##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2962317
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.190359100663432	32.0	32.0	32.0	32.0	32.0
2	31.258791344748047	32.0	32.0	32.0	32.0	32.0
3	31.35293893259904	32.0	32.0	32.0	32.0	32.0
4	31.460990839265346	32.0	32.0	32.0	32.0	32.0
5	31.371189849026962	32.0	32.0	32.0	32.0	32.0
6	34.89625924571881	36.0	36.0	36.0	36.0	36.0
7	34.92256939416004	36.0	36.0	36.0	36.0	36.0
8	34.853173377460955	36.0	36.0	36.0	32.0	36.0
9	34.976879922034	36.0	36.0	36.0	36.0	36.0
10	34.81088485803511	36.0	36.0	36.0	32.0	36.0
11	35.00481447461565	36.0	36.0	36.0	36.0	36.0
12	34.89071358669582	36.0	36.0	36.0	32.0	36.0
13	34.954692897485316	36.0	36.0	36.0	36.0	36.0
14	34.890677128747534	36.0	36.0	36.0	32.0	36.0
15	34.859957931578556	36.0	36.0	36.0	32.0	36.0
16	34.87258757249815	36.0	36.0	36.0	32.0	36.0
17	34.836914482818685	36.0	36.0	36.0	32.0	36.0
18	34.8378043268158	36.0	36.0	36.0	32.0	36.0
19	34.832208706900715	36.0	36.0	36.0	32.0	36.0
20	34.82262769311995	36.0	36.0	36.0	32.0	36.0
21	34.81035216690179	36.0	36.0	36.0	32.0	36.0
22	34.797208738970205	36.0	36.0	36.0	32.0	36.0
23	34.73558130341891	36.0	36.0	36.0	32.0	36.0
24	34.708318184718244	36.0	36.0	36.0	32.0	36.0
25	34.69238032256507	36.0	36.0	36.0	32.0	36.0
26	34.62299544579463	36.0	36.0	36.0	32.0	36.0
27	34.61237875622359	36.0	36.0	36.0	32.0	36.0
28	34.58223309659297	36.0	36.0	36.0	32.0	36.0
29	34.54616538338065	36.0	36.0	36.0	32.0	36.0
30	34.52629445126906	36.0	36.0	36.0	32.0	36.0
31	34.52545929419438	36.0	36.0	36.0	32.0	36.0
32	34.49270486581956	36.0	36.0	36.0	32.0	36.0
33	34.455048193694324	36.0	36.0	36.0	32.0	36.0
34	34.457485137478535	36.0	36.0	36.0	32.0	36.0
35	34.418680715129405	36.0	36.0	36.0	32.0	36.0
36	34.396376214969564	36.0	36.0	36.0	32.0	36.0
37	34.3875007300029	36.0	36.0	36.0	32.0	36.0
38	33.93916822541274	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	20.0
21	67.0
22	260.0
23	742.0
24	2131.0
25	5181.0
26	10656.0
27	20237.0
28	35500.0
29	54819.0
30	82975.0
31	119806.0
32	173499.0
33	280499.0
34	684109.0
35	1491811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.064494565921954	18.041368674646733	12.033565965170045	26.860570794261267
2	16.03427317197991	20.349003837199056	36.266847876172605	27.34987511464843
3	17.593694395299355	24.3809153443065	28.586879797131772	29.438510463262375
4	12.029143433140444	15.888896315515504	35.44533091360335	36.6366293377407
5	14.624238138077821	36.6053238767653	32.681298241409166	16.08913974374771
6	35.102433366325535	35.47633000665695	16.089944489379256	13.331292137638254
7	30.790952441715934	30.22652653240622	20.68583824336614	18.29668278251171
8	28.782252943624933	32.3005257154277	18.915627957001586	20.001593383945778
9	27.8148878960099	13.684940778913004	17.958378977822264	40.541792347254834
10	15.989256956360437	26.052319675764245	30.834364911728734	27.12405845614658
11	38.374928388671684	20.520017244109134	21.84131165959021	19.263742707628964
12	25.214722704484917	23.359784546446708	27.957110483747645	23.468382265320734
13	29.601294664000733	18.744778151397238	25.099609663124728	26.554317521477305
14	24.08868601459129	19.453495170670514	24.423255317798642	32.034563496939555
15	25.619979225720947	26.673715203335767	21.747908815970742	25.958396754972547
16	26.573320816104424	25.2269085313962	22.870948652693144	25.328821999806234
17	24.610566661164217	25.45693117920871	24.566074461308496	25.366427698318578
18	25.840549812866076	24.032640666073213	25.511786888439016	24.615022632621695
19	26.20320512625759	24.40363404726773	24.794004152830368	24.599156673644313
20	26.296206651752662	23.45461339890363	24.370180503977124	25.878999445366585
21	27.82493127831615	23.54030349953128	23.828321874374435	24.80644334777814
22	26.473164719595804	23.51027727319834	24.42321253696014	25.593345470245715
23	24.691651422385146	23.290077719673782	25.22049493081926	26.797775927121815
24	25.467834806335716	24.47877792957337	24.440227024994286	25.613160239096626
25	25.418355662739106	23.819469387424416	24.73611827518506	26.02605667465142
26	25.45440796998025	24.75961376166486	24.986193235292927	24.79978503306197
27	25.984348721978833	24.042218346873423	24.14926304233785	25.824169888809895
28	24.97599007803689	24.0046895723854	25.298035287918207	25.721285061659504
29	25.182922691933374	24.153120682222735	25.195682973834334	25.46827365200956
30	24.947465109237125	24.328321378164457	25.459631767970812	25.264581744627602
31	25.725572246319352	24.225834034642478	24.081723866824518	25.96686985221365
32	25.488325523568207	24.129659317351923	23.912869554473744	26.469145604606126
33	24.89561380500466	23.77827896204221	24.796164623840056	26.52994260911307
34	25.808142747720787	23.852376366202538	24.96157568551914	25.37790520055754
35	26.575987647507006	23.663368910214537	24.887545796077866	24.87309764620059
36	25.240175173690055	24.55955929091991	24.360222082916852	25.840043452473182
37	26.379756116580367	24.317789081992238	24.26941478579099	25.03304001563641
38	25.316745411360568	24.057899292310474	24.737266382114534	25.888088914214418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	121.0
2	162.0
3	162.0
4	575.0
5	988.0
6	988.0
7	1378.5
8	1769.0
9	1900.5
10	2032.0
11	2032.0
12	2716.0
13	3400.0
14	4519.5
15	5639.0
16	5639.0
17	8704.0
18	11769.0
19	11769.0
20	13851.0
21	15933.0
22	16673.5
23	17414.0
24	17414.0
25	20132.0
26	22850.0
27	22850.0
28	29483.5
29	36117.0
30	44358.5
31	52600.0
32	52600.0
33	71671.5
34	90743.0
35	90743.0
36	101093.5
37	111444.0
38	129914.5
39	148385.0
40	148385.0
41	159462.5
42	170540.0
43	197914.5
44	225289.0
45	225289.0
46	230815.0
47	236341.0
48	236341.0
49	257143.0
50	277945.0
51	282325.5
52	286706.0
53	286706.0
54	276953.0
55	267200.0
56	267200.0
57	262122.0
58	257044.0
59	230404.5
60	203765.0
61	203765.0
62	194503.0
63	185241.0
64	153630.0
65	122019.0
66	122019.0
67	103351.0
68	84683.0
69	84683.0
70	68200.5
71	51718.0
72	40076.0
73	28434.0
74	28434.0
75	20874.5
76	13315.0
77	13315.0
78	13470.5
79	13626.0
80	10460.0
81	7294.0
82	7294.0
83	6920.5
84	6547.0
85	6547.0
86	4143.0
87	1739.0
88	1429.5
89	1120.0
90	1120.0
91	675.0
92	230.0
93	167.5
94	105.0
95	105.0
96	80.0
97	55.0
98	55.0
99	45.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009080729712586466
2	0.0
3	0.0
4	1.0127207857903122E-4
5	6.751471905268747E-5
6	3.375735952634374E-5
7	5.06360392895156E-4
8	0.002295500447791374
9	0.003780824266950499
10	9.452060667376247E-4
11	0.0049285744908461855
12	2.700588762107499E-4
13	1.3502943810537495E-4
14	3.375735952634374E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0254415715806245E-4
22	1.3502943810537495E-4
23	1.3502943810537495E-4
24	0.0
25	1.6878679763171868E-4
26	3.375735952634374E-5
27	1.0127207857903122E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.375735952634374E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2962317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.809878436526105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74470366161081	26.291637138256004
2	16.335489227900705	12.679567022720159
3	6.394312210337979	7.444874387052344
4	3.1384016251237634	4.8720394222419685
5	1.7266605879868315	3.35057437604548
6	1.106422053986279	2.5764063248819413
7	0.7206373606980238	1.9577493855856518
8	0.4932346089625762	1.5313900171620056
9	0.3666672406839575	1.2807279933840763
>10	1.7468720389407655	11.691058023269122
>50	0.09689271299795328	2.613977076775611
>100	0.09695502621779853	8.118671996660265
>500	0.01921429813758342	5.3089420344592435
>1k	0.013362670977493801	9.822522348391828
>5k	1.7467543761429805E-4	0.459862453114294
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7837	0.26455642660795586	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5730	0.1934296700859496	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4500	0.15190811786854683	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4299	0.14512288860375172	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4253	0.1435700500655399	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	4228	0.14272611607738134	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4162	0.14049813034864264	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3932	0.13273393765758357	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3888	0.13124861383842445	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3852	0.13003334889547608	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3770	0.1272652454143159	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3650	0.12321436227115463	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3480	0.1174756111516762	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3473	0.1172393096349918	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3457	0.1166991918825703	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	3339	0.11271582345846175	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3321	0.11210819098698756	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	3230	0.10903627127009027	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	3179	0.10731464593424674	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3156	0.10653822666514083	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3065	0.10346630694824356	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3044	0.10275740239819034	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	3009	0.10157589481476831	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	2972	0.1003268725122936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	6.751471905268747E-5	0.0	0.0	0.0	0.0
11	6.751471905268747E-5	0.0	0.0	0.0	0.0
12	6.751471905268747E-5	0.0	0.0	0.0	0.0
13	6.751471905268747E-5	1.0127207857903121E-4	0.0	0.0	0.0
14	6.751471905268747E-5	1.0127207857903121E-4	0.0	0.0	0.0
15	6.751471905268747E-5	1.6878679763171868E-4	0.0	0.0	0.0
16	6.751471905268747E-5	1.6878679763171868E-4	0.0	1.3502943810537495E-4	0.0
17	6.751471905268747E-5	1.6878679763171868E-4	0.0	1.6878679763171868E-4	0.0
18	6.751471905268747E-5	2.0254415715806242E-4	0.0	1.6878679763171868E-4	0.0
19	6.751471905268747E-5	2.0254415715806242E-4	0.0	1.6878679763171868E-4	0.0
20	6.751471905268747E-5	2.700588762107499E-4	0.0	2.0254415715806242E-4	0.0
21	6.751471905268747E-5	2.700588762107499E-4	0.0	3.713309547897811E-4	0.0
22	6.751471905268747E-5	2.700588762107499E-4	0.0	4.726030333688123E-4	0.0
23	6.751471905268747E-5	3.0381623573709363E-4	0.0	0.0011139928643693433	0.0
24	6.751471905268747E-5	3.3757359526343737E-4	0.0	0.0018228974144225618	0.0
25	6.751471905268747E-5	3.3757359526343737E-4	0.0	0.0021267136501596554	0.0
26	6.751471905268747E-5	3.3757359526343737E-4	0.0	0.0023967725263704053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	835	0.0	15.520352	29
CTAGCGG	795	0.0	15.49625	29
CGTTTAT	835	0.0	15.137134	31
TCTAGCG	820	0.0	15.023805	28
AAGACGG	1225	0.0	15.022104	5
CAAGACG	1250	0.0	14.465389	4
TAGCGGC	880	0.0	14.363076	30
CATCGTT	895	0.0	14.301118	28
CGCAAGA	1195	0.0	14.192635	2
ACCGTCG	925	0.0	14.185386	8
TACCGTC	920	0.0	14.088784	7
GTATCAA	6025	0.0	14.075328	1
TCTAGAT	745	0.0	13.959893	2
CGGCATC	930	0.0	13.762903	25
GTATTAG	745	0.0	13.74559	1
ATACCGT	955	0.0	13.740003	6
GCGCAAG	1235	0.0	13.733417	1
ACGAAAA	725	0.0	13.682224	23
TAATATA	985	0.0	13.64598	4
GACGGAC	1255	0.0	13.643205	7
>>END_MODULE