##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833563
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129846214383317	32.0	32.0	32.0	32.0	32.0
2	31.25028582122767	32.0	32.0	32.0	32.0	32.0
3	31.357297528801062	32.0	32.0	32.0	32.0	32.0
4	31.462489337938464	32.0	32.0	32.0	32.0	32.0
5	31.37674536897631	32.0	32.0	32.0	32.0	32.0
6	34.88864908831126	36.0	36.0	36.0	36.0	36.0
7	34.91862642655684	36.0	36.0	36.0	36.0	36.0
8	34.861071088807925	36.0	36.0	36.0	32.0	36.0
9	34.98704117145315	36.0	36.0	36.0	36.0	36.0
10	34.82946100054825	36.0	36.0	36.0	32.0	36.0
11	35.01042152782693	36.0	36.0	36.0	36.0	36.0
12	34.899708840243626	36.0	36.0	36.0	32.0	36.0
13	34.95638242100477	36.0	36.0	36.0	36.0	36.0
14	34.896792444002436	36.0	36.0	36.0	32.0	36.0
15	34.870166981979764	36.0	36.0	36.0	32.0	36.0
16	34.88169100595876	36.0	36.0	36.0	32.0	36.0
17	34.853540764165395	36.0	36.0	36.0	32.0	36.0
18	34.846372739672944	36.0	36.0	36.0	32.0	36.0
19	34.838760837513185	36.0	36.0	36.0	32.0	36.0
20	34.82322511915716	36.0	36.0	36.0	32.0	36.0
21	34.80569794964508	36.0	36.0	36.0	32.0	36.0
22	34.79247999251406	36.0	36.0	36.0	32.0	36.0
23	34.736541809077416	36.0	36.0	36.0	32.0	36.0
24	34.71240206199172	36.0	36.0	36.0	32.0	36.0
25	34.7028191030552	36.0	36.0	36.0	32.0	36.0
26	34.638972699124125	36.0	36.0	36.0	32.0	36.0
27	34.6200971012389	36.0	36.0	36.0	32.0	36.0
28	34.57354872997002	36.0	36.0	36.0	32.0	36.0
29	34.54650218399809	36.0	36.0	36.0	32.0	36.0
30	34.522736733756176	36.0	36.0	36.0	32.0	36.0
31	34.508356297004546	36.0	36.0	36.0	32.0	36.0
32	34.47703772840205	36.0	36.0	36.0	32.0	36.0
33	34.43015464937863	36.0	36.0	36.0	32.0	36.0
34	34.42212526227772	36.0	36.0	36.0	32.0	36.0
35	34.39049118063062	36.0	36.0	36.0	32.0	36.0
36	34.350109109929306	36.0	36.0	36.0	32.0	36.0
37	34.319909832850065	36.0	36.0	36.0	32.0	36.0
38	33.90799975526745	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	18.0
22	55.0
23	186.0
24	582.0
25	1340.0
26	2979.0
27	5555.0
28	9983.0
29	15846.0
30	24128.0
31	34690.0
32	49267.0
33	79077.0
34	188733.0
35	421121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01834634530867	18.44065063476123	12.294366996495345	26.246636023434753
2	15.221404980787295	21.11118175830741	37.66481957572493	26.002593685180365
3	17.39388624495089	25.91717722595653	29.075666746244732	27.613269782847848
4	11.644872594654254	16.788713469936177	36.86909160708287	34.6973223283267
5	13.390605617818471	37.92711280024761	33.77169304742779	14.91058853450613
6	32.87409769434437	36.80159412831006	17.213538573754228	13.110769603591347
7	29.145902264749424	31.21162534580677	21.756854352066682	17.885618037377125
8	27.915569941286698	33.40503537914106	19.66499588503039	19.014398794541847
9	27.769486401209313	13.97682147013305	18.43772869602774	39.8159634326299
10	15.072580258169776	27.628844954172465	32.68307020490427	24.61550458275349
11	36.89029657356752	21.505782704674154	22.376907572703715	19.22701314905461
12	24.168717106486508	24.32971312237497	29.57264075454586	21.928929016592665
13	29.779188590650723	20.14019353089512	25.219719708911875	24.860898169542278
14	23.450417065056868	19.60523679673882	26.09640783000205	30.84793830820226
15	25.052935411000725	27.22049803074273	23.296019616993558	24.43054694126299
16	25.018864800860886	26.14859344764583	24.490170509007715	24.342371242485573
17	23.548669986551708	25.864751674438523	26.035824526760425	24.550753812249344
18	24.240159412066035	25.170982877119062	26.925019464635547	23.663838246179353
19	24.89253961608181	25.647131650517117	26.176305810118734	23.284022923282343
20	25.382364620310643	24.49041044288194	26.220933510724443	23.90629142608297
21	26.314387754734796	24.629452122994902	25.23300578360604	23.823154338664267
22	25.13391309355142	24.799565239819906	25.75582169554071	24.310699971087967
23	23.960665193470913	24.70925977911661	25.98462981757806	25.345445209834423
24	24.116473499903428	25.63585475842858	25.89594307808768	24.351728663580317
25	24.49544905424065	24.78948801710249	25.8971427474588	24.817920181198062
26	24.231761726468186	25.15754658016251	26.431355518419124	24.179336174950183
27	24.904266039637218	25.2430538893421	25.626949469744233	24.225730601276453
28	24.003944512892247	24.940766324800883	26.358415620654945	24.69687354165192
29	24.111434888544718	25.40911724728665	26.318226696722384	24.16122116744625
30	24.296423905571622	25.310024557232026	26.46950500442078	23.924046532775566
31	24.303262020987017	25.36772865398296	25.62829684139051	24.70071248363951
32	23.958716977600975	25.486375954786862	25.62469783327715	24.930209234335017
33	23.95907687841231	25.054015113434737	25.977040727575478	25.009867280577474
34	24.389878149581975	25.207812726812488	26.38840735493298	24.013901768672554
35	25.09708324385799	25.06217286515836	25.955806579706632	23.884937311277014
36	24.267151972916263	25.67484401299002	25.82468271744307	24.233321296650644
37	24.928049829467	25.72559002738845	25.537481869996633	23.808878273147922
38	24.111914756293164	25.44642696472852	25.890424598980523	24.551233679997793
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161.0
1	147.0
2	133.0
3	133.0
4	437.0
5	741.0
6	741.0
7	915.5
8	1090.0
9	1093.5
10	1097.0
11	1097.0
12	1382.0
13	1667.0
14	2026.5
15	2386.0
16	2386.0
17	3737.5
18	5089.0
19	5089.0
20	5986.5
21	6884.0
22	7155.5
23	7427.0
24	7427.0
25	8406.5
26	9386.0
27	9386.0
28	12214.0
29	15042.0
30	18010.5
31	20979.0
32	20979.0
33	25761.5
34	30544.0
35	30544.0
36	34078.0
37	37612.0
38	42621.5
39	47631.0
40	47631.0
41	51916.5
42	56202.0
43	62618.5
44	69035.0
45	69035.0
46	73752.0
47	78469.0
48	78469.0
49	79865.0
50	81261.0
51	78581.0
52	75901.0
53	75901.0
54	71948.0
55	67995.0
56	67995.0
57	64798.0
58	61601.0
59	54781.5
60	47962.0
61	47962.0
62	44200.0
63	40438.0
64	33304.5
65	26171.0
66	26171.0
67	21892.5
68	17614.0
69	17614.0
70	13848.0
71	10082.0
72	7845.5
73	5609.0
74	5609.0
75	4080.0
76	2551.0
77	2551.0
78	2365.0
79	2179.0
80	1645.0
81	1111.0
82	1111.0
83	1036.0
84	961.0
85	961.0
86	630.0
87	299.0
88	241.5
89	184.0
90	184.0
91	109.5
92	35.0
93	21.5
94	8.0
95	8.0
96	11.0
97	14.0
98	14.0
99	13.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01199669371121319
2	0.0
3	0.0
4	3.5990081133639566E-4
5	1.199669371121319E-4
6	4.798677484485276E-4
7	5.998346855606595E-4
8	0.00251930567935477
9	0.0039589089247003524
10	3.5990081133639566E-4
11	0.0051585782958216714
12	2.399338742242638E-4
13	1.199669371121319E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.199669371121319E-4
24	0.0
25	0.0
26	0.0
27	3.5990081133639566E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	833563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.50873780666954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12000307930349	48.665715246677635
2	13.593901548783426	16.72287452267597
3	3.7691730963763623	6.955112391988992
4	1.384916437959681	3.4073784826643494
5	0.657406143034505	2.0218111042201623
6	0.3372202950352188	1.2445196826245408
7	0.21604569463209125	0.9302088589769565
8	0.1421332814265584	0.6993950992694201
9	0.11140937297253535	0.6167384920235818
>10	0.5405469792198401	6.351031292417564
>50	0.06362340589398945	2.768943039159946
>100	0.05930074365630249	7.120140352496995
>500	0.0033381213182874328	1.2586338453617159
>1k	9.818003877315978E-4	1.2374975894421016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3390	0.40668791681012717	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2518	0.30207674764834813	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1493	0.1791106371084129	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1452	0.1741919926868155	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1393	0.16711394339719973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	917	0.11000968133182494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.199669371121319E-4	0.0
10	0.0	0.0	0.0	1.199669371121319E-4	0.0
11	0.0	0.0	0.0	1.199669371121319E-4	0.0
12	0.0	0.0	0.0	2.399338742242638E-4	0.0
13	0.0	0.0	0.0	2.399338742242638E-4	0.0
14	0.0	0.0	0.0	2.399338742242638E-4	0.0
15	0.0	0.0	0.0	2.399338742242638E-4	0.0
16	0.0	0.0	0.0	2.399338742242638E-4	0.0
17	0.0	0.0	0.0	2.399338742242638E-4	0.0
18	0.0	0.0	0.0	2.399338742242638E-4	0.0
19	0.0	0.0	0.0	3.599008113363957E-4	0.0
20	0.0	0.0	0.0	3.599008113363957E-4	0.0
21	0.0	0.0	0.0	4.798677484485276E-4	0.0
22	0.0	0.0	0.0	0.001079702434009187	0.0
23	0.0	0.0	0.0	0.0017995040566819785	0.0
24	0.0	0.0	0.0	0.0031191403649154293	0.0
25	0.0	0.0	0.0	0.0038389419875882206	0.0
26	0.0	0.0	0.0	0.0051585782958216714	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	35	0.0020672602	22.854874	15
CTAGCGG	85	4.311005E-10	22.585993	29
TCTAGCG	90	8.785719E-10	21.331215	28
AAGGCGT	75	1.5029273E-6	19.206158	6
CGGTAAT	50	7.1897666E-4	19.201548	1
CTATCGG	105	5.977199E-9	18.283897	14
GTTTTCG	195	0.0	18.04949	28
TAATACG	45	0.008841123	17.780277	4
TCGACGA	45	0.0088533545	17.776012	14
TAGCGGC	110	1.0631993E-8	17.452812	30
TTTTCGG	205	0.0	17.169025	29
CGGCTCG	170	0.0	16.939495	28
TATCGGT	115	1.8408173E-8	16.693995	15
TTGTGCG	145	6.91216E-11	16.556038	7
TTCGGAA	215	0.0	16.370466	31
CGCCCCT	120	3.093919E-8	16.00513	5
GTAATAC	90	8.563691E-6	16.00225	3
CGTCGTA	130	4.783942E-9	16.00225	10
CGCGGTT	230	0.0	16.002249	10
CGAAAAA	70	3.6976705E-4	15.998411	24
>>END_MODULE