FastQCFastQC Report
Thu 2 Feb 2017
SRR4062720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833563
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33900.40668791681012717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25180.30207674764834813No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14930.1791106371084129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14520.1741919926868155No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13930.16711394339719973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.11000968133182494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA350.002067260222.85487415
CTAGCGG854.311005E-1022.58599329
TCTAGCG908.785719E-1021.33121528
AAGGCGT751.5029273E-619.2061586
CGGTAAT507.1897666E-419.2015481
CTATCGG1055.977199E-918.28389714
GTTTTCG1950.018.0494928
TAATACG450.00884112317.7802774
TCGACGA450.008853354517.77601214
TAGCGGC1101.0631993E-817.45281230
TTTTCGG2050.017.16902529
CGGCTCG1700.016.93949528
TATCGGT1151.8408173E-816.69399515
TTGTGCG1456.91216E-1116.5560387
TTCGGAA2150.016.37046631
CGCCCCT1203.093919E-816.005135
GTAATAC908.563691E-616.002253
CGTCGTA1304.783942E-916.0022510
CGCGGTT2300.016.00224910
CGAAAAA703.6976705E-415.99841124
Produced by FastQC (version 0.11.5)