FastQCFastQC Report
Thu 2 Feb 2017
SRR4062719_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062719_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910107
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38820.4265432526065617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27910.30666723802805607No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA16030.17613313599389963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14760.16217873283031556No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA14630.1607503293568778No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13450.14778482090567374No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG13280.14591690867117824No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC12200.13405017212261855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.13009459327309866No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11220.12328220747670328No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10680.11734883920242345No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10360.11383276911396131No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10190.11196485687946583No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10170.11174510249893693No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9620.10570185703439267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC400.004486161319.9973893
TCTAGCG1600.018.99960928
CTAGCGG1650.018.42386229
ATTTAGA5050.017.7578221
GTCCTAA7500.017.5083681
TAGCGGC1750.017.3710730
ATGTCCA5600.017.1443889
TTTAGAA5800.016.8300132
AAAGTGT8200.016.5813796
AAATGTC5650.016.4235367
GAAATGT5650.016.420836
GTAGTAC703.671246E-416.0137521
TGTCCAC6300.016.00142910
TAGAAAT5500.015.9961554
TTAGCCA805.6303605E-515.9961544
AAGTGTG9100.015.8229797
CTAAAGT8450.015.7121984
TTAGAAA5600.015.4265583
CGATAAC1255.1266397E-815.36137210
AATGTCC6150.015.34927758
Produced by FastQC (version 0.11.5)