FastQCFastQC Report
Thu 2 Feb 2017
SRR4062720_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062720_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833563
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30530.36625905900333866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22450.2693257738167361No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14300.1715527200703486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12310.14767929958503437No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11410.1368822752449425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.11072948295449773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1203.6379788E-1219.99958629
CGCCAAT902.0367224E-819.55515326
GCTATCG852.3930443E-718.8242713
ACCGTGT550.001364956517.4531388
TAGCGGC1404.0017767E-1117.14250430
GAATATA957.630115E-716.8538911
CTATCGG957.682993E-716.84276814
TGCGCTA1051.1873817E-716.76256610
TCTAGCG1456.91216E-1116.55138228
CCTTATA600.002438554516.002552
CGCTATC1001.3127683E-616.0006312
GCGCTAT1102.0163134E-716.0006311
GTACCGT600.002443442615.9977496
ATGGTCG1001.3160607E-615.996795
AATAGCG1001.3160607E-615.996795
TCCAATA1551.8735591E-1015.486342
ATCGTTT1357.899871E-915.40708929
CATCGTT1357.899871E-915.40708928
TTCCGAA1153.342302E-715.30403120
GTCTAGT756.208755E-414.9437851
Produced by FastQC (version 0.11.5)