361342	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	3088	0.10424272621734945	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3067	0.10353382166729623	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	3011	0.10164340953382099	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2999	0.10123832121950486	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2997	0.10117080650045218	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2973	0.10036062987181993	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.375735952634374E-5	0.0	0.0	0.0
3	0.0	3.375735952634374E-5	0.0	0.0	0.0
4	0.0	3.375735952634374E-5	0.0	0.0	0.0
5	3.375735952634374E-5	1.3502943810537495E-4	0.0	0.0	0.0
6	6.751471905268747E-5	1.3502943810537495E-4	0.0	0.0	0.0
7	6.751471905268747E-5	1.3502943810537495E-4	0.0	0.0	0.0
8	6.751471905268747E-5	1.3502943810537495E-4	0.0	0.0	0.0
9	6.751471905268747E-5	1.3502943810537495E-4	0.0	3.375735952634374E-5	0.0
10	1.3502943810537495E-4	1.6878679763171868E-4	3.375735952634374E-5	3.375735952634374E-5	0.0
11	1.3502943810537495E-4	1.6878679763171868E-4	3.375735952634374E-5	3.375735952634374E-5	0.0
12	1.3502943810537495E-4	2.3630151668440616E-4	3.375735952634374E-5	3.375735952634374E-5	0.0
13	1.3502943810537495E-4	2.700588762107499E-4	3.375735952634374E-5	3.375735952634374E-5	0.0
14	1.3502943810537495E-4	3.0381623573709363E-4	3.375735952634374E-5	3.375735952634374E-5	0.0
15	2.0254415715806242E-4	3.3757359526343737E-4	3.375735952634374E-5	6.751471905268747E-5	0.0
16	2.0254415715806242E-4	3.3757359526343737E-4	3.375735952634374E-5	1.6878679763171868E-4	0.0
17	2.0254415715806242E-4	3.3757359526343737E-4	3.375735952634374E-5	1.6878679763171868E-4	0.0
18	2.0254415715806242E-4	3.713309547897811E-4	3.375735952634374E-5	1.6878679763171868E-4	0.0
19	2.0254415715806242E-4	3.713309547897811E-4	3.375735952634374E-5	2.0254415715806242E-4	0.0
20	2.0254415715806242E-4	3.713309547897811E-4	3.375735952634374E-5	2.3630151668440616E-4	0.0
21	2.0254415715806242E-4	3.713309547897811E-4	3.375735952634374E-5	4.0508831431612484E-4	0.0
22	2.700588762107499E-4	3.713309547897811E-4	3.375735952634374E-5	4.726030333688123E-4	0.0
23	2.700588762107499E-4	3.713309547897811E-4	3.375735952634374E-5	0.0012152649429483745	0.0
24	3.0381623573709363E-4	3.713309547897811E-4	3.375735952634374E-5	0.001924169493001593	0.0
25	4.388456738424686E-4	3.713309547897811E-4	3.375735952634374E-5	0.0022617430882650304	0.0
26	4.388456738424686E-4	3.713309547897811E-4	3.375735952634374E-5	0.0025993166835284677	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	880	0.0	15.997746	7
ACCGTCG	865	0.0	15.535384	8
AACCGCG	570	0.0	15.436421	7
TCTAGCG	815	0.0	15.312277	28
CGTCGTA	895	0.0	15.195187	10
GTATCAA	6050	0.0	15.144827	1
CGCGGTC	615	0.0	15.089088	10
ATAGCGT	695	0.0	14.961415	6
TATACGG	75	6.2567525E-4	14.930727	5
ACTATAC	130	8.326424E-8	14.768896	3
AGGCCCG	1100	0.0	14.690576	10
CGAGCCG	1115	0.0	14.636933	15
CCGTCGT	920	0.0	14.608118	9
ATACCGT	975	0.0	14.602579	6
TAAACGC	890	0.0	14.561221	28
CATCGTT	915	0.0	14.513086	28
GTTCAAA	1250	0.0	14.480783	1
AATAGCG	730	0.0	14.463227	5
GTTATTC	510	0.0	14.431046	3
CTATTCC	1100	0.0	14.397972	4
>>END_MODULE