FastQCFastQC Report
Thu 2 Feb 2017
SRR4062721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962317
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT78370.26455642660795586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT57300.1934296700859496No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG45000.15190811786854683No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC42990.14512288860375172No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA42530.1435700500655399No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA42280.14272611607738134No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC41620.14049813034864264No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG39320.13273393765758357No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT38880.13124861383842445No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC38520.13003334889547608No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA37700.1272652454143159No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG36500.12321436227115463No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG34800.1174756111516762No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG34730.1172393096349918No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC34570.1166991918825703No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG33390.11271582345846175No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG33210.11210819098698756No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT32300.10903627127009027No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT31790.10731464593424674No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT31560.10653822666514083No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC30650.10346630694824356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT30440.10275740239819034No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA30090.10157589481476831No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG29720.1003268725122936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT8350.015.52035229
CTAGCGG7950.015.4962529
CGTTTAT8350.015.13713431
TCTAGCG8200.015.02380528
AAGACGG12250.015.0221045
CAAGACG12500.014.4653894
TAGCGGC8800.014.36307630
CATCGTT8950.014.30111828
CGCAAGA11950.014.1926352
ACCGTCG9250.014.1853868
TACCGTC9200.014.0887847
GTATCAA60250.014.0753281
TCTAGAT7450.013.9598932
CGGCATC9300.013.76290325
GTATTAG7450.013.745591
ATACCGT9550.013.7400036
GCGCAAG12350.013.7334171
ACGAAAA7250.013.68222423
TAATATA9850.013.645984
GACGGAC12550.013.6432057
Produced by FastQC (version 0.11.5)