##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910107
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14630807146852	32.0	32.0	32.0	32.0	32.0
2	31.316896804441676	32.0	32.0	32.0	32.0	32.0
3	31.397558748586704	32.0	32.0	32.0	32.0	32.0
4	31.492398146591555	32.0	32.0	32.0	32.0	32.0
5	31.40563472207114	32.0	32.0	32.0	32.0	32.0
6	34.92075217529367	36.0	36.0	36.0	36.0	36.0
7	34.9664698766189	36.0	36.0	36.0	36.0	36.0
8	34.8970791346512	36.0	36.0	36.0	36.0	36.0
9	34.999407761944475	36.0	36.0	36.0	36.0	36.0
10	34.86448406615926	36.0	36.0	36.0	32.0	36.0
11	35.02182820261793	36.0	36.0	36.0	36.0	36.0
12	34.92227177683503	36.0	36.0	36.0	32.0	36.0
13	34.98320307392427	36.0	36.0	36.0	36.0	36.0
14	34.91781845431361	36.0	36.0	36.0	32.0	36.0
15	34.897106603948764	36.0	36.0	36.0	32.0	36.0
16	34.9093776885575	36.0	36.0	36.0	32.0	36.0
17	34.879387808246726	36.0	36.0	36.0	32.0	36.0
18	34.875749774477065	36.0	36.0	36.0	32.0	36.0
19	34.85358424888502	36.0	36.0	36.0	32.0	36.0
20	34.83064299032971	36.0	36.0	36.0	32.0	36.0
21	34.81208912798166	36.0	36.0	36.0	32.0	36.0
22	34.80098823544924	36.0	36.0	36.0	32.0	36.0
23	34.74270497864537	36.0	36.0	36.0	32.0	36.0
24	34.725693792048624	36.0	36.0	36.0	32.0	36.0
25	34.701633983696425	36.0	36.0	36.0	32.0	36.0
26	34.63999727504568	36.0	36.0	36.0	32.0	36.0
27	34.619355746082604	36.0	36.0	36.0	32.0	36.0
28	34.56728714316009	36.0	36.0	36.0	32.0	36.0
29	34.527463254320644	36.0	36.0	36.0	32.0	36.0
30	34.49306619990836	36.0	36.0	36.0	32.0	36.0
31	34.473771765297926	36.0	36.0	36.0	32.0	36.0
32	34.438759398620164	36.0	36.0	36.0	32.0	36.0
33	34.423730396535795	36.0	36.0	36.0	32.0	36.0
34	34.37792808977406	36.0	36.0	36.0	32.0	36.0
35	34.34861505295531	36.0	36.0	36.0	32.0	36.0
36	34.305335526482054	36.0	36.0	36.0	32.0	36.0
37	34.26634450674481	36.0	36.0	36.0	32.0	36.0
38	33.86007139819824	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	5.0
21	13.0
22	80.0
23	202.0
24	586.0
25	1472.0
26	3224.0
27	6220.0
28	10921.0
29	17454.0
30	26418.0
31	37727.0
32	53474.0
33	84469.0
34	203559.0
35	464280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.741664523870746	19.355490942017042	12.951717863006113	25.9511266711061
2	14.42709483610169	21.228712667851145	39.536010600951315	24.808181895095853
3	17.504315426647636	26.22548777231688	29.659809231222262	26.61038756981322
4	11.455930909070931	17.39381719691684	37.138901555315044	34.011350338697184
5	12.948587363903366	38.55766409883673	33.746251814347104	14.7474967229128
6	32.18581132307665	37.139300257332664	17.62344166503682	13.051446754553867
7	28.493540826787356	31.598361061025095	21.829445303323478	18.07865280886407
8	26.84555720252151	35.13077263702201	19.649463625066065	18.374206535390417
9	27.50928224281402	14.293390295921968	19.362665110744107	38.834662350519906
10	16.160219932117275	27.428884110409967	32.00223272413221	24.40866323334055
11	36.185617306876644	20.94221737764074	24.20461812659741	18.6675471888852
12	25.41816603578708	24.072277374588648	29.87226748561979	20.637289104004484
13	29.8969899077579	20.48840518401723	25.603749017970458	24.01085589025442
14	22.763037752703802	21.572628273378843	25.893658657718266	29.770675316199085
15	23.988717810103648	30.185461709447353	22.90818552104313	22.917634959405873
16	23.600741451279905	26.137256388534535	26.86046805485509	23.40153410533047
17	22.438570409852908	27.267563044784843	26.942216684411836	23.351649860950417
18	23.408566245507394	24.66292424956626	29.769686421486703	22.15882308343964
19	25.144186342924513	24.997720048302014	26.702904164015877	23.155189444757596
20	25.56314806940283	24.743574107220358	26.88475091390353	22.80852690947328
21	25.961509935666765	24.45256316578856	25.415309222562232	24.17061767598244
22	25.1530041555599	26.24474511760169	25.87940305854483	22.722847668293582
23	22.414067796423936	25.936400884731135	27.351728972527408	24.29780234631752
24	24.23989706704816	25.67884875075129	27.154719170383263	22.926535011817293
25	23.888509812582477	25.571608613053193	27.234270256134717	23.305611318229616
26	22.25630860581075	26.977956413868277	27.92092349682345	22.844811483497526
27	23.75544853517224	26.176812177029735	26.755315583772017	23.31242370402601
28	22.5408660739891	26.351846541120988	26.95353403500907	24.153753349880837
29	23.281438336371437	25.516779895111235	26.976498367774337	24.22528340074299
30	22.527900565537898	25.80762481774121	27.84463804805369	23.819836568667203
31	24.153973104261368	25.260985796175618	26.50512522153988	24.079915878023133
32	22.91510778402979	26.574128097025955	26.406895013443478	24.103869105500785
33	22.182996065297818	26.320971050656684	27.837056521925447	23.658976362120058
34	23.39604024581725	26.20768766749404	27.775854926948153	22.62041715974056
35	23.883015953069254	26.27273496413059	27.317996675116223	22.52625240768393
36	22.462963146091614	26.600608499879684	27.348982042770796	23.587446311257906
37	23.686885168447226	26.70620047972381	25.927830463890512	23.67908388793845
38	23.18057107570868	27.106483083857174	26.419970399084942	23.292975441349203
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	489.0
1	343.5
2	198.0
3	198.0
4	641.5
5	1085.0
6	1085.0
7	1290.5
8	1496.0
9	1466.0
10	1436.0
11	1436.0
12	1799.5
13	2163.0
14	2702.0
15	3241.0
16	3241.0
17	4845.0
18	6449.0
19	6449.0
20	7233.5
21	8018.0
22	8299.5
23	8581.0
24	8581.0
25	10264.0
26	11947.0
27	11947.0
28	16492.0
29	21037.0
30	27617.5
31	34198.0
32	34198.0
33	42900.0
34	51602.0
35	51602.0
36	54398.5
37	57195.0
38	62003.0
39	66811.0
40	66811.0
41	65292.5
42	63774.0
43	67140.5
44	70507.0
45	70507.0
46	71621.0
47	72735.0
48	72735.0
49	74995.5
50	77256.0
51	74609.0
52	71962.0
53	71962.0
54	67173.5
55	62385.0
56	62385.0
57	60430.0
58	58475.0
59	51555.5
60	44636.0
61	44636.0
62	42810.5
63	40985.0
64	33653.5
65	26322.0
66	26322.0
67	22092.5
68	17863.0
69	17863.0
70	14431.5
71	11000.0
72	8593.0
73	6186.0
74	6186.0
75	4652.0
76	3118.0
77	3118.0
78	3070.0
79	3022.0
80	2344.0
81	1666.0
82	1666.0
83	1583.5
84	1501.0
85	1501.0
86	934.0
87	367.0
88	330.5
89	294.0
90	294.0
91	179.0
92	64.0
93	41.5
94	19.0
95	19.0
96	17.0
97	15.0
98	15.0
99	12.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008900052411419756
2	0.0
3	0.0
4	2.1975438052888286E-4
5	0.0
6	1.0987719026444143E-4
7	6.592631415866486E-4
8	0.0017580350442310628
9	0.003955578849519892
10	8.790175221155314E-4
11	0.0045049648008420986
12	2.1975438052888286E-4
13	2.1975438052888286E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.1975438052888286E-4
22	1.0987719026444143E-4
23	0.0
24	0.0
25	0.0
26	1.0987719026444143E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	910107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.13506097853664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69443179971441	41.28298371370581
2	14.124348075705262	15.009961925693515
3	4.105408739766977	6.544234311880068
4	1.513490148351877	3.2167756529236584
5	0.7625735616275161	2.025969634884897
6	0.3952655312327831	1.2601474862860567
7	0.25157540791065985	0.9357232248023201
8	0.1524040371957922	0.6478398247818868
9	0.11440127366887194	0.5470846787175991
>10	0.6645654958264425	7.042670025429901
>50	0.09964081955902368	3.7371291955106662
>100	0.10983040579687599	11.886666348447381
>500	0.009568558062086355	3.4820543610851455
>1k	0.002496145581413832	2.3807596158511313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4482	0.49246956676522646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3442	0.37819728889020743	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1997	0.21942474895808953	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1555	0.17085903086120643	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	1460	0.1604206977860845	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1422	0.15624536455603572	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1316	0.14459838238800493	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1312	0.14415887362694715	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	1295	0.14229096139245168	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1140	0.12525999690146322	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1106	0.12152417243247224	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1013	0.11130559373787917	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	994	0.10921792712285479	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	985	0.10822903241047481	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	966	0.10614136579545043	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	954	0.10482283951227714	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	933	0.10251541851672387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.296315707933243E-4	0.0
17	0.0	0.0	0.0	4.395087610577657E-4	0.0
18	0.0	0.0	0.0	6.592631415866486E-4	0.0
19	0.0	0.0	0.0	6.592631415866486E-4	0.0
20	0.0	0.0	0.0	8.790175221155314E-4	0.0
21	0.0	0.0	0.0	0.0012086490929088557	0.0
22	0.0	0.0	0.0	0.0017580350442310628	0.0
23	0.0	0.0	0.0	0.0034061928981976844	0.0
24	0.0	0.0	0.0	0.00538398232295763	0.0
25	0.0	0.0	0.0	0.006482754225602044	0.0
26	0.0	0.0	0.0	0.0076914033185109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	225	0.0	22.756454	8
ATACCGT	260	0.0	20.923904	6
TACCGTC	245	0.0	20.898785	7
GTCGTAG	255	0.0	20.079224	11
CCGTCGT	265	0.0	19.925316	9
CGTCGTA	275	0.0	19.7826	10
TAGTGCA	65	9.431533E-6	19.692005	4
GTTTTCG	210	0.0	18.285433	28
GGCAACG	45	0.008849683	17.777504	20
CGGTCAA	45	0.008849683	17.777504	30
CCCCTAT	55	0.0013634868	17.456194	1
CCAACGA	175	0.0	17.37116	29
TAACCCG	65	2.1000457E-4	17.230503	28
TTTTCGG	225	0.0	17.066402	29
CTAGCGG	170	0.0	16.940914	29
CAGATAC	315	0.0	16.761644	3
TAGGACG	1425	0.0	16.617287	4
AATCCCG	145	6.91216E-11	16.551468	19
TTAGGAC	835	0.0	16.478788	3
TAGCGGC	175	0.0	16.456888	30
>>END_MODULE