FastQCFastQC Report
Thu 2 Feb 2017
SRR4062718_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062718_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504610
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49890.11075320615991173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44300.021.6233141
GGTATCA14450.020.064831
ACCGTCG8000.015.5987228
TACCGTC8650.014.6113597
ACGATTA1654.8567017E-1014.54458732
TATCAAC66000.014.430432
ATACCGT9450.014.2188486
CGTCGTA8950.014.12300310
TCAACGC66200.014.1119244
AACCGCG3200.013.9986987
ATCAACG67000.013.9449733
CAACGCA67200.013.8778135
CCGTCGT9400.013.6171169
TAGGACG9850.013.4794834
AACGCAG68950.013.4790346
TCTAGCG6300.013.45906528
GTATTAG8300.013.3166741
GTAAACG5450.013.20978227
CTAGCGG6300.013.20570829
GTCCTAT7150.012.7700791
Produced by FastQC (version 0.11.5)