FastQCFastQC Report
Thu 2 Feb 2017
SRR4062719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910107
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44820.49246956676522646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34420.37819728889020743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19970.21942474895808953No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA15550.17085903086120643No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA14600.1604206977860845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.15624536455603572No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13160.14459838238800493No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13120.14415887362694715No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG12950.14229096139245168No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC11400.12525999690146322No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11060.12152417243247224No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10130.11130559373787917No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9940.10921792712285479No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9850.10822903241047481No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9660.10614136579545043No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9540.10482283951227714No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9330.10251541851672387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2250.022.7564548
ATACCGT2600.020.9239046
TACCGTC2450.020.8987857
GTCGTAG2550.020.07922411
CCGTCGT2650.019.9253169
CGTCGTA2750.019.782610
TAGTGCA659.431533E-619.6920054
GTTTTCG2100.018.28543328
GGCAACG450.00884968317.77750420
CGGTCAA450.00884968317.77750430
CCCCTAT550.001363486817.4561941
CCAACGA1750.017.3711629
TAACCCG652.1000457E-417.23050328
TTTTCGG2250.017.06640229
CTAGCGG1700.016.94091429
CAGATAC3150.016.7616443
TAGGACG14250.016.6172874
AATCCCG1456.91216E-1116.55146819
TTAGGAC8350.016.4787883
TAGCGGC1750.016.45688830
Produced by FastQC (version 0.11.5)