##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062719_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910107
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183052102664853	32.0	32.0	32.0	32.0	32.0
2	30.892132463545494	32.0	32.0	32.0	32.0	32.0
3	30.951550751724795	32.0	32.0	32.0	32.0	32.0
4	30.98550390228841	32.0	32.0	32.0	32.0	32.0
5	30.966511629951203	32.0	32.0	32.0	32.0	32.0
6	34.60394547014801	36.0	36.0	36.0	32.0	36.0
7	34.51102562665709	36.0	36.0	36.0	32.0	36.0
8	34.47542541701141	36.0	36.0	36.0	32.0	36.0
9	34.568741917159194	36.0	36.0	36.0	32.0	36.0
10	34.359028114276676	36.0	36.0	36.0	32.0	36.0
11	34.59303686269856	36.0	36.0	36.0	32.0	36.0
12	34.44198099783871	36.0	36.0	36.0	32.0	36.0
13	34.50681403395425	36.0	36.0	36.0	32.0	36.0
14	34.43642121201133	36.0	36.0	36.0	32.0	36.0
15	34.39932667257806	36.0	36.0	36.0	32.0	36.0
16	34.41349753380646	36.0	36.0	36.0	32.0	36.0
17	34.34314866273966	36.0	36.0	36.0	32.0	36.0
18	34.3717969425573	36.0	36.0	36.0	32.0	36.0
19	34.34306735361886	36.0	36.0	36.0	32.0	36.0
20	34.28595648643511	36.0	36.0	36.0	32.0	36.0
21	34.25662147417831	36.0	36.0	36.0	32.0	36.0
22	34.238333514630696	36.0	36.0	36.0	32.0	36.0
23	34.20822167063873	36.0	36.0	36.0	32.0	36.0
24	34.1976954358114	36.0	36.0	36.0	32.0	36.0
25	34.164597129788035	36.0	36.0	36.0	32.0	36.0
26	34.13693774468277	36.0	36.0	36.0	32.0	36.0
27	34.12675872177667	36.0	36.0	36.0	32.0	36.0
28	34.091649663171474	36.0	36.0	36.0	32.0	36.0
29	34.047165882692916	36.0	36.0	36.0	32.0	36.0
30	34.00545540249663	36.0	36.0	36.0	32.0	36.0
31	34.02460919430353	36.0	36.0	36.0	32.0	36.0
32	33.965665575586165	36.0	36.0	36.0	32.0	36.0
33	33.94686668710382	36.0	36.0	36.0	32.0	36.0
34	33.931074038547116	36.0	36.0	36.0	32.0	36.0
35	33.88247975238077	36.0	36.0	36.0	32.0	36.0
36	33.83381844112835	36.0	36.0	36.0	32.0	36.0
37	33.807640200547844	36.0	36.0	36.0	32.0	36.0
38	33.19374205450568	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	7.0
10	3.0
11	13.0
12	3.0
13	7.0
14	163.0
15	338.0
16	422.0
17	497.0
18	615.0
19	855.0
20	1066.0
21	1388.0
22	1926.0
23	2850.0
24	4052.0
25	5811.0
26	8590.0
27	12152.0
28	17264.0
29	23006.0
30	31336.0
31	41599.0
32	56397.0
33	83679.0
34	190084.0
35	425981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42575391355146	18.173246919271207	12.263506414494856	25.137492752682473
2	15.214879458401082	20.238829467361036	40.48832292769483	24.05796814654305
3	18.388464036378462	25.633057102888056	29.116725586792807	26.861753273940675
4	11.782079977848028	16.542226646756262	37.107189831190105	34.5685035442056
5	13.449012420558528	38.002140414721836	34.02369399541151	14.525153169308123
6	32.04245663961851	36.78740365122706	18.05011509660973	13.120024612544706
7	28.923224327715673	31.315912651932855	21.768782976927962	17.992080043423513
8	27.085176073477395	34.756285826988815	19.883353862145274	18.275184237388515
9	27.85852574766345	14.431569208726055	19.41024192892665	38.299663114683845
10	16.32566055855339	27.08018121065684	32.12137993475449	24.472778296035276
11	36.17766115953113	21.05556361334898	24.175467974666297	18.5913072524536
12	25.54206832593654	24.024617643083733	29.902459017294102	20.530855013685624
13	30.0019121047218	20.593477744859296	25.488136159438763	23.91647399098014
14	22.885101426342278	21.619854508692114	25.86668424869783	29.628359816267775
15	23.899654466004904	30.22114615296693	22.98867123275627	22.89052814827189
16	23.69946769932704	26.151413387955323	26.923271341476816	23.225847571240816
17	22.415278638655536	27.16358728860872	27.07951433306518	23.341619739670566
18	23.43652134928257	24.812483654816088	29.754717851907692	21.99627714399365
19	25.126671787382598	25.125133374870472	26.535857648492524	23.21233718925441
20	25.62172271474597	24.743531138879977	26.824511126836708	22.810235019537345
21	26.087553719752936	24.48675727737139	25.19748979997736	24.228199202898313
22	25.27515153113476	26.38960314006795	25.559972924386077	22.775272404411215
23	22.57093250697787	26.21414913957935	27.099184633304763	24.115733720138017
24	24.312655015950778	25.861798025686085	27.06928780301257	22.756259155350573
25	23.88031161706575	25.75357459589016	27.13121302734287	23.23490075970122
26	22.34127664437968	27.105834619121094	27.906539714357304	22.646349022141923
27	23.720640948720146	26.480452206860022	26.611437475412792	23.187469369007037
28	22.54907993661859	26.573052351199827	26.689859633469077	24.188008078712507
29	23.25947089365677	25.951704623499356	26.679267053102933	24.109557429740942
30	22.51433393621206	26.296756981269738	27.59862341469632	23.590285667821878
31	24.252543533178837	25.65663758044861	26.084634631169834	24.00618425520272
32	22.830745157334434	27.03057901006114	26.17896434866313	23.959711483941295
33	22.0958764869097	26.722452679871434	27.605725117441825	23.57594571577704
34	23.276010800035603	26.603604187706114	27.567343628605368	22.553041383652914
35	23.79597017467705	26.584099388438016	27.28511536157984	22.334815075305094
36	22.388404140750346	27.001692344886703	27.09334271082881	23.51656080353414
37	23.51072445421709	27.11140267883339	25.792644801285242	23.585228065664275
38	22.861001349373442	27.68753873405036	26.212677956864006	23.238781959712192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	773.0
1	592.0
2	411.0
3	411.0
4	809.0
5	1207.0
6	1207.0
7	1360.0
8	1513.0
9	1444.5
10	1376.0
11	1376.0
12	1754.5
13	2133.0
14	2579.0
15	3025.0
16	3025.0
17	4521.5
18	6018.0
19	6018.0
20	6915.0
21	7812.0
22	8022.5
23	8233.0
24	8233.0
25	10276.5
26	12320.0
27	12320.0
28	16777.5
29	21235.0
30	28033.5
31	34832.0
32	34832.0
33	42953.5
34	51075.0
35	51075.0
36	54354.0
37	57633.0
38	62146.5
39	66660.0
40	66660.0
41	65414.0
42	64168.0
43	67499.0
44	70830.0
45	70830.0
46	71531.5
47	72233.0
48	72233.0
49	74066.5
50	75900.0
51	73780.0
52	71660.0
53	71660.0
54	66999.5
55	62339.0
56	62339.0
57	59992.5
58	57646.0
59	51262.5
60	44879.0
61	44879.0
62	43025.0
63	41171.0
64	33775.0
65	26379.0
66	26379.0
67	22008.5
68	17638.0
69	17638.0
70	14338.0
71	11038.0
72	8620.0
73	6202.0
74	6202.0
75	4728.0
76	3254.0
77	3254.0
78	3153.5
79	3053.0
80	2404.0
81	1755.0
82	1755.0
83	1785.5
84	1816.0
85	1816.0
86	1206.5
87	597.0
88	525.5
89	454.0
90	454.0
91	325.0
92	196.0
93	175.0
94	154.0
95	154.0
96	136.0
97	118.0
98	118.0
99	244.5
100	371.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12635876880410765
2	0.05537810389327848
3	0.01296550845120409
4	0.0032963157079332426
5	3.296315707933243E-4
6	2.1975438052888286E-4
7	1.0987719026444143E-4
8	4.395087610577657E-4
9	5.493859513222072E-4
10	6.592631415866486E-4
11	0.0016481578539666213
12	0.0030765613274043604
13	0.012635876880410764
14	0.009559315553006405
15	0.023184087145797144
16	0.010438333075121936
17	0.02010752581839278
18	0.005823491084015395
19	0.008570420840626432
20	0.006043245464544279
21	0.006592631415866485
22	0.00692226298665981
23	0.009559315553006405
24	0.01318526283173297
25	0.01582231539807957
26	0.017470473252046186
27	0.008900052411419756
28	0.006702508606130927
29	0.009009929601684197
30	0.0031864385176688017
31	0.005933368274279837
32	0.007801280508775342
33	0.009009929601684197
34	0.012086490929088558
35	0.015602561017550684
36	0.013954403163584062
37	0.00922968398221308
38	0.006043245464544279
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	910107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.02315340316781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14777055813774	43.549599209391424
2	13.176175905305094	14.499894962094514
3	3.86597059258873	6.3815367890443575
4	1.452534775645789	3.1969217513517676
5	0.6958802633419293	1.9144763240049896
6	0.3808977935505497	1.2574918635276024
7	0.20691824156121832	0.7969705903135653
8	0.13755012045365617	0.6054753102676588
9	0.10143708374159867	0.5023249397535566
>10	0.6297496381271691	7.003979263979613
>50	0.09443884957023622	3.7251065983420393
>100	0.09944815994277678	11.068676438537931
>500	0.008421013525154048	3.119674198098802
>1k	0.002807004508384683	2.3778717612922264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3882	0.4265432526065617	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2791	0.30666723802805607	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	1603	0.17613313599389963	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1476	0.16217873283031556	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1463	0.1607503293568778	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1345	0.14778482090567374	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	1328	0.14591690867117824	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1220	0.13405017212261855	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.13009459327309866	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1122	0.12328220747670328	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1068	0.11734883920242345	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1036	0.11383276911396131	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1019	0.11196485687946583	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1017	0.11174510249893693	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	962	0.10570185703439267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.296315707933243E-4	0.0
17	0.0	0.0	0.0	3.296315707933243E-4	0.0
18	0.0	0.0	0.0	6.592631415866486E-4	0.0
19	0.0	0.0	0.0	6.592631415866486E-4	0.0
20	0.0	0.0	0.0	8.790175221155314E-4	0.0
21	0.0	0.0	0.0	0.0012086490929088557	0.0
22	0.0	0.0	0.0	0.0017580350442310628	0.0
23	0.0	0.0	0.0	0.0031864385176688017	0.0
24	0.0	0.0	0.0	0.005164227942428747	0.0
25	0.0	0.0	0.0	0.006153122654808721	1.0987719026444143E-4
26	0.0	0.0	0.0	0.007581526128246459	1.0987719026444143E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	40	0.0044861613	19.997389	3
TCTAGCG	160	0.0	18.999609	28
CTAGCGG	165	0.0	18.423862	29
ATTTAGA	505	0.0	17.757822	1
GTCCTAA	750	0.0	17.508368	1
TAGCGGC	175	0.0	17.37107	30
ATGTCCA	560	0.0	17.144388	9
TTTAGAA	580	0.0	16.830013	2
AAAGTGT	820	0.0	16.581379	6
AAATGTC	565	0.0	16.423536	7
GAAATGT	565	0.0	16.42083	6
GTAGTAC	70	3.671246E-4	16.013752	1
TGTCCAC	630	0.0	16.001429	10
TAGAAAT	550	0.0	15.996155	4
TTAGCCA	80	5.6303605E-5	15.996154	4
AAGTGTG	910	0.0	15.822979	7
CTAAAGT	845	0.0	15.712198	4
TTAGAAA	560	0.0	15.426558	3
CGATAAC	125	5.1266397E-8	15.361372	10
AATGTCC	615	0.0	15.3492775	8
>>END_MODULE